FIG. 2.

Hypoxic conditioning of the microfluidic chip with NaHAsc. (a) Time evolution of partial O₂ pressure (pO₂) measured in the media reservoir in contact with jacket solutions containing 0.8 M NaHCO₃, 65 mM Na₂CO₃, and different concentration of NaHAsc. Observed typical pO₂ values at 10-min intervals are plotted as lines; the average values at 24, 48, and 72 h are plotted as representative values (N = 3, ±SD). (b) The relationship among the NaHAsc concentration, the media reservoir pO₂, and the Na₂CO₃ concentration when the media reservoir pCO₂ is 5% on Day 1. Interpolated Na₂CO₃ concentrations values at pCO₂ = 5% atm shown in (a) with corresponding NaHAsc concentrations were plotted.