Skip to main content
NCBI home page
The Scientific World Journal logoLink to The Scientific World Journal
. 2014 Nov 13;2014:673456. doi: 10.1155/2014/673456

Meiotic Studies in Some Species of Tribe Cichorieae (Asteraceae) from Western Himalayas

Raghbir Chand Gupta 1, Henna Goyal 1, Vijay Singh 1,*, Rajesh Kumar Goel 2
PMCID: PMC4247967  PMID: 25489603

Abstract

The present paper deals with meiotic studies in 15 species belonging to 6 genera of the tribe Cichorieae from various localities of Western Himalayas. The chromosome number has been reported for the first time in Hieracium crocatum (2n = 10) and Lactuca lessertiana (2n = 2x = 16). Further, intraspecific variability has been reported for the first time in H. umbellatum (2n = 2x = 10 and 2n = 6x = 54), Tragopogon dubius (2n = 2x = 14 and 2n = 4x = 28), and T. gracilis (2n = 2x = 14). The chromosome report of 2n = 2x = 10 in Youngia tenuifolia is made for the first time in India. Maximum numbers of the populations show laggards, chromosome stickiness, and cytomixis from early prophase to telophase-II, leading to the formation of aneuploid cells or meiocytes with double chromosome number. Such meiotic abnormalities produce unreduced pollen grains and the reduced pollen viability.

1. Introduction

The tribe Cichorieae (also known as Lactuceae) encompasses 95 genera and ca. 2500 species, primarily in temperate to subtropical zones of the Northern Hemisphere [1]. Members of the tribe are characterized by very important uniform characteristics, such as homogamous ligulate capitula and the presence of milky latex.

The presently investigated species are also known to have medicinal uses, such as Hieracium crocatum to cure gastric troubles, Lactuca dolichophylla to cure constipation, L. macrorhiza used as an ingredient to cure stomach ache, L. serriola to treat ailments of the urinary tract, and Taraxacum officinale used as diuretic and laxative [2–4].

Chromosome studies are valuable determinants in studying evolution. Many workers have studied the cytology of Indian Asteraceae, including members of tribe Cichorieae. Cytological studies on the family from Lahaul-Spiti area, an ecologically very fragile cold desert area of Western Himalayas, are almost lacking. As an attempt to fill this lacuna, the present investigations have been undertaken.

2. Materials and Methods

2.1. Plant Material

Exploratory surveys were made during the years from 2009 to 2013 in selected localities (Table 1) of Himachal Pradesh (Kullu and Lahaul-Spiti Districts). The cytologically worked-out plants were identified using regional floras and compared with the specimens deposited at the Herbarium of Botanical Survey of India, Northern Circle, Dehra Dun. The voucher specimens (Table 1) were deposited in the Herbarium, Department of Botany, Punjabi University, Patiala (PUN).

Table 1.

The table showing details on taxon, geographical coordination, accession number, average plant height, flowering-fruiting period, chromosome number (2n), ploidal level (x), and pollen fertility of tribe Cichorieae (Asteraceae) from Western Himalayas.

Taxon Locality with altitude (m) and geographical coordinates Accession number (PUP*) Average plant height (cm) Flowering-fruiting period Chromosome number (2n) Ploidal level (x) Pollen fertility (%)
Hieracium crocatum 
Bunge ex Ledeb.		 Trilokinath, Lahaul (H.P.), 2760
32°42′0′′ N, 76°41′0′′ E		 52760 42 ± 6.4 June–September 10‡ 2x 88.4
H. umbellatum L.              
 (P1) Jispa, Spiti (H.P.), 3142
32°38′0′′ N, 77°10′0′′ E		 52763 42 ± 2.3 June–September 10‡ 2x 91.0
 (P2) Keylong, Lahaul (H.P.), 3080
32°34′48′′ N, 77°1′48′′ E		 52774 46 ± 7.2 June–September 54‡ 6x 82.1
L. dissecta  D. Don. Hadimba, Temple, Manali, 2438
32°14′32′′ N, 77°11′14′′ E		 58562 16 ± 4.3 June–September 16 2x 88.1
L. dolichophylla  Kitamura Sissu, Lahaul (H.P.), 3130
32°29′0′′ N, 77°7′0′′ E		 57504 20 ± 5.2 June–September 16 2x 79.2
L. lessertiana  C. B. Clarke Batal, Spiti (H.P.), 3890
32°21′28′′ N 77°37′10′′ E		 52776 32 ± 7.2 June–September 16‡ 2x 70.2
L. macrorhiza 
(Royle) Hook. f.		 Chotadhara, Spiti (H.P.) 3800 57510 25 ± 4.6 June–September 16 2x 71.3
L. sativa L. Hadimba, Temple, Manali, 2438
32°14′32′′ N, 77°11′14′′ E		 58534 55 ± 2.4 March–November 18 2x 87.0
L. serriola L. Jispa, Spiti (H.P.), 3200
32°38′0′′ N, 77°10′0′′ E		 58535 55 ± 2.5 April–October 18 2x 76.0
Prenanthes brunoniana 
C. B. Clarke		 Tandi, Keylong (H.P.), 2573
32°34′40′′ N, 77°1′36′′ E		 52764 80 ± 13.1 June–August 16 2x 90.1
Taraxacum officinale L.              
 (P1) Marhi, Manali (H.P.), 3320
32°20′56′′ N, 77°13′4′′ E		 52687 27.1 ± 3.2 March–November 16 2x 86.2
 (P2) Keylong, Lahaul (H.P), 3080
32°34′48′′ N, 77°1′48′′ E		 52693 8.3 ± 7.9   32 4x 73.6
 (P3) Kibber, Spiti (H.P.), 4205
32°19′54′′ N, 78°0′32′′ E		 57491 17.2 ± 1.6   24 3x 56.3
Tragopogon dubius  Scop.              
 (P1) Keylong, Lahaul (H.P), 3080
32°34′48′′ N, 77°1′48′′ E		 57503 50 ± 2.5 June–September 14‡ 2x 83.0
 (P2) Lossar, Spiti (H.P.), 4079
32°24′49′′ N, 77°49′11′′ E		 57505 35 ± 7.3 June–September 28‡ 4x 90.3
T. gracilis  D. Don Koksar, Lahaul (H.P.), 3160
32°41′37′′ N, 77°23′54′′ E		 52768 25.2 ± 4.9 June–September 14‡ 2x 79.2
Youngia glauca  Edgew. Zingzingbar, Lahaul (H.P.), 4270
32°47′30′′ N, 77°19′28′′ E		 52773 73 ± 5.3 July-August 14 2x 83.0
Y. japonica  (L.) DC. Hadimba, Temple, Manali, 2438
32°14′32′′ N, 77°11′14′′ E		 57500 42 ± 4.7 June–September 16 2x 78.2
Y. tenuifolia  (Wild.) Babc. Jispa, Spiti (H.P.), 3200
32°38′0′′ N, 77°10′0′′ E		 52771 15 ± 4.2 June–September 10† 2x 87.2
Open in a new tab

*Herbarium, Punjabi University, Patiala. ‡First ever chromosomal report. †First cytotype report from India.

2.2. Meiotic Studies

For meiotic chromosome counts, unopened floral buds of suitable sizes were fixed in a freshly prepared Carnoy's fixative (mixture of alcohol, chloroform, and glacial acetic acid in a volume ratio 6 : 3 : 1) for 24 h. These were subsequently transferred to 70% alcohol and stored in refrigerator at 4°C until used for meiotic analysis. Meiocytes were prepared by squashing the developing anthers and stained with acetocarmine (1%). Chromosome number was determined at diakinesis/M-I/II/A-I/II from freshly prepared slides with light microscope Olympus. 500−600 pollen mother cells were analyzed for meiotic behaviour at different stages, metaphase-I/II (M-I/II), anaphase-I/II (A-I/II), and telophase-I/II (T-I/II).

2.3. Pollen Grain Analysis

Pollen fertility was estimated through stainability tests using glycerol-acetocarmine (1 : 1) mixture and aniline blue (1%). Up to 450−800 pollen grains were examined for pollen fertility and size frequencies. Well-filled pollen grains with stained nuclei were taken as apparently fertile while shriveled and unstained pollens were counted as sterile.

2.4. Photomicrographs

Photomicrographs from the freshly prepared desirable slides having clear chromosome counts, dyads, triads, tetrads, and pollen grains were taken with a digital imaging system of Leica QWin.

3. Results

3.1. Chromosome Number

Hieracium crocatum Bunge ex. Ledeb. The present species revealed the diploid cytotype (2n = 10, Figure 1(a)), which is a first ever chromosome report for the species.

Figure 1.

Figure 1

Open in a new tab

(a) Hieracium crocatum, PMC showing 5II at M-I. (b-c) H. umbellatum: (b) diploid cytotype, PMC showing 5II at M-I, (c) hexaploid cytotype, PMC showing 27II at diakinesis. (d) Lactuca dissecta, A PMC showing 8II at M-I. (e) L. dolichophylla, PMC showing 8II at M-I. (f) L. lessertiana, PMC showing 8II at M-I. (g) L. macrorhiza, PMC showing 8II at M-I. (h) L. sativa, PMC showing 9II at M-I. (i) L. serriola, PMC showing 9II at M-I. (j) Prenanthes brunoniana, PMC showing 8 bivalents at M-I. (k–m) Taraxacum officinale: (k) diploid cytotype, PMC of diploid cytotype showing 8II at diakinesis, (l) triploid cytotype, PMC showing 5II + 14I at M-I (bivalents with arrow), and (m) tetraploid cytotype, PMC showing 1IV + 13II + 2I (univalents with arrow and quadrivalent with star). (n-o) Tragopogon dubius: (n) diploid, PMC showing 7II at M-I, (o) tetraploid cytotype, PMC showing 14II at diakinesis. (p) T. gracilis, PMC showing 7II at M-I. (q) Youngia glauca, PMC showing 7II at diakinesis, (r) Y. japonica, PMC showing 8II at M-I. (s) Y. tenuifolia, A PMC showing 5II at diakinesis. (M-I: metaphase-I, scale: 10 μm, IV: quadrivalent, II: bivalent, I: univalent.)

H. umbellatum L. Both the cytotypes, 2n = 10 (Figure 1(b)) and 2n = 54 (Figure 1(c)), are the new records from the world, although the species is already known to have 2n = 18 [5] and 2n = 27 [6] from outside India. Polyploid cytotypes show some enlargement in vegetative and floral characters (Table 2).

Table 2.

Morphological comparison of different cytotypes of tribe Cichorieae from Western Himalayas.

Taxon/voucher data Chromosome number (2n) Leaf size (mean ± SD) Shape of leaf Flower colour Average number of capitula/plant Stomatal size (µm) (mean ± SD) Pollen size (µm) (mean ± SD) Stomatal index (%)
Hieracium umbellatum                
P1—52763 10 4.0 ± 0.2 × 1.0 ± 0.3 Entire Yellow 17 23.21 ± 0.4 × 31.54 ± 2.4 16.75 ± 0.7 × 20.1 ± 1.2 33.33
P2—52774 54 7.0 ± 1.2 × 1.5 ± 0.5 Serrate-toothed Yellow 22 29.80 ± 2.1 × 34.26 ± 1.7 15.0 ± 0.56 × 20.0 ± 0.9 23.07
Taraxacum officinale                
P1—52687 16 20.0 ± 0.6 × 1.9 ± 0.9   Yellow 3 17.6 ± 0.4 × 14.5 ± 0.9 18.75 ± 0.4 × 20.62 ± 0.8 28.57
P2—52693 32 5.9 ± 1.2 × 2.6 ± 0.6   Yellow 4 18.75 ± 0.3 × 20.1 ± 0.4 18.98 ± 1.4 × 19.75 ± 1.6 23.07
P3—57491 24 6.9 ± 0.6 × 1.9 ± 0.7   Purple 6 18.30 ± 1.3 × 18.98 ± 2.8 18.75 ± 2.4 × 23.0 ± 2.5 30.0
Tragopogon dubius                
P1—57503 14 15 ± 0.5 × 0.6 ± 0.3 Linear Yellow 23 22.19 ± 0.5 × 27.54 ± 0.7 23.0 ± 1.3 × 12.0 ± 0.8 28.5
P2—57505 28 15 ± 0.5 × 0.9 ± 0.3 Linear lanceolate Yellow 17 18.36 ± 1.2 × 15.25 ± 0.8 22.75 ± 0.7 × 20.11 ± 1.4 16.66
Open in a new tab

*SD: standard deviation.

Lactuca dissecta D. Don. The present report (2n = 16, Figure 1(d)) is in line with many previous reports from India [7–10] and abroad [11].

L. dolichophylla Kitam. The present chromosome report of 2n = 16 (Figure 1(e)) is in line with many previous reports from India [7, 8, 10, 12].

L. lessertiana (Wall. ex DC) C. B. Clarke. The present meiotic studies reveal a diploid cytotype (2n = 16, Figure 1(f)) which is a first ever chromosome report for the species.

L. macrorhiza (Royle) Hook. f. The present chromosome report of 2n = 16 (Figure 1(g)) is already confirmed by many workers [8, 12, 13] from India. There is no chromosome record from abroad for the species.

L. sativa L. Meiotic analysis of the species reveals the diploid cytotype (2n = 18, Figure 1(h)), which is in conformity with the previous works of Chatterjee and Sharma [14] and Gupta and Gill [15].

L. serriola L. The present chromosome report (2n = 18, Figure 1(i)) is confirmed by many workers from India [7, 12, 15, 16].

Prenanthes brunoniana C. B. Clarke. The present chromosome report of 2n = 16 (Figure 1(j)) is in conformity to only previous report from Garhwal, Uttarakhand, by Shetty [8].

Taraxacum officinale L. The present meiotic investigation revealed three cytotypes, 2n = 2x = 16 (Figure 1(k)), 2n = 3x = 24 (Figure 1(l)), and 2n = 4x = 32 (Figure 1(m)). Both diploid and tetraploid cytotypes are common and are reported by many workers [17]. Gupta et al. [13], besides triploid cytotype, also reported some other cytotypes, that is, 2n = 26, 27, 32, 38, and 40. Morphologically, the tetraploids do not show any robust and gigas effect due to polyploidy (Table 2), but they certainly show a lot of variation in shape of leaves.

Tragopogon dubius Scop. Both the cytotypes, 2n = 14 (Figure 1(n)) and 2n = 28 (Figure 1(o)), are varied cytotypes at world level. The species is reported earlier with 2n = 24 by Koul and Gohil [18] and Mehra and Remanandan [12] from Kashmir Himalayas. From outside India, the species is known to have 2n = 12, 24, and 36 [5]. Morphologically, tetraploid cytotype does not show any gigas effect as compared to diploid (Table 2).

T. gracilis D. Don. The present chromosome report (2n = 14, Figure 1(p)) is a varied chromosome count for the species at world level. Earlier, Mehra and Remanandan [12] reported diploid cytotype with 2n = 12 from the Western Himalayas.

Youngia glauca Edgew. The present cytological investigation reveals the diploid cytotype (2n = 14, Figure 1(q)), which is a varied chromosome report. Earlier, there is a maiden cytological report of 2n = 16 [19] from Kinnaur valley.

Y. japonica (L.) DC. The present chromosome report (2n = 16, Figure 1(r)) confirms the earlier reports from different localities of India and abroad [20].

Y. tenuifolia (Wild.) Babcock and Stebbins. This chromosome report of 2n = 10 (Figure 1(s)) is for the first time reported from India. The same number is frequently reported from abroad [21, 22].

3.2. Meiotic Abnormalities

Meiotic abnormalities have been recorded in almost all the studied populations of different species in the form of cytomixis, chromatin stickiness, unoriented bivalent, bridges, laggards, or multipolarity at different stages of meiosis (Tables 3 and 4, Figures 2(a)–2(k)). From the data, triploid cytotype of Taraxacum officinale shows the highest percentage of chromatin transfer from prophase-I to telophase-II (Table 2, Figure 2(a)). Cytomixis usually led to the formation of pollen mother cells (PMCs) with different chromosome numbers and even empty PMCs in some cases (Figure 2(b)), as is evident in hexaploid cytotype of Hieracium umbellatum. Chromatin stickiness (partial or often complete clumping of bivalents) is found in 16.28 per cent of PMCs and unoriented bivalents were seen in 13.79 per cent of PMCs of tetraploid cytotype of T. officinale (Table 3, Figure 2(c)). The present investigation also reveals abnormal meiosis in the form of chromosomal laggards (maximum in triploid cytotype of T. officinale) and bridges (maximum percentage in tetraploid cytotype of H. umbellatum) at anaphase-I/II and telophase-I/II (Figures 2(e)-2(f)). These meiotic abnormalities led to the abnormal microsporogenesis and the formation of heterogeneous sized pollen grains (Table 4, Figures 2(g)–2(k)) and also affect pollen viability (Table 1).

Table 3.

Data on cytomixis and meiotic course in the studied populations of tribe Cichorieae from Western Himalayas.

Accession number Cytomixis Meiotic course
PMCs involved (% age) Number of PMCs involved (%) PMCs with chromosomal stickiness at M-I (%) PMCs with unoriented bivalents at M-I (%) PMC with bridges (at A-I, II/T-I, II) (%) PMCs with laggards (at A-I, II/T-I, II) (%)
52760 0.8 (10/125) 1-2 3.10 (4/129) 7.20 (9/125) 10.08 (12/114) 10.56 (13/123)
52763 3.96 (8/126) 1-2 5.50 (6/109) 2.30 (3/130) 4.31 (6/139) 1.55 (2/129)
52774 11.71 (13/111) 2–4 11.57 (14/121) 13.79 (16/118) 26.95 (38/141) 10.44 (14/134)
52764 2.4 (3/125) 1-2 4.0 (5/125) —/— 4.0 (5/125) —/—
58562 5.4 (6/111) 1-2 3.17 (4/126) 2.70 (3/111) —/— —/—
57504 —/— 0 0.82 (1/121) 1.80 (2/111) 4.50 (5/111) 0.82 (1/121)
52776 —/— 0 1.66 (2/120) —/— —/— —/—
57510 2.77 (4/144) 1-2 3.47 (5/144) 2.0 (3/144) —/— —/—
58534 —/— 0 —/— —/— —/— —/—
58535 1.0 (1/97) 1-2 11.34 (11/97) 6.1 (6/97) 7.21 (7/97) 2.06 (2/97)
52687 4.35 (5/144) 2-3 5.50 (6/109) 8.39 (11/131) 3.84 (8/130) 10.08 (12/114)
52693 1.78 (2/112) 1-2 7.20 (9/125) —/— 3.10 (4/129) 3.84 (8/130)
57491 29.91 (35/117) 2–6 16.28 (21/129) 13.79 (16/116) 12.5 (17/136) 10.44 (14/134)
57503 2.43 (3/123) 1-2 2.30 (3/130) —/— —/— —/—
57505 1.72 (2/160) 1-2 1.37 (2/145) 2.38 (3/126) 1.16 (2/125) 3.25 (4/123)
52768 —/— 0 —/— —/— —/— —/—
52773 0.88 (1/113) 1-2 3.53 (4/113) 1.76 (2/113) 5.30 (6/113) 1.76 (2/113)
57500 —/— 0 —/— —/— —/— —/—
57771 1.61 (2/124) 1-2 1.62 (2/123) —/— —/— —/—
Open in a new tab

Figures in parenthesis denote observed number of abnormal PMCs in the numerator and total PMCs observed in the denominator.

Table 4.

Data on abnormal microsporogenesis on different accession of tribe Cichorieae from Western Himalayas.

Taxon/accession numbers Monads Dyads Triads Tetrads
WM (%) WM (%) WMN (%) WM (%) WMN (%) WM (%) WMN (%)
58533 —/— —/— 0.97 (1/103) —/— 2.91 (3/103) —/— 96.11 (99/103)
52760 2.75 (3/109) 0.91 (1/109) 2.75 (3/109) —/— 2.75 (3/103) —/— 90.82 (99/109)
52763 —/— —/— 1.80 (2/111) 2.70 (3/111) 1.80 (2/111) 3.60 (4/111) 90.09 (100/111)
52774 1.5 (2/130) 0.76 (1/130) 2.30 (3/130) 3.07 (4/130) 2.3 (3/130) 3.8 (5/130) 86.15 (112/130)
52764 —/— —/— 1.6 (2/120) —/— —/— —/— 98.3 (118/120)
58562 —/— —/— —/— —/— —/— —/— 100 (123/123)
57504 —/— 0.95 (1/105) —/— 1.90 (2/105) 2.8 (3/105) 1.90 (2/105) 92.38 (97/105)
52776 —/— —/— 1.66 (2/121) —/— 4.13 (5/121) —/— 95.04 (115/121)
57510 —/— 0.80 (1/124) 1.61 (2/124) —/— 4.03 (5/124) 1.61 (2/124) 91.93 (114/124)
58534 —/— 1.05 (1/95) —/— —/— —/— —/— 99.2 (139/140)
58535 —/— 1.72 (2/116) 4.31 (5/116) 2.58 (3/116) 4.31 (5/116) 5.17 (6/116) 81.89 (95/116)
52687 —/— 1.5 (2/130) 0.76 (1/130) 0.76 (1/130) 2.30 (3/130) 1.5 (2/130) 93.07 (121/130)
52593 —/— —/— 0.8 (1/125) 2.4 (3/125) 0.8 (1/125) 1.6 (2/125) 94.4 (118/125)
57491 2.29 (2/87) 4.59 (4/87) 1.14 (1/87) 2.29 (2/87) 3.44 (3/87) 11.49 (10/87) 72.41 (63/87)
57503 —/— —/— —/— —/— —/— —/— 100 (121/121)
57505 —/— —/— 0.8 (1/125) —/— 1.6 (2/125) 0.8 (125) 96.8 (121/125)
52768 1.66 (2/121) 4.13 (5/121) 0.82 (1/121) 4.13 (5/121) 0.82 (1/121) 0.82 (1/121) 87.60 (106/121)
52773 —/— 0.86 (1/115) 2.60 (3/115) —/— 2.60 (3/115) 1.73 (2/115) 92.17 (106/115)
57500 1.66 (2/121) 0.82 (1/121) 0 0.82 (1/121) 0.82 (1/121) 13.22 (16/121) 82.64 (100/121)
52771 —/— —/— 3.10 (4/129) 0.75 (1/129) 6.97 (9/129) 8.52 (11/129) 80.60 (104/117)
Open in a new tab

Figures in parenthesis denote observed number of abnormal PMCs in the numerator and total number of PMCs observed in denominator; WMN: without micronuclei, WM: with micronuclei.

Figure 2.

Figure 2

Open in a new tab

(a-b) PMC showing transfer of chromatin material in Taraxacum officinale and Hieracium umbellatum, respectively. (c) PMC at metaphase-I showing chromatin stickiness in tetraploid cytotype of T. officinale. (d) PMC showing late disjunction at anaphase-1 (arrowed). (e) PMC at telophase-II showing chromatin bridges (arrowed). (f) PMC at anaphase-I showing chromatin laggards (arrowed) in triploid cytotype of T. officinale. (g) Monad. (h) Diad with micronuclei. (i) Triad with micronucleus. (j) Tetrad with micronucleus. (k) Heterogeneous sized fertile and sterile (arrowed) pollen grains. (Scale: 10 μm, IV: quadrivalent, II: bivalent, I: univalent.)

4. Discussion

4.1. Chromosome Number

4.1.1. Tribe Cichorieae

The ancestral basic numbers in the tribe are x = 4, 5, and 9, as suggested by Turner et al. [23]. But Stebbins et al. [24] proposed x = 9 as the base number for Cichorieae (Asteraceae in general), which is supported by Tomb et al. [25], with the other numbers (x = 3–9) derived through phylogenetic reduction through chromosomal aberrations, particularly translocation.

Hieracium L. About 360 species are cytologically known with 94 species being diploid and 152 triploid and 149 species are tetraploid and rarely possess aneuploidy (7 spp.). The genus forms agamic complex and is considered monobasic on x = 9. But, the present study reveals another cytotype (2n = 10), suggesting a new base number (x = 5) in the genus.

Lactuca L. A total of 150 species are known taxonomically, of which chromosome numbers for 87 species overall and 14 species from India are known. The chromosome number in the genus varies in the range of 2n = 10–48 and is polybasic on x = 5, 8, 9, and 17, of which x = 9 is the most dominant number.

Prenanthes L. Twenty-two species in the genus are known cytotaxonomically, including 1 from India. The most common base number is x = 8 represented with 19 species, including diploids (16 species) and tetraploids (3 species). However, the intraspecific polyploids are not available in the genus. Besides, x = 9 is also present in 3 species that are diploid. Hence, the genus is proposed to be dibasic on x = 8 and 9.

Taraxacum L. The genus is very complex, reinforcing the reason of having 347 cytologically (including 10 species from India) worked-out species. The chromosome numbers vary in the range of 2n = 8–64, the most common of which is 2n = 3x = 24 (230 spp.) on x = 8, followed by diploid (47 spp.) on the same base number. Genus is reported to have a series of base numbers on x = 4, 6, 8, 9, and 11, but only x = 8 is known to have well-developed polyploid races (2x–6x). Intraspecific polyploidy is also known to occur in x = 9 and 11 (1 species each).

Tragopogon L. 75 species in the genus are cytologically known, with chromosome number in the range of 2n = 12–36, almost all based on x = 6. The overall polyploidy in the genus is 26.6% (20 spp.), out of which 14 species show intraspecific polyploidy. The variable chromosome number of 2n = 14 is found in 4 species (including the present data) and 2n = 28 in only one species (from the present data).

Youngia L. A total of 35 species are taxonomically known, cytology is reported for only 14 species (including 5 from India), with 9 species showing polyploid cytotypes (3x, 4x, and 6x). The chromosome numbers reported so far are 2n = 10, 15, 16, 20, 24, 32, and 42, out of which 2n = 10 (43.7%) is the most common followed by 2n = 16 (31.2%). The genus is polybasic (x = 5, 7, and 8), of which x = 5 is most common.

4.2. Meiotic Abnormality

The phenomenon of inter-PMC migration of chromatin/chromosome between/among the contiguous meiocytes through cytomictic channels is termed as cytomixis (coined by Gates [26]). However, the phenomenon has been reported for the first time in gymnosperms by Arnoldy [27] and subsequently in angiosperms by Koernicke [28]. Since that time, cytomixis has been reported in a large number of plants [29]. Transfer of chromatin or chromosomes may take place through such inter-PMC cytomictic channels [30–32]. Some workers reported cytomixis to be more prevalent in polyploids than their diploid counterparts [33, 34]. Occasionally, either hypoploid meiocytes [35–37] or enucleated meiocytes or meiocytes with a hyperploid number of chromosomes have been reported [30, 37–39]. It is very much clear that the enucleated meiocytes die, but hypo- and hyperploid meiocytes could lead to the formation of gametes with variable chromosome number and size. Cytomixis is considered as a process of evolutionary significance because it results in change in gametic chromosome numbers [30, 40]. Chromosome stickiness also results in the formation of fragmented chromatin. This chromatin stickiness, late or nondisjuncting bivalents, and chromosomal laggards seem to be responsible for chromosomal bridges [41]. All these meiotic abnormalities consequently assert an effect on microsporogenesis, leading to the formation of monads, dyads, triads, or polyads with or without micronuclei, which ultimately produce heterogeneous sized (large and small) fertile pollen grains and reduced pollen fertility. The size difference may be due to the formation of unreduced gametes (2n), which may produce plants with higher ploidal level through polyploidization (for review, see [42–45]).

As observed in the presently investigated data, the chromatin rearrangement due to meiotic abnormalities is considered the base of inter- or intraspecific diversity. Further, it provides a catalogue for studying different evolutionary trends such as breeding system or polyploidy and hybridization.

Acknowledgments

The authors are grateful to the University Grants Commission of New Delhi (DRS SAP III) and I.P.L.S. (DBT) for providing financial assistance. The authors are highly thankful to the Head, Department of Botany, Punjabi University, Patiala, for providing necessary lab facilities during the work.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

References

  • 1.Shi Z., Ge X. J., Kilian N., et al. Cichorieae. In: Wu Z. Y., Raven P. H., Hong D. Y., editors. Flora of China. 20-21. St. Louis, Mo, USA: Science Press, Beijing, China, Missouri Botanical Garden Press; 2011. pp. 195–353. (Asteraceae). [Google Scholar]
  • 2.Howard M. Traditional Folk Remedies. London, UK: Ebury Press; 1987. [Google Scholar]
  • 3.Clare B. A., Conroy R. S., Spelman K. The diuretic effect in human subjects of an extract of Taraxacum officinale folium over a single day. Journal of Alternative and Complementary Medicine. 2009;15(8):929–934. doi: 10.1089/acm.2008.0152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Singh K. N. Traditional knowledge on ethnobotanical uses of plant biodiversity: a detailed study from the Indian western Himalaya. Biodiversity: Research and Conservation. 2012;28:63–67. doi: 10.2478/v10119-012-0028-z. [DOI] [Google Scholar]
  • 5.Nazarova E. A. Chromosome numbers in the Caucasian representatives of the families Asteraceae, Brassicaceae, Fabaceae, Limoniaceae. Botanicheskii Zhurnal. 1984;69:972–975. (Rus). [Google Scholar]
  • 6.Volkova S. A., Boyko E. V. Chromosome numbers in some species of Asteraceae from the southern part of the Soviet Far East. Botanicheskii Zhurnal. 1986;71:1693. [Google Scholar]
  • 7.Mehra P. N., Gill B. S., Mehta J. K., Sidhu S. S. Cytological investigations on the Indian Compositae. I. North-Indian taxa. Caryologia. 1965;18:35–68. [Google Scholar]
  • 8.Shetty B. V. IOPB chromosome number reports XIV. Taxon. 1967;16:552–571. [Google Scholar]
  • 9.Gupta R. C., Himshikha, Kumar P., Dhaliwal R. S. Cytological studies in some plants from cold deserts of India, Lahaul and Spiti (Himachal Pradesh) Chromosome Botany. 2009;4(1):5–11. doi: 10.3199/iscb.4.5. [DOI] [Google Scholar]
  • 10.Bala S., Gupta R. C. IAPT/IOPB chromosome data 12. In: Marhold K., editor. Taxon. Vol. 60. 2011. pp. 1784–1786. [Google Scholar]
  • 11.Razaq Z. A., Vahidy A. A., Ali S. I. Chromosome numbers in Compositae from Pakistan. Annals of the Missouri Botanical Garden. 1994;81(4):800–808. doi: 10.2307/2399925. [DOI] [Google Scholar]
  • 12.Mehra P. N., Remanandan P. IOPB chromosome number reports XXII. Taxon. 1969;18:433–442. [Google Scholar]
  • 13.Gupta R. C., Gill B. S., Garg R. K. Chromosomal conspectus of Western Himalayan Compositae. Aspects of Plant Sciences. 1989;11:427–437. [Google Scholar]
  • 14.Chatterjee T., Sharma A. K. Cytotaxonomy of cichorieae. Genetica. 1969;40(1):577–590. doi: 10.1007/BF01787382. [DOI] [Google Scholar]
  • 15.Gupta R. C., Gill B. S. Cytology of family Compositae of Punjab plains. Proceedings of the National Academy of Sciences, India, Section B. 1983;49:359–370. [Google Scholar]
  • 16.Koul A. K., Wakhlu A., Karihaloo J. L. Chromosome numbers of some flowering plants of Jammu (Western Himalayas) II. Chromosome Information Service (CIS) 1976;(20)
  • 17.Gill B. S., Gupta R. C. Cytological investigations on the North-West Himalayan Taraxacum officinale complex (Compositae) In: Bir S. S., editor. Recent Researches in Plant Sciences. New Delhi, India: Kalyani Publishers; 1979. pp. 292–301. [Google Scholar]
  • 18.Koul A. K., Gohil P. N. Cytotaxonomical conspectus of the flora of Kashmir, I. Chromosome numbers of some common plants. Phyton. 1973;15:57–66. [Google Scholar]
  • 19.Kaur D., Singhal V. K., Marhold K. IAPT/IOPB chromosome data 13. Taxon. 2013;61:889–902. [Google Scholar]
  • 20.Gupta R. C., Kataria V., Mehra A. Cytomorphological studies in some gamopetalous species of Western Himalaya: an attempt to add new or varied cytotypes. Chromosome Botany. 2012;7:59–65. [Google Scholar]
  • 21.Stepanov N. V. Chromosome numbers of some higher plants taxa of the flora of Krasnoyarsk region. Botanicheskii Zhurnal. 1994;79:135–139. [Google Scholar]
  • 22.Rudyka E. G. Chromosome numbers in vascular plants from the southern part of the Russian Far East. Botaničeskij Žhurnal (Moscow & Leningrad) 1995;80:87–90. [Google Scholar]
  • 23.Turner B. L., Ellis W. L., King R. M. Chromosome numbers in the Compositae IV. American Journal of Botany. 1961;48(3):216–223. doi: 10.2307/2439399. [DOI] [Google Scholar]
  • 24.Stebbins G. L., Jenkins J. K., Walters M. S. Chromosomes and Phylogeny in the Compositae, Tribe Cichorieae. Vol. 16. Berkeley, Calif, USA: University of California Publications in Botany; 1953. [Google Scholar]
  • 25.Tomb A. S., Chambers K. L., Kyhos D. W., Powell A. M., Raven P. H. Chromosome numbers in the Compositae. XIV. Lactuceae. The American Journal of Botany. 1978;65:717–722. doi: 10.2307/2442146. [DOI] [Google Scholar]
  • 26.Gates R. R. Pollen formation in oenothera gigas. Annals of Botany. 1911;25(4):909–940. [Google Scholar]
  • 27.Arnoldy W. Beiträge zur Morphologie der Gymnospermen. IV. Was sind die “Keimbläschen” oder “Hofmeisters-Körperchen” in der Eizelle der Abietineen? Flora. 1900;87:194–204. [Google Scholar]
  • 28.Koernicke M. Uber ortsveranderung von Zellkarnern S B Niederhein Ges Natur-U Heilkunde Bonn A. 1901. [Google Scholar]
  • 29.Singhal V. K., Kumar P. Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeata Royle) Journal of Biosciences. 2008;33(3):371–380. doi: 10.1007/s12038-008-0057-0. [DOI] [PubMed] [Google Scholar]
  • 30.Falistocco E., Tosti N., Falcinelli M. Cytomixis in pollen mother cells of diploid Dactylis, one of the origins of 2n gametes. Journal of Heredity. 1995;86(6):448–453. [Google Scholar]
  • 31.Mursalimov S. R., Deineko E. V. An ultrastructural study of cytomixis in tobacco pollen mother cells. Protoplasma. 2011;248(4):717–724. doi: 10.1007/s00709-010-0234-5. [DOI] [PubMed] [Google Scholar]
  • 32.Kravets E. A. Nature, significance, and cytological consequences of cytomixis. Cytology and Genetics. 2012;46(3):188–195. doi: 10.3103/S0095452712030061. [DOI] [PubMed] [Google Scholar]
  • 33.Semyarkhina S. Y. A., Kuptsou M. S. Cytomixis in various forms of sugar beet. Vestsi AN BSSR Ser Biyal. 1974;(4):43–47. [Google Scholar]
  • 34.Singhal V. K., Gill B. S., Dhaliwal R. S. Status of chromosomal diversity in the hardwood tree species of Punjab state. Journal Cytology and Genetics. 2007;8:67–83. [Google Scholar]
  • 35.Ashraf M., Gohil R. N. Cytology of legumes of Kashmir Himalaya. V. Cytomixis and chromosome migration in Astragalus subuliformis DC. Nucleus. 1994;37:119–122. [Google Scholar]
  • 36.Morikawa T., Leggett J. M. Cytological and morphological variations in wild populations of Avena canariensis from the Canary Islands. Genes & Genetic Systems. 1996;71(1):15–21. doi: 10.1266/ggs.71.15. [DOI] [Google Scholar]
  • 37.Sheidai M., Jafari S., Taleban P., Keshavarzi M. Cytomixis and unreduced pollen grain formation in alopecurus L. and catbrosa beauv. (Poaceae) Cytologia. 2009;74(1):31–41. doi: 10.1508/cytologia.74.31. [DOI] [Google Scholar]
  • 38.Bellucci M., Roscini C., Mariani A. Cytomixis in pollen mother cells of Medicago sativa L. Journal of Heredity. 2003;94(6):512–516. doi: 10.1093/jhered/esg096. [DOI] [PubMed] [Google Scholar]
  • 39.Kumar P., Singhal V. K., Kaur D. mpaired male meiosis due to irregular synapsis coupled with cytomixis in a new diploid cytotype of Dianthus angulatus (Caryophyllaceae) from Indian cold deserts. Folia Geobotanica. 2012;47(1):59–68. doi: 10.1007/s12224-011-9107-8. [DOI] [Google Scholar]
  • 40.Liu Y., Hui R.-K., Deng R.-N., Wang J.-J., Wang M., Li Z.-Y. Abnormal male meiosis explains pollen sterility in the polyploid medicinal plant Pinellia ternata (Araceae) Genetics and Molecular Research. 2012;11(1):112–120. doi: 10.4238/2012.January.17.1. [DOI] [PubMed] [Google Scholar]
  • 41.Kumar S., Jeelani S. M., Rani S., Gupta R. C., Kumari S. Cytology of five species of subfamily Papaveroideae from the Western Himalayas. Protoplasma. 2013;250(1):307–316. doi: 10.1007/s00709-012-0413-7. [DOI] [PubMed] [Google Scholar]
  • 42.Villeux R. Diploid and polyploid gametes in crop plants: mechanisms of formation and utilization in plant breeding. In: Janick J., editor. Plant Breeding Reviews. Connecticut, Conn, USA: AVI Publishing Co. Wesport; 1985. p. 442. [Google Scholar]
  • 43.Bretagnolle F., Thomson J. D. Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants. New Phytologist. 1995;129(1):1–22. doi: 10.1111/j.1469-8137.1995.tb03005.x. [DOI] [PubMed] [Google Scholar]
  • 44.Fatemeh F., Sheidai M., Asadi M. Cytological study the genus Arenaria L. (Caryophyllaceae) Caryologia. 2010;63(2):149–156. doi: 10.1080/00087114.2010.589720. [DOI] [Google Scholar]
  • 45.Jeelani S. M., Rani S., Kumar S., Kumari S., Gupta R. C. Meiotic studies in some members of Caryophyllaceae juss. from the western Himalayas. Acta Biologica Cracoviensia Series Botanica. 2011;53(1):86–95. doi: 10.2478/v10182-011-0012-5. [DOI] [Google Scholar]

Articles from The Scientific World Journal are provided here courtesy of Wiley

RESOURCES