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Collagen formation assay of osteoblasts. (A) Photomicrographs stained with Sirius Red dye showing increased formation of collagen matrix of osteoblasts treated with increasing concentrations (10–100 μg/ml) of PG extract at 48 h (B) Quantitative data of collagen content expressed in the form of percent collagen content respective to control. Data represented as mean ± SEM of three independent experiments. **p < 0.01 and ***p < 0.001 as compared with control.
