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. 1986 Apr;77(4):1402–1404. doi: 10.1172/JCI112449

Glutathione peroxidase protein. Absence in selenium deficiency states and correlation with enzymatic activity.

K Takahashi, P E Newburger, H J Cohen
PMCID: PMC424509  PMID: 3457020

Abstract

Glutathione peroxidase (GSHPx) activity is an indicator of selenium status in selenium-deficient individuals. Utilizing polyclonal monospecific antibodies to purified erythrocyte GSHPx, we were able to determine the relationship between enzymatic activity and protein content. In erythrocytes from a selenium-deficient individual who was treated with selenium, and in HL-60 cells grown in the absence of selenium and then returned to selenium-containing medium, there was a direct relationship between enzymatic activity and protein content. Thus, selenium deficiency results not only in a decrease of GSHPx activity, but also in a decrease of GSHPx protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Awasthi Y. C., Beutler E., Srivastava S. K. Purification and properties of human erythrocyte glutathione peroxidase. J Biol Chem. 1975 Jul 10;250(13):5144–5149. [PubMed] [Google Scholar]
  2. Baker S. S., Cohen H. J. Altered oxidative metabolism in selenium-deficient rat granulocytes. J Immunol. 1983 Jun;130(6):2856–2860. [PubMed] [Google Scholar]
  3. Baker S. S., Lerman R. H., Krey S. H., Crocker K. S., Hirsh E. F., Cohen H. Selenium deficiency with total parenteral nutrition: reversal of biochemical and functional abnormalities by selenium supplementation: a case report. Am J Clin Nutr. 1983 Nov;38(5):769–774. doi: 10.1093/ajcn/38.5.769. [DOI] [PubMed] [Google Scholar]
  4. Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 1973 Jul;133(3):529–539. doi: 10.1042/bj1330529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chow C. K., Tappel A. L. Response of glutathione peroxidase to dietary selenium in rats. J Nutr. 1974 Apr;104(4):444–451. doi: 10.1093/jn/104.4.444. [DOI] [PubMed] [Google Scholar]
  6. Cohen H. J., Chovaniec M. E., Mistretta D., Baker S. S. Selenium repletion and glutathione peroxidase--differential effects on plasma and red blood cell enzyme activity. Am J Clin Nutr. 1985 Apr;41(4):735–747. doi: 10.1093/ajcn/41.4.735. [DOI] [PubMed] [Google Scholar]
  7. Forstrom J. W., Zakowski J. J., Tappel A. L. Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteine. Biochemistry. 1978 Jun 27;17(13):2639–2644. doi: 10.1021/bi00606a028. [DOI] [PubMed] [Google Scholar]
  8. Hawkes W. C., Lyons D. E., Tappel A. L. Identification of a selenocysteine-specific aminoacyl transfer RNA from rat liver. Biochim Biophys Acta. 1982 Dec 31;699(3):183–191. doi: 10.1016/0167-4781(82)90106-3. [DOI] [PubMed] [Google Scholar]
  9. Johnson R. A., Baker S. S., Fallon J. T., Maynard E. P., 3rd, Ruskin J. N., Wen Z., Ge K., Cohen H. J. An occidental case of cardiomyopathy and selenium deficiency. N Engl J Med. 1981 May 14;304(20):1210–1212. doi: 10.1056/NEJM198105143042005. [DOI] [PubMed] [Google Scholar]
  10. Kien C. L., Ganther H. E. Manifestations of chronic selenium deficiency in a child receiving total parenteral nutrition. Am J Clin Nutr. 1983 Feb;37(2):319–328. doi: 10.1093/ajcn/37.2.319. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lane H. W., Dudrick S., Warren D. C. Blood selenium levels and glutathione-peroxidase activities in university and chronic intravenous hyperalimentation subjects. Proc Soc Exp Biol Med. 1981 Jul;167(3):383–390. doi: 10.3181/00379727-167-41184. [DOI] [PubMed] [Google Scholar]
  14. Omaye S. T., Tappel A. L. Effect of dietary selenium on glutathione peroxidase in the chick. J Nutr. 1974 Jun;104(6):747–753. doi: 10.1093/jn/104.6.747. [DOI] [PubMed] [Google Scholar]
  15. Roberts R., Parker C. W. Radioimmunoassay of creatine kinase isoenzymes. Methods Enzymol. 1981;74(Pt 100):198–209. doi: 10.1016/0076-6879(81)74014-x. [DOI] [PubMed] [Google Scholar]
  16. Speier C., Baker S. S., Newburger P. E. Relationships between in vitro selenium supply, glutathione peroxidase activity, and phagocytic function in the HL-60 human myeloid cell line. J Biol Chem. 1985 Jul 25;260(15):8951–8955. [PubMed] [Google Scholar]
  17. Zakowski J. J., Forstrom J. W., Condell R. A., Tappel A. L. Attachment of selenocysteine in the catalytic site of glutathione peroxidase. Biochem Biophys Res Commun. 1978 Sep 14;84(1):248–253. doi: 10.1016/0006-291x(78)90289-9. [DOI] [PubMed] [Google Scholar]
  18. van Rij A. M., Thomson C. D., McKenzie J. M., Robinson M. F. Selenium deficiency in total parenteral nutrition. Am J Clin Nutr. 1979 Oct;32(10):2076–2085. doi: 10.1093/ajcn/32.10.2076. [DOI] [PubMed] [Google Scholar]

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