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. 1984 Jan;73(1):224–230. doi: 10.1172/JCI111195

Characterization of a virus that causes transient aplastic crisis.

N S Young, P P Mortimer, J G Moore, R K Humphries
PMCID: PMC425004  PMID: 6317715

Abstract

Transient aplastic crisis in children with congenital hemolytic anemias has been linked epidemiologically to infection with a serum parvovirus-like virus (SPLV). The virus is found in the blood in the early stages of the crisis, and serum containing SPLV inhibits erythroid colony formation in vitro. After sedimentation of virus-containing sera through a sucrose density gradient, colony inhibitory activity is present in the particulate fraction and separate from serum immunoglobulins. No inhibitory activity can be recovered from convalescent-phase sera after similar fractionation procedures. Inhibition of erythroid colony formation in vitro is not a feature of sera from other viral infections. The pattern of resistance of SPLV activity to chemicals and enzymes is compatible with it being a parvovirus. By using replating techniques, a target of SPLV has been identified as a late erythroid progenitor cell. Neither SPLV antigen nor anti-SPLV IgM was present in the sera of patients with other forms of bone marrow failure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. J., Davis L. R., Jones S. E., Pattison J. R., Serjeant G. R. The development and use of an antibody capture radioimmunoassay for specific IgM to a human parvovirus-like agent. J Hyg (Lond) 1982 Apr;88(2):309–324. doi: 10.1017/s0022172400070169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aye M. T., Niho Y., Till J. E., McCulloch E. A. Studies of leukemic cell populations in culture. Blood. 1974 Aug;44(2):205–219. [PubMed] [Google Scholar]
  3. BIERMAN H. R., NELSON E. R. HEMATODEPRESSIVE VIRUS DISEASES OF THAILAND. Ann Intern Med. 1965 May;62:867–884. doi: 10.7326/0003-4819-62-5-867. [DOI] [PubMed] [Google Scholar]
  4. Bukrinskaya A. G. Penetration of viral genetic material into host cell. Adv Virus Res. 1982;27:141–204. doi: 10.1016/s0065-3527(08)60435-2. [DOI] [PubMed] [Google Scholar]
  5. Cashman J., Henkelman D., Humphries K., Eaves C., Eaves A. Individual BFU-E in polycythemia vera produce both erythropoietin dependent and independent progeny. Blood. 1983 May;61(5):876–884. [PubMed] [Google Scholar]
  6. Cohen B. J., Mortimer P. P., Pereira M. S. Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV). J Hyg (Lond) 1983 Aug;91(1):113–130. doi: 10.1017/s0022172400060095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cossart Y. E., Field A. M., Cant B., Widdows D. Parvovirus-like particles in human sera. Lancet. 1975 Jan 11;1(7898):72–73. doi: 10.1016/s0140-6736(75)91074-0. [DOI] [PubMed] [Google Scholar]
  8. Engers H. D., Louis J. A., Zubler R. H., Hirt B. Inhibition of T cell-mediated functions by MVM(i), a parvovirus closely related to minute virus of mice. J Immunol. 1981 Dec;127(6):2280–2285. [PubMed] [Google Scholar]
  9. GASSER C. Aplasia of erythropoiesis; acute and chronic erythroblastopenias or pure (red cell) aplastic anaemias in childhood. Pediatr Clin North Am. 1957 May;:445–468. doi: 10.1016/s0031-3955(16)30511-9. [DOI] [PubMed] [Google Scholar]
  10. GINSBERG H. S. Characteristics of the new respiratory viruses (adenoviruses). II. Stability to temperature and pH alterations. Proc Soc Exp Biol Med. 1956 Oct;93(1):48–52. doi: 10.3181/00379727-93-22659. [DOI] [PubMed] [Google Scholar]
  11. Gregory C. J., Eaves A. C. Human marrow cells capable of erythropoietic differentiation in vitro: definition of three erythroid colony responses. Blood. 1977 Jun;49(6):855–864. [PubMed] [Google Scholar]
  12. Hagler L., Pastore R. A., Bergin J. J., Wrensch M. R. Aplastic anemia following viral hepatitis: report of two fatal cases and literature review. Medicine (Baltimore) 1975 Mar;54(2):139–164. [PubMed] [Google Scholar]
  13. Kelleher J. F., Luban N. L., Mortimer P. P., Kamimura T. Human serum "parvovirus": a specific cause of aplastic crisis in children with hereditary spherocytosis. J Pediatr. 1983 May;102(5):720–722. doi: 10.1016/s0022-3476(83)80243-1. [DOI] [PubMed] [Google Scholar]
  14. Mortimer P. P., Humphries R. K., Moore J. G., Purcell R. H., Young N. S. A human parvovirus-like virus inhibits haematopoietic colony formation in vitro. 1983 Mar 31-Apr 6Nature. 302(5907):426–429. doi: 10.1038/302426a0. [DOI] [PubMed] [Google Scholar]
  15. Ortega J. A., Ma A., Shore N. A., Dukes P. P., Merigan T. C. Suppressive effect of interferon on erythroid cell proliferation. Exp Hematol. 1979 Mar;7(3):145–150. [PubMed] [Google Scholar]
  16. Pardoll D. M., Rodeheffer R. J., Smith R. R., Charache S. Aplastic crisis due to extensive bone marrow necrosis in sickle cell disease. Arch Intern Med. 1982 Nov;142(12):2223–2225. [PubMed] [Google Scholar]
  17. Pattison J. R., Jones S. E., Hodgson J., Davis L. R., White J. M., Stroud C. E., Murtaza L. Parvovirus infections and hypoplastic crisis in sickle-cell anaemia. Lancet. 1981 Mar 21;1(8221):664–665. doi: 10.1016/s0140-6736(81)91579-8. [DOI] [PubMed] [Google Scholar]
  18. Paul P. S., Mengeling W. L., Brown T. T., Jr Replication of porcine parvovirus in peripheral blood lymphocytes, monocytes, and peritoneal macrophages. Infect Immun. 1979 Sep;25(3):1003–1007. doi: 10.1128/iai.25.3.1003-1007.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Quesenberry P., Levin J., Zuckerman K., Rencricca N., Sullivan R., Tyler W. Stem cell migration induced by erythropoietin or haemolytic anaemia: the effects of actinomycin and endotoxin contamination of erythropoietin preparations. Br J Haematol. 1979 Feb;41(2):253–269. doi: 10.1111/j.1365-2141.1979.tb05854.x. [DOI] [PubMed] [Google Scholar]
  20. Serjeant G. R., Topley J. M., Mason K., Serjeant B. E., Pattison J. R., Jones S. E., Mohamed R. Outbreak of aplastic crises in sickle cell anaemia associated with parvovirus-like agent. Lancet. 1981 Sep 19;2(8247):595–597. doi: 10.1016/s0140-6736(81)92739-2. [DOI] [PubMed] [Google Scholar]
  21. Shadduck R. K., Winkelstein A., Zeigler Z., Lichter J., Goldstein M., Michaels M., Rabin B. Aplastic anemia following infectious mononucleosis: possible immune etiology. Exp Hematol. 1979 May;7(5):264–271. [PubMed] [Google Scholar]
  22. WALT F., TAYLOR J. E., MAGILL F. B., NESTADT A. Erythroid hypoplasia in kwashiorkor. Br Med J. 1962 Jan 13;1(5271):73–75. doi: 10.1136/bmj.1.5271.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van der Sar A. Aplastic sickle cell crisis: a report on four cases. Trop Geogr Med. 1967 Dec;19(4):273–285. [PubMed] [Google Scholar]

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