Table 2.
Primers for Cloning | ||
SV40.ER | F | ACCATGGATAAAGTTTTAAACAGAGA |
R | TTATGTTTCAGGTTCAGGGG | |
SV40.ERΔVHR | F | ACCATGGATAAAGTTTTAAACAGAGAGG |
R | CTAAACTCCAATTCCCATAGC | |
JCV.ER | F | ACCATGGACAAAGTGCTGAATAGGG |
R | TTATTTTGGGGGAGGGGTCT | |
JCV.ERΔVHR | F | ACCATGGACAAAGTGCTGAATAGGG |
R | CTAAATGGGTCTCCCCATACCA | |
Primers for RT-PCR | ||
T Antigen | A | ACCATGGATAAAGTTTTAAACAGAGAGG |
B | ACCGAGGAGAGGGTTAGGGAT | |
C | CTGACTTTGGAGGCTTCTGG | |
D | AAGGAAAGTCCTTGGGGTCT | |
E | ACCATGGACAAAGTGCTGAATAGGG | |
F | GCCTGATTTTGGTACATGGAA | |
G | ACAGCTTGACTGAGGAATGC | |
Pcna | F | AGGCTCTCAAAGACCTCATCAATG |
R | CCTGTTCTGGGATTCCAAGTTG | |
Dhfr | F | GTAGAGAACTCAAAGAACCACCACG |
R | TTTTCCTCCTGGACCTCAGAGAG | |
Mcm | F | CGCAGGAAGAATGAAAAGAGGG |
R | CTGAGGAAGCAGGAAGTGAGAGTC | |
Cdc6 | F | AGTTCTGTGCCCGCAAAGTG |
R | AGCAGCAAAGAGCAAACCAGG | |
Rpl5 | F | CCAAACGATTCCCTGGTTATGAC |
R | GACGATTCCACCTCTTCTTCTTCAC |