Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1984 Dec;74(6):2279–2284. doi: 10.1172/JCI111655

Effect of monocyte migration on low density lipoprotein transport across aortic endothelial cell monolayers.

M Territo, J A Berliner, A M Fogelman
PMCID: PMC425421  PMID: 6511926

Abstract

Endothelial cell monolayers on polycarbonate filters present a barrier to low density lipoprotein (LDL) and albumin transport. These cells form a relatively tight monolayer as shown by measurements of electrical resistance across the monolayer (15 omega-cm2). Monocytes are able to migrate freely across the monolayers in response to chemotactic stimuli. Monocyte chemotaxis across the monolayer caused a marked increase in LDL and albumin transport across the monolayer in the direction of monocyte migration. However, transport in the opposite direction was not significantly increased. These results suggest that monocyte migration across the endothelium could lead to an increased LDL content of the intima.

Full text

PDF
2279

Images in this article

2280Image
on p.2280
2280Image
on p.2280
2282Image
on p.2282

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell F. P., Gallus A. S., Schwartz C. J. Focal and regional patterns of uptake and the transmural distribution of 131-I-fibrinogen in the pig aorta in vivo. Exp Mol Pathol. 1974 Apr;20(2):281–292. doi: 10.1016/0014-4800(74)90060-4. [DOI] [PubMed] [Google Scholar]
  2. Berliner J. A. Formation of enlarged mitochondria in a liver cell line in response to a synthetic glucocorticoid. J Cell Biol. 1975 Mar;64(3):711–716. doi: 10.1083/jcb.64.3.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bratzler R. L., Chisolm G. M., Colton C. K., Smith K. A., Lees R. S. The distribution of labeled low-density lipoproteins across the rabbit thoracic aorta in vivo. Atherosclerosis. 1977 Nov;28(3):289–307. doi: 10.1016/0021-9150(77)90177-0. [DOI] [PubMed] [Google Scholar]
  4. Bussolari S. R., Dewey C. F., Jr, Gimbrone M. A., Jr Apparatus for subjecting living cells to fluid shear stress. Rev Sci Instrum. 1982 Dec;53(12):1851–1854. doi: 10.1063/1.1136909. [DOI] [PubMed] [Google Scholar]
  5. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  6. Chamley-Campbell J., Campbell G. R., Ross R. The smooth muscle cell in culture. Physiol Rev. 1979 Jan;59(1):1–61. doi: 10.1152/physrev.1979.59.1.1. [DOI] [PubMed] [Google Scholar]
  7. Chobanian A. V., Menzoian J. O., Shipman J., Heath K., Haudenschild C. C. Effects of endothelial denudation and cholesterol feeding on in vivo transport of albumin, glucose, and water across rabbit carotid artery. Circ Res. 1983 Dec;53(6):805–814. doi: 10.1161/01.res.53.6.805. [DOI] [PubMed] [Google Scholar]
  8. Davies P. F., Kerr C. Co-cultivation of vascular endothelial and smooth muscle cells using microcarrier techniques. Exp Cell Res. 1982 Oct;141(2):455–459. doi: 10.1016/0014-4827(82)90234-8. [DOI] [PubMed] [Google Scholar]
  9. FLOREY H. W., GRANT L. H. Leucocyte migration from small blood vessels stimulated with ultraviolet light: an electron-microscope study. J Pathol Bacteriol. 1961 Jul;82:13–17. doi: 10.1002/path.1700820103. [DOI] [PubMed] [Google Scholar]
  10. Fogelman A. M., Haberland M. E., Seager J., Hokom M., Edwards P. A. Factors regulating the activities of the low density lipoprotein receptor and the scavenger receptor on human monocyte-macrophages. J Lipid Res. 1981 Sep;22(7):1131–1141. [PubMed] [Google Scholar]
  11. Fogelman A. M., Shechter I., Seager J., Hokom M., Child J. S., Edwards P. A. Malondialdehyde alteration of low density lipoproteins leads to cholesteryl ester accumulation in human monocyte-macrophages. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2214–2218. doi: 10.1073/pnas.77.4.2214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gerrity R. G. The role of the monocyte in atherogenesis: I. Transition of blood-borne monocytes into foam cells in fatty lesions. Am J Pathol. 1981 May;103(2):181–190. [PMC free article] [PubMed] [Google Scholar]
  13. Hüttner I., Boutet M., More R. H. Studies on protein passage through arterial endothelium. I. Structural correlates of permeability in rat arterial endothelium. Lab Invest. 1973 Jun;28(6):672–677. [PubMed] [Google Scholar]
  14. Joris I., Stetz E., Majno G. Lymphocytes and monocytes in the aortic intima--An electron-microscopic study in the rat. Atherosclerosis. 1979 Nov;34(3):221–231. doi: 10.1016/s0021-9150(79)80003-9. [DOI] [PubMed] [Google Scholar]
  15. Keller H. U., Hess M. W., Cottier H. The in vitro assessment of leucocyte chemotaxis. Antibiot Chemother (1971) 1974;19:112–125. doi: 10.1159/000395427. [DOI] [PubMed] [Google Scholar]
  16. Kim K. J., Crandall E. D. Effects of exposure to acid on alveolar epithelial water and solute transport. J Appl Physiol Respir Environ Exerc Physiol. 1982 Apr;52(4):902–909. doi: 10.1152/jappl.1982.52.4.902. [DOI] [PubMed] [Google Scholar]
  17. MARCHESI V. T. The site of leucocyte emigration during inflammation. Q J Exp Physiol Cogn Med Sci. 1961 Apr;46:115–118. doi: 10.1113/expphysiol.1961.sp001522. [DOI] [PubMed] [Google Scholar]
  18. Ross R. George Lyman Duff Memorial Lecture. Atherosclerosis: a problem of the biology of arterial wall cells and their interactions with blood components. Arteriosclerosis. 1981 Sep-Oct;1(5):293–311. doi: 10.1161/01.atv.1.5.293. [DOI] [PubMed] [Google Scholar]
  19. Schwartz S. M. Selection and characterization of bovine aortic endothelial cells. In Vitro. 1978 Dec;14(12):966–980. doi: 10.1007/BF02616210. [DOI] [PubMed] [Google Scholar]
  20. Smith E. B., Staples E. M. Distribution of plasma proteins across the human aortic wall--barrier functions of endothelium and internal elastic lamina. Atherosclerosis. 1980 Dec;37(4):579–590. doi: 10.1016/0021-9150(80)90065-9. [DOI] [PubMed] [Google Scholar]
  21. Taylor R. F., Price T. H., Schwartz S. M., Dale D. C. Neutrophil-endothelial cell interactions on endothelial monolayers grown on micropore filters. J Clin Invest. 1981 Feb;67(2):584–587. doi: 10.1172/JCI110071. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES