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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Virology. 2014 Aug 16;0:47–56. doi: 10.1016/j.virol.2014.07.038

Figure 5. Removal of the VHR does not alter the ability of T antigens to disrupt the pRB pathway.

Figure 5

A) Steady state levels of p130 decrease in MEFs upon expression of either ER or ERΔVHR constructs, as monitored by Western blot. GAPDH was used as a loading control. B) E2F-target gene expression is increased in all T antigen-expressing MEFs. The expression of four E2F-regulated genes was assessed by RT-PCR of RNA from 2 days post-confluent cells. Pcna: proliferating cell nuclear antigen; Dhfr: dihydrofolate reductase; Mcm3: minichromosome maintenance complex component 3; Cdc6: cell division cycle 6. Rpl5 is an endogenous control.