Figure 2.

Genetic transformation and regeneration of peanut from deembryonated cotyledons. (a)-(b) Deembryonated cotyledons cocultured with Agrobacterium strain LBA4404; (c) shoot buds initiating from cocultured deembryonated cotyledons; (d) sequential regeneration process of shoots from deembryonated cotyledon explant in various concentration media; (e) transformed (green) and nontransformed shoots (yellow) in Kanamycin selection media; (f) transformed healthy shoots in Kanamycin selection media containing NAA; and (g) transformed groundnut plant with well-developed shoots transferred in pots for hardening.