Figure 7. VHR truncated large T antigens retain the ability to bind p53.
Protein extracts were immunoprecipitated with a p53-specific antibody (PAb 421, SV40.ER and SV40.ERΔVHR samples) or a T antigen specific antibody (PAb 416, JCV.ER and JCV.ERΔVHR samples). Co-precipitated proteins were resolved by SDS-PAGE. The presence of large T and p53 was monitored by western blot.