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. 2014 Nov 12;11:93. doi: 10.1186/s12977-014-0093-9

Figure 6.

Figure 6

ADAR1 proviral effect is mediated through inhibition of PKR activation. 293-T cells were transfected with 4 μg of (A) pACH or (B) pH6neo molecular clones and increasing amount (0, 5, 50, 500 ng) of ADAR1 plasmid. Western blot analyses were performed on 60 μg of proteins obtained from whole cell extracts using anti-ADAR1, anti-p24gag, anti-Tax1, anti-Tax2, anti-PKR, anti-phospho-PKR and anti-actin antibodies. (C, D): 293-T cells were transfected with siRNA directed against PKR (20nM) or with control siRNA. Twelve hours later cells were transfected with 20nM of the same siRNA together with 1.2 μg of (C) pACH, (D) pH6neo and 125 ng of an ADAR1 expression plasmid. Western blot analyses were performed on 60 μg of proteins obtained from whole cell extracts using anti-ADAR1, anti-p24gag, anti-PKR, anti-phospho-PKR and anti-actin antibodies.