Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1984 Dec;74(6):2089–2096. doi: 10.1172/JCI111633

Decreased expression of human class II antigens on monocytes from patients with acquired immune deficiency syndrome. Increased expression with interferon-gamma.

W Heagy, V E Kelley, T B Strom, K Mayer, H M Shapiro, R Mandel, R Finberg
PMCID: PMC425399  PMID: 6439741

Abstract

The expression of HLA-DR (a class II histocompatibility antigen) on monocytes isolated from the peripheral blood of normal individuals and patients with acquired immune deficiency syndrome (AIDS) was investigated by the use of dual fluorescent staining and cytofluorometry. In animal models the absence of class II positive monocytes is linked to a failure of T cells to respond to antigens. We now report that patients with AIDS have a paucity of HLA-DR+ monocytes. The percentage of HLA-DR+ monocytes among eight normal individuals ranged from 49.3 to 95.0%+, and only one individual had less than 50% HLA-DR+ monocytes. HLA-DR expression on monocytes from homosexual male patients with lymphadenopathy was similar to that of normal subjects (range, 58.0 to 97.4%+). In contrast, seven of nine patients with AIDS had less than 50% HLA-DR+ monocytes (range, 13.4 to 78.8%+). The in vitro incubation of monocytes from AIDS patients with cloned human interferon-gamma resulted in an increase of the expression of HLA-DR to near normal levels.

Full text

PDF
2089

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ameglio F., Capobianchi M. R., Dolei A., Tosi R. Differential effects of gamma interferon on expression of HLA class II molecules controlled by the DR and DC loci. Infect Immun. 1983 Oct;42(1):122–125. doi: 10.1128/iai.42.1.122-125.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barré-Sinoussi F., Chermann J. C., Rey F., Nugeyre M. T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vézinet-Brun F., Rouzioux C. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):868–871. doi: 10.1126/science.6189183. [DOI] [PubMed] [Google Scholar]
  3. Basham T. Y., Merigan T. C. Recombinant interferon-gamma increases HLA-DR synthesis and expression. J Immunol. 1983 Apr;130(4):1492–1494. [PubMed] [Google Scholar]
  4. Belsito D. V., Sanchez M. R., Baer R. L., Valentine F., Thorbecke G. J. Reduced Langerhans' cell Ia antigen and ATPase activity in patients with the acquired immunodeficiency syndrome. N Engl J Med. 1984 May 17;310(20):1279–1282. doi: 10.1056/NEJM198405173102002. [DOI] [PubMed] [Google Scholar]
  5. Bodmer W. F. HLA structure and function: a contemporary view. Tissue Antigens. 1981 Jan;17(1):9–20. doi: 10.1111/j.1399-0039.1981.tb00661.x. [DOI] [PubMed] [Google Scholar]
  6. Bono M. R., Strominger J. L. Direct evidence of homology between human DC-1 antigen and murine I-A molecules. Nature. 1982 Oct 28;299(5886):836–840. doi: 10.1038/299836a0. [DOI] [PubMed] [Google Scholar]
  7. Devos R., Cheroutre H., Taya Y., Degrave W., Van Heuverswyn H., Fiers W. Molecular cloning of human immune interferon cDNA and its expression in eukaryotic cells. Nucleic Acids Res. 1982 Apr 24;10(8):2487–2501. doi: 10.1093/nar/10.8.2487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Essex M., McLane M. F., Lee T. H., Falk L., Howe C. W., Mullins J. I., Cabradilla C., Francis D. P. Antibodies to cell membrane antigens associated with human T-cell leukemia virus in patients with AIDS. Science. 1983 May 20;220(4599):859–862. doi: 10.1126/science.6342136. [DOI] [PubMed] [Google Scholar]
  9. Finberg R., Benacerraf B. Induction, control and consequences of virus specific cytotoxic T cells. Immunol Rev. 1981;58:157–180. doi: 10.1111/j.1600-065x.1981.tb00353.x. [DOI] [PubMed] [Google Scholar]
  10. Gallo R. C., Sarin P. S., Gelmann E. P., Robert-Guroff M., Richardson E., Kalyanaraman V. S., Mann D., Sidhu G. D., Stahl R. E., Zolla-Pazner S. Isolation of human T-cell leukemia virus in acquired immune deficiency syndrome (AIDS). Science. 1983 May 20;220(4599):865–867. doi: 10.1126/science.6601823. [DOI] [PubMed] [Google Scholar]
  11. Gelmann E. P., Popovic M., Blayney D., Masur H., Sidhu G., Stahl R. E., Gallo R. C. Proviral DNA of a retrovirus, human T-cell leukemia virus, in two patients with AIDS. Science. 1983 May 20;220(4599):862–865. doi: 10.1126/science.6601822. [DOI] [PubMed] [Google Scholar]
  12. Gonwa T. A., Picker L. J., Raff H. V., Goyert S. M., Silver J., Stobo J. D. Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR. J Immunol. 1983 Feb;130(2):706–711. [PubMed] [Google Scholar]
  13. Gottlieb M. S., Schroff R., Schanker H. M., Weisman J. D., Fan P. T., Wolf R. A., Saxon A. Pneumocystis carinii pneumonia and mucosal candidiasis in previously healthy homosexual men: evidence of a new acquired cellular immunodeficiency. N Engl J Med. 1981 Dec 10;305(24):1425–1431. doi: 10.1056/NEJM198112103052401. [DOI] [PubMed] [Google Scholar]
  14. Groopman J. E., Gottlieb M. S. Kaposi's sarcoma: an oncologic looking glass. Nature. 1982 Sep 9;299(5879):103–104. doi: 10.1038/299103a0. [DOI] [PubMed] [Google Scholar]
  15. Hardy R. R., Hayakawa K., Haaijman J., Herzenberg L. A. B-cell subpopulations identified by two-colour fluorescence analysis. Nature. 1982 Jun 17;297(5867):589–591. doi: 10.1038/297589a0. [DOI] [PubMed] [Google Scholar]
  16. Haverkos H. W., Curran J. W. The current outbreak of Kaposi's sarcoma and opportunistic infections. CA Cancer J Clin. 1982 Nov-Dec;32(6):330–339. doi: 10.3322/canjclin.32.6.330. [DOI] [PubMed] [Google Scholar]
  17. Hurley C. K., Shaw S., Nadler L., Schlossman S., Capra J. D. Alpha and beta chains of SB and DR antigens are structurally distinct. J Exp Med. 1982 Nov 1;156(5):1557–1562. doi: 10.1084/jem.156.5.1557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hämmerling G. J. Tissue distribution of Ia antigens and their expression on lymphocyte subpopulations. Transplant Rev. 1976;30:64–82. [PubMed] [Google Scholar]
  19. Kasahara T., Hooks J. J., Dougherty S. F., Oppenheim J. J. Interleukin 2-mediated immune interferon (IFN-gamma) production by human T cells and T cell subsets. J Immunol. 1983 Apr;130(4):1784–1789. [PubMed] [Google Scholar]
  20. Kawase I., Brooks C. G., Kuribayashi K., Olabuenaga S., Newman W., Gillis S., Henney C. S. Interleukin 2 induces gamma-interferon production: participation of macrophages and NK-like cells. J Immunol. 1983 Jul;131(1):288–292. [PubMed] [Google Scholar]
  21. Kelley V. E., Fiers W., Strom T. B. Cloned human interferon-gamma, but not interferon-beta or -alpha, induces expression of HLA-DR determinants by fetal monocytes and myeloid leukemic cell lines. J Immunol. 1984 Jan;132(1):240–245. [PubMed] [Google Scholar]
  22. Kornfeld H., Vande Stouwe R. A., Lange M., Reddy M. M., Grieco M. H. T-lymphocyte subpopulations in homosexual men. N Engl J Med. 1982 Sep 16;307(12):729–731. doi: 10.1056/NEJM198209163071206. [DOI] [PubMed] [Google Scholar]
  23. Lampert I. A., Suitters A. J., Chisholm P. M. Expression of Ia antigen on epidermal keratinocytes in graft-versus-host disease. Nature. 1981 Sep 10;293(5828):149–150. doi: 10.1038/293149a0. [DOI] [PubMed] [Google Scholar]
  24. Letvin N. L., Kauffman R. S., Finberg R. T lymphocyte immunity to reovirus: cellular requirements for generation and role in clearance of primary infections. J Immunol. 1981 Dec;127(6):2334–2339. [PubMed] [Google Scholar]
  25. Mason D. W., Dallman M., Barclay A. N. Graft-versus-host disease induces expression of Ia antigen in rat epidermal cells and gut epithelium. Nature. 1981 Sep 10;293(5828):150–151. doi: 10.1038/293150a0. [DOI] [PubMed] [Google Scholar]
  26. Masur H., Michelis M. A., Greene J. B., Onorato I., Stouwe R. A., Holzman R. S., Wormser G., Brettman L., Lange M., Murray H. W. An outbreak of community-acquired Pneumocystis carinii pneumonia: initial manifestation of cellular immune dysfunction. N Engl J Med. 1981 Dec 10;305(24):1431–1438. doi: 10.1056/NEJM198112103052402. [DOI] [PubMed] [Google Scholar]
  27. Mildvan D., Mathur U., Enlow R. W., Romain P. L., Winchester R. J., Colp C., Singman H., Adelsberg B. R., Spigland I. Opportunistic infections and immune deficiency in homosexual men. Ann Intern Med. 1982 Jun;96(6 Pt 1):700–704. doi: 10.7326/0003-4819-96-6-700. [DOI] [PubMed] [Google Scholar]
  28. Murray H. W., Rubin B. Y., Masur H., Roberts R. B. Impaired production of lymphokines and immune (gamma) interferon in the acquired immunodeficiency syndrome. N Engl J Med. 1984 Apr 5;310(14):883–889. doi: 10.1056/NEJM198404053101404. [DOI] [PubMed] [Google Scholar]
  29. Nunez G., Stastny P. Cytofluorometric analysis of major histocompatibility antigens on human monocytes using monoclonal antibodies. Hum Immunol. 1983 Jan;6(1):1–11. doi: 10.1016/0198-8859(83)90069-1. [DOI] [PubMed] [Google Scholar]
  30. O'Malley J. A., Nussbaum-Blumenson A., Sheedy D., Grossmayer B. J., Ozer H. Identification of the T cell subset that produces human gamma interferon. J Immunol. 1982 Jun;128(6):2522–2526. [PubMed] [Google Scholar]
  31. Pober J. S., Gimbrone M. A., Jr, Cotran R. S., Reiss C. S., Burakoff S. J., Fiers W., Ault K. A. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon. J Exp Med. 1983 Apr 1;157(4):1339–1353. doi: 10.1084/jem.157.4.1339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Rook A. H., Masur H., Lane H. C., Frederick W., Kasahara T., Macher A. M., Djeu J. Y., Manischewitz J. F., Jackson L., Fauci A. S. Interleukin-2 enhances the depressed natural killer and cytomegalovirus-specific cytotoxic activities of lymphocytes from patients with the acquired immune deficiency syndrome. J Clin Invest. 1983 Jul;72(1):398–403. doi: 10.1172/JCI110981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Scahill S. J., Devos R., Van der Heyden J., Fiers W. Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4654–4658. doi: 10.1073/pnas.80.15.4654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Shackelford D. A., Mann D. L., van Rood J. J., Ferrara G. B., Strominger J. L. Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigen. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4566–4570. doi: 10.1073/pnas.78.7.4566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Shaw S., DeMars R., Schlossman S. F., Smith P. L., Lampson L. A., Nadler L. M. Serologic identification of the human secondary B cell antigens. Correlations between function, genetics, and structure. J Exp Med. 1982 Sep 1;156(3):731–743. doi: 10.1084/jem.156.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Siegal F. P., Lopez C., Hammer G. S., Brown A. E., Kornfeld S. J., Gold J., Hassett J., Hirschman S. Z., Cunningham-Rundles C., Adelsberg B. R. Severe acquired immunodeficiency in male homosexuals, manifested by chronic perianal ulcerative herpes simplex lesions. N Engl J Med. 1981 Dec 10;305(24):1439–1444. doi: 10.1056/NEJM198112103052403. [DOI] [PubMed] [Google Scholar]
  37. Sztein M. B., Steeg P. S., Johnson H. M., Oppenheim J. J. Regulation of human peripheral blood monocyte DR antigen expression in vitro by lymphokines and recombinant interferons. J Clin Invest. 1984 Feb;73(2):556–565. doi: 10.1172/JCI111243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Termijtelen A., van Leeuwen A., van Rood J. J. HLA-linked lymphocyte activating determinants. Immunol Rev. 1982;66:79–101. doi: 10.1111/j.1600-065x.1982.tb00435.x. [DOI] [PubMed] [Google Scholar]
  39. Todd R. F., 3rd, Nadler L. M., Schlossman S. F. Antigens on human monocytes identified by monoclonal antibodies. J Immunol. 1981 Apr;126(4):1435–1442. [PubMed] [Google Scholar]
  40. Torres B. A., Farrar W. L., Johnson H. M. Interleukin 2 regulates immune interferon (IFN gamma) production by normal and suppressor cell cultures. J Immunol. 1982 May;128(5):2217–2219. [PubMed] [Google Scholar]
  41. Urmacher C., Myskowski P., Ochoa M., Jr, Kris M., Safai B. Outbreak of Kaposi's sarcoma with cytomegalovirus infection in young homosexual men. Am J Med. 1982 Apr;72(4):569–575. doi: 10.1016/0002-9343(82)90450-8. [DOI] [PubMed] [Google Scholar]
  42. Van Voorhis W. C., Steinman R. M., Hair L. S., Luban J., Witmer M. D., Koide S., Cohn Z. A. Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages. J Exp Med. 1983 Jul 1;158(1):126–145. doi: 10.1084/jem.158.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Yamashita U., Shevach E. M. The expression of Ia antigens on immunocompetent cells in the guinea pig. II. Ia antigens on macrophages. J Immunol. 1977 Nov;119(5):1584–1588. [PubMed] [Google Scholar]
  44. Yilma T., Perryman L. E., McGuire T. C. Deficiency of interferon-gamma but not interferon-beta in Arabian foals with severe combined immunodeficiency. J Immunol. 1982 Sep;129(3):931–933. [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES