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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Virology. 2014 Sep 16;0:311–321. doi: 10.1016/j.virol.2014.08.022

Figure 3. BPV1 E2 co-immunoprecipitates with TIP60.

Figure 3

C33A (A) or HeLa (B) cells were transfected with expression plasmids for HA-tagged TIP60, FLAG-tagged F4 fragment of EP400 fragment, dual HA- and FLAG-tagged BPV1 E2 or the corresponding parental plasmids. 48 h post-transfection, cells were harvested and proteins immunoprecipitated with an antibody against FLAG. Total cellular proteins (Input) and bound proteins (IP:FLAG) were separated by SDS-PAGE and visualized by immunoblot analysis using antibodies against HA (detection of BPV1 E2 and TIP60), FLAG (F4 fragment of EP400 and BPV1 E2) and actin. Anti-Brd4 antibodies were used to detect Brd4 as a positive control for an E2 interacting protein in HeLa cells.