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. 1987 May;79(5):1529–1532. doi: 10.1172/JCI112984

Immunological regulation of synaptic transmission in isolated guinea pig autonomic ganglia.

D Weinreich, B J Undem
PMCID: PMC424430  PMID: 2437156

Abstract

We have attempted to identify a role for mast cells in autonomic ganglia by examining the effects of antigen challenge on mast cell-associated mediator release and synaptic transmission through the superior cervical ganglion isolated from ovalbumin-sensitized guinea pigs. Ovalbumin induced the release of 7.9 ng of histamine, 40 pg of immunoreactive sulfidopeptide-leukotriene, and 140 pg of immunoreactive-PgD2 per ganglion. Ovalbumin produced long-lasting potentiation (51 +/- 4%, mean +/- SEM, n = 66) of synaptic transmission, the protracted nature of which could not be mimicked by exogenous histamine (10(-5) M). Selective histamine H1 antagonists inhibited the antigen-induced potentiation, but did not reverse it when added any time after antigen exposure. These results indicate that immunologic activation of mast cells can directly potentiate neurotransmission in sympathetic ganglia. Histamine appears to be a mediator involved in the induction of antigen-induced potentiation of synaptic transmission, but alone cannot account for the long term nature of this phenomenon.

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Selected References

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  1. Adkinson N. F., Jr Prostaglandin production by human peripheral blood cells in vitro. J Lab Clin Med. 1977 Dec;90(6):1043–1053. [PubMed] [Google Scholar]
  2. Aharony D., Dobson P., Bernstein P. R., Kusner E. J., Krell R. D., Smith J. B. Determination of SRS-A release from guinea-pig lungs by a radioimmunoassay. Biochem Biophys Res Commun. 1983 Dec 16;117(2):574–579. doi: 10.1016/0006-291x(83)91239-1. [DOI] [PubMed] [Google Scholar]
  3. Briggs C. A., Brown T. H., McAfee D. A. Neurophysiology and pharmacology of long-term potentiation in the rat sympathetic ganglion. J Physiol. 1985 Feb;359:503–521. doi: 10.1113/jphysiol.1985.sp015599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown M. J., Ind P. W., Barnes P. J., Jenner D. A., Dollery C. T. A sensitive and specific radiometric method for the measurement of plasma histamine in normal individuals. Anal Biochem. 1980 Nov 15;109(1):142–146. doi: 10.1016/0003-2697(80)90022-6. [DOI] [PubMed] [Google Scholar]
  5. Casale T. B. The role of the autonomic nervous system in allergic diseases. Ann Allergy. 1983 Oct;51(4):423–429. [PubMed] [Google Scholar]
  6. Goetzl E. J., Chernov T., Renold F., Payan D. G. Neuropeptide regulation of the expression of immediate hypersensitivity. J Immunol. 1985 Aug;135(2 Suppl):802s–805s. [PubMed] [Google Scholar]
  7. Kaliner M., Orange R. P., Austen K. F. Immunological release of histamine and slow reacting substance of anaphylaxis from human lung. J Exp Med. 1972 Sep 1;136(3):556–567. doi: 10.1084/jem.136.3.556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaliner M., Shelhamer J. H., Davis P. B., Smith L. J., Venter J. C. Autonomic nervous system abnormalities and allergy. Ann Intern Med. 1982 Mar;96(3):349–357. doi: 10.7326/0003-4819-96-3-349. [DOI] [PubMed] [Google Scholar]
  9. Metcalfe D. D., Kaliner M., Donlon M. A. The mast cell. Crit Rev Immunol. 1981 Sep;3(1):23–74. [PubMed] [Google Scholar]
  10. Olsson Y. Mast cells in the nervous system. Int Rev Cytol. 1968;24:27–70. doi: 10.1016/s0074-7696(08)61396-0. [DOI] [PubMed] [Google Scholar]
  11. Schwartz L. B., Austen K. F. Structure and function of the chemical mediators of mast cells. Prog Allergy. 1984;34:271–321. [PubMed] [Google Scholar]
  12. Siraganian R. P. An automated continuous-flow system for the extraction and fluorometric analysis of histamine. Anal Biochem. 1974 Feb;57(2):383–394. doi: 10.1016/0003-2697(74)90093-1. [DOI] [PubMed] [Google Scholar]
  13. Undem B. J., Buckner C. K., Harley P., Graziano F. M. Smooth muscle contraction and release of histamine and slow-reacting substance of anaphylaxis in pulmonary tissues isolated from guinea pigs passively sensitized with IgG1 or IgE antibodies. Am Rev Respir Dis. 1985 Feb;131(2):260–266. doi: 10.1164/arrd.1985.131.2.260. [DOI] [PubMed] [Google Scholar]
  14. Weinreich D. Multiple sites of histamine storage in superior cervical ganglia. Exp Neurol. 1985 Oct;90(1):36–43. doi: 10.1016/0014-4886(85)90038-x. [DOI] [PubMed] [Google Scholar]
  15. Widdicombe J. G. Mediators and reflex bronchoconstriction. Eur J Respir Dis Suppl. 1983;129:65–94. [PubMed] [Google Scholar]
  16. Wong S. K., Buckner C. K. Studies on the beta adrenergic receptors mediating inhibition of antigen-induced histamine release from the lung and heart isolated from the actively sensitized guinea pig. J Pharmacol Exp Ther. 1980 Jul;214(1):152–160. [PubMed] [Google Scholar]

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