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. Author manuscript; available in PMC: 2014 Nov 25.
Published in final edited form as: Euphytica. 2012 Nov 1;188(1):35–49. doi: 10.1007/s10681-012-0669-1

Table 2.

Germination percentage and Chi-square analysis of F2 segregation data for two ScABI mutants with accompanying parental germination

Genotype Generation n Germination (%)a χ2b P value
ScABI3/Scarlet F2 450 52.0 6.522 0.843
    ScABI3 parent M6 150 90.7
    Scarlet parent 150 1.3
ScABI4/Scarlet F2 254 42.9 0.039 0.011
    ScABI4 parent M7 71 81.7
    Scarlet parent 105 2.9
a

ScABI3 segregation analysis and parental germination testing was performed on intact seeds after-ripened for 6 weeks and treated with 2 µM ABA. ScABI4 segregation analysis and parental germination testing was performed on half seeds after-ripened for 3 weeks and treated with 5 µM ABA. Germination percentages after 112 h of imbibition are shown for ScABI3, and germination percentages after 48 h of imbibition are shown for ScABI4

b

Chi-square values calculated based on a hypothesis of incomplete dominance. Calculations were based on parental percentages using the midparent value to estimate germination of the heterozygous state