Table 2.

Details of primers and citations used for the detection of capsular type and virulence associated genes in strains of Pasteurella multocida.

Gene	Primer	Primer sequence (5′-3′)	Reference
PM-PCR and Capsular serotypes
KMT1	PMPCR-F PMPCR-R	ATCCGCTATTTACCCAGTGG GCTGTAAACGAACTCGCCAC	[22]
hyaD-hyaC	capA F capA R	GATGCCAAAATCGCAGTCAG TGTTGCCATCATTGTCAGTG	[23]
bcbD	capB F capB R	CATTTATCCAAGCTCCACC GCCCGAGAGTTTCAATCC	[23]
dcbF	capD F capD R	TTACAAAAGAAAGACTAGGAGCCC CATCTACCCACTCAACCATATCAG	[23]
ecbJ	capE F capE R	TCCGCAGAAAATTATTGACTC GCTTGCTGCTTGATTTTGTC	[23]
fcbD	capF F capF R	AATCGGAGAACGCAGAAATCAG TTCCGCCGTCAATTACTCTG	[23]
Iron acquisition genes
tbpA	tbpA F tbpA R	GGACAGTGCATATAACTTGTT GGACAGTGCATATAACTTGTTTACTA	[32]
hgbA	hgbA F hgbA R	CATATCGGATCCTTGAAACCAGAGGAAGCAAAAA GAATCGGAGCTCACGACCTGGTGAGTAAAAACGAT	In-house [33]
hgbB	HgbB F HgbB R	ACCGCGTTGGAATTATGATTG CATTGAGTACGGCTTGACAT	[12]
Adhesins
ptfA	ptfA F ptfA R	AGGATCCATGAAAAAAGCCATTT GGAGCTCTTATGCGCAAAATCCTG	In-house
pfhA	pfhA F pfhA R	TAAGCCTATCGGTTCAAGTCG GATAAATCTACCCCGTCCTCT	In-house
Sialidases
NanB	NanB F NanB R	GTCCTATAAAGTGACGCCGA ACAGCAAAGGAAGACTGTCC	[12]
nanH	nanH F nanH R	CACTGCCTTATAGCCGTATTC AGCACTGTTACCCGAACCC	[12]
Dermonecrotoxin
ToxA	ToxA F ToxA R	TCTTAGATGAGCGACAAGG GAATGCCACACCTCTATAG	[34]