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. 1984 Feb;73(2):477–488. doi: 10.1172/JCI111234

Static and dynamic rigidities of normal and sickle erythrocytes. Major influence of cell hemoglobin concentration.

E Evans, N Mohandas, A Leung
PMCID: PMC425039  PMID: 6699172

Abstract

Static and dynamic deformabilities of erythrocytes are important determinants of microcirculatory blood flow. To determine the influence of increased cellular hemoglobin concentration on these properties, we quantitated static and dynamic deformabilities of isolated subpopulations of oxygenated normal and sickle erythrocytes with defined cell densities using micromechanical manipulations of individual cells. The rheological properties measured to characterize static deformability were membrane extensional rigidity and bending rigidity. To characterize dynamic deformability of the cells, we measured the time constants for rapid elastic recovery from extensional and bending deformations. The extensional rigidity of sickle cells increased with increasing cell hemoglobin concentration while that of normal cells was independent of the state of cell hydration. Moreover, sickle cells were found to exhibit inelastic behavior at much lower cell hemoglobin concentrations than normal cells. In contrast, the dynamic rigidity of both normal and sickle cells was increased to the same extent at elevated hemoglobin concentrations. Moreover, this increase in dynamic rigidity with increasing cellular dehydration was much more pronounced than that seen for static rigidity. Both the increased static and dynamic rigidities of the dehydrated sickle cells could be greatly improved by hydrating the cells. This suggests that increased bulk hemoglobin concentration, which is perhaps inordinately increased adjacent to the membrane, plays a major role in regulating the rigidity of sickle cells. In addition, irreversible membrane changes also appear to accompany cell dehydration in vivo, resulting in increased membrane shear rigidity and plastic flow. We expect that the marked increases in rigidity of dehydrated sickle cells observed here may have a major influence on the dynamics of their circulation in the microvasculature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chien S., Sung K. L., Skalak R., Usami S., Tözeren A. Theoretical and experimental studies on viscoelastic properties of erythrocyte membrane. Biophys J. 1978 Nov;24(2):463–487. doi: 10.1016/S0006-3495(78)85395-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chien S., Usami S., Bertles J. F. Abnormal rheology of oxygenated blood in sickle cell anemia. J Clin Invest. 1970 Apr;49(4):623–634. doi: 10.1172/JCI106273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark M. R., Guatelli J. C., White A. T., Shohet S. B. Study on the dehydrating effect of the red cell Na+/K+-pump in nystatin-treated cells with varying Na+ and water contents. Biochim Biophys Acta. 1981 Sep 7;646(3):422–432. doi: 10.1016/0005-2736(81)90311-4. [DOI] [PubMed] [Google Scholar]
  4. Clark M. R., Mohandas N., Embury S. H., Lubin B. H. A simple laboratory alternative to irreversibly sickled cell (ISC) counts. Blood. 1982 Sep;60(3):659–662. [PubMed] [Google Scholar]
  5. Clark M. R., Mohandas N., Shohet S. B. Deformability of oxygenated irreversibly sickled cells. J Clin Invest. 1980 Jan;65(1):189–196. doi: 10.1172/JCI109650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark M. R., Unger R. C., Shohet S. B. Monovalent cation composition and ATP and lipid content of irreversibly sickled cells. Blood. 1978 Jun;51(6):1169–1178. [PubMed] [Google Scholar]
  7. Corash L. M., Piomelli S., Chen H. C., Seaman C., Gross E. Separation of erythrocytes according to age on a simplified density gradient. J Lab Clin Med. 1974 Jul;84(1):147–151. [PubMed] [Google Scholar]
  8. Eaton J. W., Jacob H. S., White J. G. Membrane abnormalities of irreversibly sickled cells. Semin Hematol. 1979 Jan;16(1):52–64. [PubMed] [Google Scholar]
  9. Eisinger J., Flores J., Salhany J. M. Association of cytosol hemoglobin with the membrane in intact erythrocytes. Proc Natl Acad Sci U S A. 1982 Jan;79(2):408–412. doi: 10.1073/pnas.79.2.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Evans E. A. Bending elastic modulus of red blood cell membrane derived from buckling instability in micropipet aspiration tests. Biophys J. 1983 Jul;43(1):27–30. doi: 10.1016/S0006-3495(83)84319-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Evans E. A., Hochmuth R. M. A solid-liquid composite model of the red cell membrane. J Membr Biol. 1977 Jan 28;30(4):351–362. doi: 10.1007/BF01869676. [DOI] [PubMed] [Google Scholar]
  12. Evans E. A., La Celle P. L. Intrinsic material properties of the erythrocyte membrane indicated by mechanical analysis of deformation. Blood. 1975 Jan;45(1):29–43. [PubMed] [Google Scholar]
  13. Fabry M. E., Nagel R. L. Heterogeneity of red cells in the sickler: a characteristic with practical clinical and pathophysiological implications. Blood Cells. 1982;8(1):9–15. [PubMed] [Google Scholar]
  14. Havell T. C., Hillman D., Lessin L. S. Deformability characteristics of sickle cells by microelastimetry. Am J Hematol. 1978;4(1):9–16. doi: 10.1002/ajh.2830040103. [DOI] [PubMed] [Google Scholar]
  15. Hochmuth R. M., Worthy P. R., Evans E. A. Red cell extensional recovery and the determination of membrane viscosity. Biophys J. 1979 Apr;26(1):101–114. doi: 10.1016/S0006-3495(79)85238-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Klug P. P., Lessin L. S., Radice P. Rheological aspects of sickle cell disease. Arch Intern Med. 1974 Apr;133(4):577–590. [PubMed] [Google Scholar]
  17. Linderkamp O., Meiselman H. J. Geometric, osmotic, and membrane mechanical properties of density-separated human red cells. Blood. 1982 Jun;59(6):1121–1127. [PubMed] [Google Scholar]
  18. Messer M. J., Harris J. W. Filtration characteristics of sickle cells: rates of alteration of filterability after deoxygenation and reoxygenation, and correlations with sickling and unsickling. J Lab Clin Med. 1970 Oct;76(4):537–547. [PubMed] [Google Scholar]
  19. Murphy J. R., Wengard M., Brereton W. Rheological studies of Hb SS blood: influence of hematocrit, hypertonicity, separation of cells, deoxygenation, and mixture with normal cells. J Lab Clin Med. 1976 Mar;87(3):475–486. [PubMed] [Google Scholar]
  20. Noguchi C. T., Schechter A. N. The intracellular polymerization of sickle hemoglobin and its relevance to sickle cell disease. Blood. 1981 Dec;58(6):1057–1068. [PubMed] [Google Scholar]
  21. Noguchi C. T., Torchia D. A., Schechter A. N. Intracellular polymerization of sickle hemoglobin. Effects of cell heterogeneity. J Clin Invest. 1983 Sep;72(3):846–852. doi: 10.1172/JCI111055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Palek J., Thomae M., Ozog D. Red cell calcium content and transmembrane calcium movements in sickle cell anemia. J Lab Clin Med. 1977 Jun;89(6):1365–1374. [PubMed] [Google Scholar]
  23. Usami S., Chien S., Scholtz P. M., Bertles J. F. Effect of deoxygenation on blood rheology in sickle cell disease. Microvasc Res. 1975 May;9(3):324–334. doi: 10.1016/0026-2862(75)90069-2. [DOI] [PubMed] [Google Scholar]

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