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. 1995 Mar 14;92(6):2278–2282. doi: 10.1073/pnas.92.6.2278

Encoded reaction cassette for the highly sensitive detection of the making and breaking of chemical bonds.

H Fenniri 1, K D Janda 1, R A Lerner 1
PMCID: PMC42467  PMID: 7892261

Abstract

A reaction cassette has been designed for the highly sensitive detection of the making and breaking of chemical bonds. The system is envisioned as a companion device to be used in the search for antibody and other novel catalysts. The cassette also may have important clinical applications in the design of diagnostic reagents. In its fully encoded format, this methodology is capable of both detecting and decoding chemical events.

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Selected References

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  1. Baumann W. K., Bizzozero S. A., Dutler H. Kinetic investigation of the alpha-chymotrypsin-catalyzed hydrolysis of peptide substrates. The relationship between peptide-structure N-terminal to the cleaved bond and reactivity. Eur J Biochem. 1973 Nov 15;39(2):381–391. doi: 10.1111/j.1432-1033.1973.tb03136.x. [DOI] [PubMed] [Google Scholar]
  2. Birk Y. The Bowman-Birk inhibitor. Trypsin- and chymotrypsin-inhibitor from soybeans. Int J Pept Protein Res. 1985 Feb;25(2):113–131. doi: 10.1111/j.1399-3011.1985.tb02155.x. [DOI] [PubMed] [Google Scholar]
  3. Brenner S., Lerner R. A. Encoded combinatorial chemistry. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5381–5383. doi: 10.1073/pnas.89.12.5381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eigen M., Rigler R. Sorting single molecules: application to diagnostics and evolutionary biotechnology. Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5740–5747. doi: 10.1073/pnas.91.13.5740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Janda K. D. Tagged versus untagged libraries: methods for the generation and screening of combinatorial chemical libraries. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10779–10785. doi: 10.1073/pnas.91.23.10779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lewis C., Krämer T., Robinson S., Hilvert D. Medium effects in antibody-catalyzed reactions. Science. 1991 Aug 30;253(5023):1019–1022. doi: 10.1126/science.1887215. [DOI] [PubMed] [Google Scholar]
  7. Mirzabekov A. D. DNA sequencing by hybridization--a megasequencing method and a diagnostic tool? Trends Biotechnol. 1994 Jan;12(1):27–32. doi: 10.1016/0167-7799(94)90008-6. [DOI] [PubMed] [Google Scholar]
  8. Ogura M., Keller C., Koo K., Mitsuhashi M. Use of the fluorescent dye YOYO-1 to quantify oligonucleotides immobilized on plastic plates. Biotechniques. 1994 Jun;16(6):1032–1034. [PubMed] [Google Scholar]
  9. Ruzicka V., März W., Russ A., Gross W. Immuno-PCR with a commercially available avidin system. Science. 1993 Apr 30;260(5108):698–699. doi: 10.1126/science.8480182. [DOI] [PubMed] [Google Scholar]
  10. Sano T., Smith C. L., Cantor C. R. Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Science. 1992 Oct 2;258(5079):120–122. doi: 10.1126/science.1439758. [DOI] [PubMed] [Google Scholar]
  11. Serpersu E. H., Shortle D., Mildvan A. S. Kinetic and magnetic resonance studies of active-site mutants of staphylococcal nuclease: factors contributing to catalysis. Biochemistry. 1987 Mar 10;26(5):1289–1300. doi: 10.1021/bi00379a014. [DOI] [PubMed] [Google Scholar]
  12. Tawfik D. S., Green B. S., Chap R., Sela M., Eshhar Z. catELISA: a facile general route to catalytic antibodies. Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):373–377. doi: 10.1073/pnas.90.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]

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