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. 1987 Apr;79(4):1104–1109. doi: 10.1172/JCI112925

Insulin stimulates volume absorption in the rabbit proximal convoluted tubule.

M Baum
PMCID: PMC424290  PMID: 3549779

Abstract

The present in vitro microperfusion study examined whether insulin affects volume absorption (Jv) in the proximal convoluted tubule (PCT). PCT were perfused with an ultrafiltrate-like solution and were bathed in a serum-like albumin solution. Addition of a physiologic concentration of 10(-10) M insulin to the bathing solution resulted in a stimulation of Jv and a more negative transepithelial potential difference (PD). There was a progressive stimulation of the lumen negative PD and Jv with higher insulin concentrations. Maximal stimulation occurred at 10(-8) M bath insulin. The insulin-induced stimulation of volume reabsorption was also observed when glucose and amino acids were removed from the luminal perfusate. Direct examination of the effect of insulin on glucose, chloride, and bicarbonate absorption demonstrated that the transport of all these solutes was stimulated by insulin. Addition of insulin to the luminal perfusate had no affect on Jv. These data show that insulin has a direct effect to stimulate Jv in the proximal tubule.

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Selected References

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  1. Atchley D. W., Loeb R. F., Richards D. W., Benedict E. M., Driscoll M. E. ON DIABETIC ACIDOSIS: A Detailed Study of Electrolyte Balances Following the Withdrawal and Reestablishment of Insulin Therapy. J Clin Invest. 1933 Mar;12(2):297–326. doi: 10.1172/JCI100504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baum M., Berry C. A. Evidence for neutral transcellular NaCl transport and neutral basolateral chloride exit in the rabbit proximal convoluted tubule. J Clin Invest. 1984 Jul;74(1):205–211. doi: 10.1172/JCI111403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baum M., Berry C. A. Peritubular protein modulates neutral active NaCl absorption in rabbit proximal convoluted tubule. Am J Physiol. 1985 Jun;248(6 Pt 2):F790–F795. doi: 10.1152/ajprenal.1985.248.6.F790. [DOI] [PubMed] [Google Scholar]
  4. Baum M., Toto R. D. Lack of a direct effect of atrial natriuretic factor in the rabbit proximal tubule. Am J Physiol. 1986 Jan;250(1 Pt 2):F66–F69. doi: 10.1152/ajprenal.1986.250.1.F66. [DOI] [PubMed] [Google Scholar]
  5. Blanchard R. F., Davis P. J., Blas S. D. Physical characteristics of insulin receptors on renal cell membranes. Diabetes. 1978 Feb;27(2):88–95. doi: 10.2337/diab.27.2.88. [DOI] [PubMed] [Google Scholar]
  6. Burg M., Grantham J., Abramow M., Orloff J. Preparation and study of fragments of single rabbit nephrons. Am J Physiol. 1966 Jun;210(6):1293–1298. doi: 10.1152/ajplegacy.1966.210.6.1293. [DOI] [PubMed] [Google Scholar]
  7. DeFronzo R. A., Cooke C. R., Andres R., Faloona G. R., Davis P. J. The effect of insulin on renal handling of sodium, potassium, calcium, and phosphate in man. J Clin Invest. 1975 Apr;55(4):845–855. doi: 10.1172/JCI107996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DeFronzo R. A., Goldberg M., Agus Z. S. The effects of glucose and insulin on renal electrolyte transport. J Clin Invest. 1976 Jul;58(1):83–90. doi: 10.1172/JCI108463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fine L. G., Badie-Dezfooly B., Lowe A. G., Hamzeh A., Wells J., Salehmoghaddam S. Stimulation of Na+/H+ antiport is an early event in hypertrophy of renal proximal tubular cells. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1736–1740. doi: 10.1073/pnas.82.6.1736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. François B., de Gasparo M., Crabbé J. Interaction between isolated amphibian skin and insulin. Arch Int Physiol Biochim. 1969 Aug;77(3):527–530. [PubMed] [Google Scholar]
  11. Guntupalli J., Rogers A., Bourke E. Effect of insulin on renal phosphorus handling in the rat: interaction with PTH and nicotinamide. Am J Physiol. 1985 Oct;249(4 Pt 2):F610–F618. doi: 10.1152/ajprenal.1985.249.4.F610. [DOI] [PubMed] [Google Scholar]
  12. HERRERA F. C., WHITTEMBURY G., PLANCHART A. Effect of insulin on short-circuit current across isolated frog skin in the presence of calcium and magnesium. Biochim Biophys Acta. 1963 Jan 15;66:170–172. doi: 10.1016/0006-3002(63)91182-x. [DOI] [PubMed] [Google Scholar]
  13. Hammerman M. R., Gavin J. R., 3rd Insulin-stimulated phosphorylation and insulin binding in canine renal basolateral membranes. Am J Physiol. 1984 Sep;247(3 Pt 2):F408–F417. doi: 10.1152/ajprenal.1984.247.3.F408. [DOI] [PubMed] [Google Scholar]
  14. Hammerman M. R., Rogers S., Hansen V. A., Gavin J. R., 3rd Insulin stimulates Pi transport in brush border vesicles from proximal tubular segments. Am J Physiol. 1984 Nov;247(5 Pt 1):E616–E624. doi: 10.1152/ajpendo.1984.247.5.E616. [DOI] [PubMed] [Google Scholar]
  15. Herrera F. C. Effect of insulin on short-circuit current and sodium transport across toad urinary bladder. Am J Physiol. 1965 Oct;209(4):819–824. doi: 10.1152/ajplegacy.1965.209.4.819. [DOI] [PubMed] [Google Scholar]
  16. King G. L., Rechler M. M., Kahn C. R. Interactions between the receptors for insulin and the insulin-like growth factors on adipocytes. J Biol Chem. 1982 Sep 10;257(17):10001–10006. [PubMed] [Google Scholar]
  17. Kurokawa K., Lerner R. L. Binding and degradation of insulin by isolated renal cortical tubules. Endocrinology. 1980 Mar;106(3):655–662. doi: 10.1210/endo-106-3-655. [DOI] [PubMed] [Google Scholar]
  18. Lau K., Guntupalli J., Eby B. Effects of somatostatin on phosphate transport: evidence for the role of basal insulin. Kidney Int. 1983 Jul;24(1):10–15. doi: 10.1038/ki.1983.120. [DOI] [PubMed] [Google Scholar]
  19. Moore R. D. Effects of insulin upon ion transport. Biochim Biophys Acta. 1983 Mar 21;737(1):1–49. doi: 10.1016/0304-4157(83)90013-8. [DOI] [PubMed] [Google Scholar]
  20. Nakamura R., Emmanouel D. S., Katz A. I. Insulin binding sites in various segments of the rabbit nephron. J Clin Invest. 1983 Jul;72(1):388–392. doi: 10.1172/JCI110979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nizet A., Lefebvre P., Crabbé J. Control by insulin of sodium potassium and water excretion by the isolated dog kidney. Pflugers Arch. 1971;323(1):11–20. doi: 10.1007/BF00586561. [DOI] [PubMed] [Google Scholar]
  22. Oppenheimer C. L., Pessin J. E., Massague J., Gitomer W., Czech M. P. Insulin action rapidly modulates the apparent affinity of the insulin-like growth factor II receptor. J Biol Chem. 1983 Apr 25;258(8):4824–4830. [PubMed] [Google Scholar]
  23. Saudek C. D., Boulter P. R., Knopp R. H., Arky R. A. Sodium retention accompanying insulin treatment of diabetes mellitus. Diabetes. 1974 Mar;23(3):240–246. doi: 10.2337/diab.23.3.240. [DOI] [PubMed] [Google Scholar]
  24. Talor Z., Emmanouel D. S., Katz A. I. Insulin binding and degradation by luminal and basolateral tubular membranes from rabbit kidney. J Clin Invest. 1982 May;69(5):1136–1146. doi: 10.1172/JCI110549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vurek G. G., Warnock D. G., Corsey R. Measurement of picomole amounts of carbon dioxide by calorimetry. Anal Chem. 1975 Apr;47(4):765–767. doi: 10.1021/ac60354a024. [DOI] [PubMed] [Google Scholar]

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