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. 1984 Jan;73(1):231–240. doi: 10.1172/JCI111196

Utilization of individual lecithins in intestinal lipoprotein formation in the rat.

G M Patton, S B Clark, J M Fasulo, S J Robins
PMCID: PMC425005  PMID: 6690480

Abstract

To determine the molecular species composition of lecithins of different nascent lipoproteins, high density lipoproteins (HDL), very low density lipoproteins (VLDL), and chylomicrons (CM) were isolated from the mesenteric lymph of rats. Lymph was collected at 0 degrees C with 5,5'-dithiobis-2-dinitrobenzoic acid added to inhibit lecithin-cholesterol acyl transferase. CM were separated by ultracentrifugation and HDL from VLDL by dextran SO4-MG+2 precipitation. Molecular species of lecithin were directly isolated by reverse phase high performance liquid chromatography. In fasted animals, the lecithin compositions of lymph HDL and VLDL were virtually the same and closely resembled the lecithin composition of intestinal mucosa. When bile lecithin was eliminated (by bile diversion), there was a marked change in lecithin composition of all lipoprotein and mucosal samples, which was most notable for a reduction in 16:0-species (which are predominant in bile) and a relative increase in the corresponding 18:0-species. Feeding unsaturated triglycerides (triolein, trilinolein, or a combination of triolein and trilinolein) also resulted in a change in HDL and VLDL lecithin composition. The effect was similar whether bile lecithin was present or eliminated and was notable for a reduction in 16:0-species, an increase in 18:0-species, and the emergence of large amounts of diunsaturated lecithins that corresponded to the fatty acid composition of the triglycerides fed (i.e., 18:1-18:1, 18:2-18:2, and 18:1-18:2 lecithins). When bile-diverted rats were infused via the duodenum with a mix of [14C]choline-labeled lecithins (isolated from the bile of other rats), the incorporation of infused lecithins into different lymph lipoproteins was distinctly different. Individual lecithins were incorporated to a variable extent into each lipoprotein. In fasted rats the specific activities of all major molecular species of lecithin were relatively greater in VLDL than HDL, indicating that HDL derived proportionately more of its lecithins from an endogenous pool than did VLDL. Feeding triolein changed the specific activities of more of the lecithin species of VLDL than of HDL. The specific activities of lecithins in CM were more similar to VLDL than to HDL after triolein feeding. Results thus indicate that, although the lecithins of different mesenteric lymph lipoproteins are similar and may be derived from membrane sites with the same lecithin composition, lecithins incorporated into different lipoproteins originate from different metabolic pools and/or by different mechanisms.

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Selected References

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  1. Alpers D. H., Lancaster N., Schonfeld G. The effects of fat feeding on apolipoprotein AI secretion from rat small intestinal epithelium. Metabolism. 1982 Aug;31(8):784–790. doi: 10.1016/0026-0495(82)90076-2. [DOI] [PubMed] [Google Scholar]
  2. Arvidson G. A. Biosynthesis of phosphatidylcholines in rat liver. Eur J Biochem. 1968 Aug;5(3):415–421. doi: 10.1111/j.1432-1033.1968.tb00385.x. [DOI] [PubMed] [Google Scholar]
  3. Arvidson G. A., Nilsson A. Formation of lymph chylomicron phosphatidylcholines in the rat during the absorption of safflower oil or triolein. Lipids. 1972 May;7(5):344–348. doi: 10.1007/BF02532653. [DOI] [PubMed] [Google Scholar]
  4. Bartholow L. C., Geyer R. P. Sterol efflux from mammalian cells induced by human serum albumin-phospholipid complexes. Dependence on phospholipid acyl chain length, degree of saturation, and net charge. J Biol Chem. 1982 Mar 25;257(6):3126–3130. [PubMed] [Google Scholar]
  5. Eisenberg S. Plasma lipoprotein conversions: the origins of low-density and high-density lipoproteins. Ann N Y Acad Sci. 1980;348:30–47. doi: 10.1111/j.1749-6632.1980.tb21289.x. [DOI] [PubMed] [Google Scholar]
  6. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  7. Gordon D. T., Pitas R. E., Jensen R. G. Effects of diet and type IIa hyperlipoproteinemia upon structure of triacylglycerols and phosphatidyl cholines from human plasma lipoproteins. Lipids. 1975 May;10(5):270–283. doi: 10.1007/BF02532700. [DOI] [PubMed] [Google Scholar]
  8. Green P. H., Glickman R. M. Intestinal lipoprotein metabolism. J Lipid Res. 1981 Nov;22(8):1153–1173. [PubMed] [Google Scholar]
  9. Green P. H., Tall A. R., Glickman R. M. Rat intestine secretes discoid high density lipoprotein. J Clin Invest. 1978 Feb;61(2):528–534. doi: 10.1172/JCI108963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Imaizumi K., Havel R. J., Fainaru M., Vigne J. L. Origin and transport of the A-I and arginine-rich apolipoproteins in mesenteric lymph of rats. J Lipid Res. 1978 Nov;19(8):1038–1046. [PubMed] [Google Scholar]
  11. Jackson R. L., Gotto A. M., Stein O., Stein Y. A comparative study on the removal of cellular lipids from Landschütz ascites cells by human plasma apolipoproteins. J Biol Chem. 1975 Sep 25;250(18):7204–7209. [PubMed] [Google Scholar]
  12. Kostner G. M. Letter: Enzymatic determination of cholesterol in high-density lipoprotein fractions prepared by polyanion precipitation. Clin Chem. 1976 May;22(5):695–695. [PubMed] [Google Scholar]
  13. Mansbach C. M., 2nd The origin of chylomicron phosphatidylcholine in the rat. J Clin Invest. 1977 Aug;60(2):411–420. doi: 10.1172/JCI108790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morgan R. G. The effect of operation and the method of feeding on the lymphatic transport of fat by bile fistula rats. Q J Exp Physiol Cogn Med Sci. 1966 Jan;51(1):33–41. doi: 10.1113/expphysiol.1966.sp001822. [DOI] [PubMed] [Google Scholar]
  15. Myher J. J., Kuksis A., Shepherd J., Packard C. J., Morrisett J. D., Taunton O. D., Gotto A. M. Effect of saturated and unsaturated fat diets on molecular species of phosphatidylcholine and sphingomyelin of human plasma lipoproteins. Biochim Biophys Acta. 1981 Oct 23;666(1):110–119. doi: 10.1016/0005-2760(81)90096-5. [DOI] [PubMed] [Google Scholar]
  16. Ockner R. K., Hughes F. B., Isselbacher K. J. Very low density lipoproteins in intestinal lymph: role in triglyceride and cholesterol transport during fat absorption. J Clin Invest. 1969 Dec;48(12):2367–2373. doi: 10.1172/JCI106203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parthasarathy S., Subbaiah P. V., Ganguly J. The mechanism of intestinal absorption of phosphatidylcholine in rats. Biochem J. 1974 Jun;140(3):503–508. doi: 10.1042/bj1400503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Patton G. M., Fasulo J. M., Robins S. J. Separation of phospholipids and individual molecular species of phospholipids by high-performance liquid chromatography. J Lipid Res. 1982 Jan;23(1):190–196. [PubMed] [Google Scholar]
  19. Robins S. J. Recirculation and reutilization of micellar bile lecithin. Am J Physiol. 1975 Sep;229(3):598–602. doi: 10.1152/ajplegacy.1975.229.3.598. [DOI] [PubMed] [Google Scholar]
  20. Scow R. O., Stein Y., Stein O. Incorporation of dietary lecithin and lysolecithin into lymph chylomicrons in the rat. J Biol Chem. 1967 Nov 10;242(21):4919–4924. [PubMed] [Google Scholar]
  21. Skipski V. P., Barclay M., Barclay R. K., Fetzer V. A., Good J. J., Archibald F. M. Lipid composition of human serum lipoproteins. Biochem J. 1967 Aug;104(2):340–352. doi: 10.1042/bj1040340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sundler R., Akesson B. Biosynthesis of phosphatidylethanolamines and phosphatidylcholines from ethanolamine and choline in rat liver. Biochem J. 1975 Feb;146(2):309–315. doi: 10.1042/bj1460309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tall A. R., Blum C. B., Forester G. P., Nelson C. A. Changes in the distribution and composition of plasma high density lipoproteins after ingestion of fat. J Biol Chem. 1982 Jan 10;257(1):198–207. [PubMed] [Google Scholar]
  24. Trewhella M. A., Collins F. D. Pathways of phosphatidylcholine biosynthesis in rat liver. Biochim Biophys Acta. 1973 Jan 19;296(1):51–61. doi: 10.1016/0005-2760(73)90042-8. [DOI] [PubMed] [Google Scholar]
  25. Tso P., Balint J. A., Bishop M. B., Rodgers J. B. Acute inhibition of intestinal lipid transport by Pluronic L-81 in the rat. Am J Physiol. 1981 Dec;241(6):G487–G497. doi: 10.1152/ajpgi.1981.241.6.G487. [DOI] [PubMed] [Google Scholar]
  26. Tso P., Lam J., Simmonds W. J. The importance of the lysophosphatidylcholine and choline moiety of bile phosphatidylcholine in lymphatic transport of fat. Biochim Biophys Acta. 1978 Mar 30;528(3):364–372. doi: 10.1016/0005-2760(78)90025-5. [DOI] [PubMed] [Google Scholar]
  27. WHYTE M., GOODMAN D. S., KARMEN A. FATTY ACID ESTERIFICATION AND CHYLOMICRON FORMATION DURING FAT ABSORPTION IN RAT. 3. POSITIONAL RELATIONS IN TRIGLYCERIDES AND LECITHIN. J Lipid Res. 1965 Apr;6:233–240. [PubMed] [Google Scholar]
  28. Warshaw A. L. A simplified method of cannulating the intestinal lymphatic of the rat. Gut. 1972 Jan;13(1):66–67. doi: 10.1136/gut.13.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Windmueller H. G., Wu A. L. Biosynthesis of plasma apolipoproteins by rat small intestine without dietary or biliary fat. J Biol Chem. 1981 Mar 25;256(6):3012–3016. [PubMed] [Google Scholar]
  30. Wu A. L., Clark S. B., Holt P. R. Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo. J Lipid Res. 1975 Jul;16(4):251–257. [PubMed] [Google Scholar]
  31. Wu A. L., Windmueller H. G. Relative contributions by liver and intestine to individual plasma apolipoproteins in the rat. J Biol Chem. 1979 Aug 10;254(15):7316–7322. [PubMed] [Google Scholar]
  32. Zilversmit D. B. The composition and structure of lymph chylomicrons in dog, rat, and man. J Clin Invest. 1965 Oct;44(10):1610–1622. doi: 10.1172/JCI105267. [DOI] [PMC free article] [PubMed] [Google Scholar]

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