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(A) The SERS electropherogram of the three aromatic side chain amino acids indicates that Trp migrates at tm = 224 ± 6 s, Phe at tm = 310 ± 11 s, and Tyr at tm = 361 ± 8 s. The signal threshold (>4σ) was set to 250 counts. (B) Averaged SERS spectra of (i) Trp, (ii) Phe, and (iii) Tyr were extracted from the electropherogram in (A) between 223.5 and 224.5 s, 312.3 and 313.1 s, and 361.1 and 362.1 s, respectively. The amino acid concentration in this mixture is 3.3×10−4 M.
