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. 1985 May;75(5):1435–1440. doi: 10.1172/JCI111845

Rabbits immunized with thyroid-stimulating hormone produce autoantiidiotypic thyroid-stimulating antibodies.

G N Beall, B Rapoport, I J Chopra, S R Kruger
PMCID: PMC425480  PMID: 2860123

Abstract

We immunized rabbits with thyroid-stimulating hormone (TSH) to investigate the hypothesis that such immunization could result in production of thyroid-stimulating autoantiidiotypic antibodies to anti-TSH. Thyroid-stimulating immunoglobulin (TSI) appeared in the serum of several rabbits after immunization. At 160 d, TSI equivalent to 6-18 microU TSH/1.5 mg IgG was present in two of six human (h)TSH-, two of six hTSH beta chain-, and two of the four surviving bovine (b)TSH-immunized animals. Control (human serum albumin-immunized rabbits) serum TSI was 4.3 +/- 0.4 (mean +/- SD) at this time. Antiidiotypic antibodies that could bind to monoclonal anti-hTSH were found in the sera of the bTSH-immunized rabbits. The peak TSI activity occurred 3 mo after a TSH booster immunization and declined gradually during subsequent weeks. Evidence that antiidiotypic antibodies to anti-TSH can cause thyroid stimulation strengthens the notion that such antibodies may be the cause of Graves' hyperthyroidism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beall G. N., Chopra I. J., Solomon D. H., Irvine W. J., Kruger S. R. Studies of the TSH radioreceptor assay. Acta Endocrinol (Copenh) 1979 Feb;90(2):217–226. doi: 10.1530/acta.0.0900217. [DOI] [PubMed] [Google Scholar]
  2. Beall G. N., Chopra I. J., Solomon D. H., Pierce J. G., Cornell J. S. Neutralizing and non-neutralizing antibodies to bovine thyroid-stimulating hormone and its subunits. J Clin Invest. 1973 Dec;52(12):2979–2985. doi: 10.1172/JCI107495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beall G. N., Chopra I. J., Solomon D. H., Pierce J. G., Cornell J. S. The effects on rabbits of immunization with bovine thyroid-stimulating hormone and its subunits. J Clin Invest. 1973 Dec;52(12):2986–2992. doi: 10.1172/JCI107496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beall G. N., Kruger S. R. Binding of 125I-human TSH by gamma globulins of sera containing thyroid-stimulating immunoglobulin (TSI). Life Sci. 1983 Jan 3;32(1-2):77–83. doi: 10.1016/0024-3205(83)90175-3. [DOI] [PubMed] [Google Scholar]
  5. Bíró J. Specific binding of thyroid-stimulating hormone by human serum globulins. J Endocrinol. 1981 Mar;88(3):339–349. doi: 10.1677/joe.0.0880339. [DOI] [PubMed] [Google Scholar]
  6. Bíró J. TSH binding by globulins from patients with Graves' disease. Endocrinol Exp. 1981;15(2):145–153. [PubMed] [Google Scholar]
  7. Chopra I. J. A radioimmunoassay for measurement of thyroxine in unextracted serum. J Clin Endocrinol Metab. 1972 Jun;34(6):938–947. doi: 10.1210/jcem-34-6-938. [DOI] [PubMed] [Google Scholar]
  8. Chopra I. J., Solomon D. H. Pathogenesis of hyperthyroidism. Annu Rev Med. 1983;34:267–281. doi: 10.1146/annurev.me.34.020183.001411. [DOI] [PubMed] [Google Scholar]
  9. Geha R. S. Presence of circulating anti-idiotype-bearing cells after booster immunization with tetanus toxoid (TT) and inhibition of anti-TT antibody synthesis by auto-anti-idiotypic antibody. J Immunol. 1983 Apr;130(4):1634–1639. [PubMed] [Google Scholar]
  10. Islam M. N., Pepper B. M., Briones-Urbina R., Farid N. R. Biological activity of anti-thyrotropin anti-idiotypic antibody. Eur J Immunol. 1983 Jan;13(1):57–63. doi: 10.1002/eji.1830130113. [DOI] [PubMed] [Google Scholar]
  11. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  12. Marasco W. A., Becker E. L. Anti-idiotype as antibody against the formyl peptide chemotaxis receptor of the neutrophil. J Immunol. 1982 Feb;128(2):963–968. [PubMed] [Google Scholar]
  13. Pekary A. E., Hershman J. M., Parlow A. F. A sensitive and precise radioimmunoassay for human thyroid-stimulating hormone. J Clin Endocrinol Metab. 1975 Oct;41(4):676–684. doi: 10.1210/jcem-41-4-676. [DOI] [PubMed] [Google Scholar]
  14. Pinchera A., Fenzi G. F., Macchia E., Bartalena L., Mariotti S., Monzani F. Thyroid-stimulating immunoglobulins. Horm Res. 1982;16(5):317–328. doi: 10.1159/000179520. [DOI] [PubMed] [Google Scholar]
  15. Rapoport B., Filetti S., Takai N., Seto P., Halverson G. Studies on the cyclic AMP response to thyroid stimulating immunoglobulin (TSI) and thyrotropin (TSH) in human thyroid cell monolayers. Metabolism. 1982 Nov;31(11):1159–1167. doi: 10.1016/0026-0495(82)90168-8. [DOI] [PubMed] [Google Scholar]
  16. Rapoport B., Greenspan F. S., Filetti S., Pepitone M. Clinical experience with a human thyroid cell bioassay for thyroid-stimulating immunoglobulin. J Clin Endocrinol Metab. 1984 Feb;58(2):332–338. doi: 10.1210/jcem-58-2-332. [DOI] [PubMed] [Google Scholar]
  17. Schreiber A. B., Couraud P. O., Andre C., Vray B., Strosberg A. D. Anti-alprenolol anti-idiotypic antibodies bind to beta-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7385–7389. doi: 10.1073/pnas.77.12.7385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sege K., Peterson P. A. Use of anti-idiotypic antibodies as cell-surface receptor probes. Proc Natl Acad Sci U S A. 1978 May;75(5):2443–2447. doi: 10.1073/pnas.75.5.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shechter Y., Maron R., Elias D., Cohen I. R. Autoantibodies to insulin receptor spontaneously develop as anti-idiotypes in mice immunized with insulin. Science. 1982 Apr 30;216(4545):542–545. doi: 10.1126/science.7041258. [DOI] [PubMed] [Google Scholar]

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