Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1985 Jun;75(6):1977–1982. doi: 10.1172/JCI111915

A human T cell lymphoma secreting an immunoglobulin E specific helper factor.

M C Young, H Harfi, R Sabbah, D Y Leung, R S Geha
PMCID: PMC425557  PMID: 3159754

Abstract

An 8-yr-old nonallergic girl with non-Hodgkin's lymphoma had markedly elevated serum IgE at presentation (greater than 10,000 IU/ml), negative skin tests to a battery of 24 common allergens, and no evidence of parasitic infestation. Serum levels of IgG, IgA, and IgM were normal. Remission after cytotoxic chemotherapy was accompanied by a marked reduction in serum IgE levels (to less than 200 IU/ml) with no change in the level of serum IgG, IgM, or IgA. Recurrence of the lymphoma 7 mo after remission was accompanied by an isotype specific rise in serum IgE (to 3,850 IU/ml). Isoelectric focusing revealed that the IgE was polyclonal. Phenotypic analysis of the lymphoma obtained during relapse revealed all (greater than 98%) cells to be T3+, T4+, and T8+. Incubation of lymphoma cells with human myeloma IgE followed by immunosorbent purified fluorescein tagged goat anti-human IgE (anti-IgE PS-adsorbed over IgE ADZ) stained 25% of the cells. In contrast, less than 1% of the cells were stained after incubation with human IgG followed by fluorescein conjugated goat anti-human IgE. Supernatants from lymphoma cells (5 X 10(6)/ml, 48 h) enhanced IgE production in B cells derived from four patients with allergic rhinitis (mean +/- SD picograms per milliliter of net IgE 930 +/- 320 in unstimulated cultures versus 2,450 +/- 650 in cultures stimulated with lymphoma supernatants; P less than 0.01) but did not induce IgE synthesis in B cells from two normal subjects that synthesized no IgE spontaneously. Lymphoma supernatants failed to enhance IgG synthesis by B cells of both allergic and nonallergic subjects. These results indicate that a T cell lymphoma comprised of cells bearing Fc receptors for IgE with a phenotype characteristic of immature T cells (i.e., T3+, T4+, T8+) exhibited IgE specific helper function. This lymphoma may represent the monoclonal expansion of a subpopulation of IgE specific helper T cells.

Full text

PDF
1977

Images in this article

1978Image
on p.1978

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhan A. K., Reinherz E. L., Poppema S., McCluskey R. T., Schlossman S. F. Location of T cell and major histocompatibility complex antigens in the human thymus. J Exp Med. 1980 Oct 1;152(4):771–782. doi: 10.1084/jem.152.4.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Geha R. S., Reinherz E., Leung D., McKee K. T., Jr, Schlossman S., Rosen F. S. Deficiency of suppressor T cells in the hyperimmunoglobulin E syndrome. J Clin Invest. 1981 Sep;68(3):783–791. doi: 10.1172/JCI110315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Geha R. S., Rosen F. S., Merler E. Identification and characterization of subpopulations of lymphocytes in human peripheral blood after fractionation on discontinuous gradients of albumin. The cellular defect in X-linked agammaglobulinemia. J Clin Invest. 1973 Jul;52(7):1726–1734. doi: 10.1172/JCI107354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirashima M., Yodoi J., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. III. IgE-specific suppressive factor with IgE-binding activity. J Immunol. 1980 Oct;125(4):1442–1448. [PubMed] [Google Scholar]
  5. Inganäs M., Johansson S. G., Bennich H. Anti-IgE antibodies in human serum: occurrence and specificity. Int Arch Allergy Appl Immunol. 1981;65(1):51–61. doi: 10.1159/000232737. [DOI] [PubMed] [Google Scholar]
  6. Ishizaka K., Ishizaka T. Mechanisms of reaginic hypersensitivity and IgE antibody response. Immunol Rev. 1978;41:109–148. doi: 10.1111/j.1600-065x.1978.tb01462.x. [DOI] [PubMed] [Google Scholar]
  7. Ishizaka K. Regulation of IgE response by IgE-binding factors. Monogr Allergy. 1983;18:52–60. [PubMed] [Google Scholar]
  8. Katz D. H. Recent studies on the regulation of IgE antibody synthesis in experimental animals and man. Immunology. 1980 Sep;41(1):1–24. [PMC free article] [PubMed] [Google Scholar]
  9. Krensky A. M., Reiss C. S., Mier J. W., Strominger J. L., Burakoff S. J. Long-term human cytolytic T-cell lines allospecific for HLA-DR6 antigen are OKT4+. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2365–2369. doi: 10.1073/pnas.79.7.2365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leung D. Y., Rhodes A. R., Geha R. S. Enumeration of T cell subsets in atopic dermatitis using monoclonal antibodies. J Allergy Clin Immunol. 1981 Jun;67(6):450–455. doi: 10.1016/0091-6749(81)90098-1. [DOI] [PubMed] [Google Scholar]
  11. Meuer S. C., Hussey R. E., Hodgdon J. C., Hercend T., Schlossman S. F., Reinherz E. L. Surface structures involved in target recognition by human cytotoxic T lymphocytes. Science. 1982 Oct 29;218(4571):471–473. doi: 10.1126/science.6981845. [DOI] [PubMed] [Google Scholar]
  12. Mitsuya H., Sato M., Hirano T., Fujimoto K., Kawano F., Kishimoto S. Evidence for a malignant proliferation of IgE-class specific helper T cells in a patient with Sézary syndrome exhibiting massive hyperimmunoglobulinemia E. Clin Immunol Immunopathol. 1983 Feb;26(2):171–183. doi: 10.1016/0090-1229(83)90135-6. [DOI] [PubMed] [Google Scholar]
  13. Reinherz E. L., Cooper M. D., Schlossman S. F., Rosen F. S. Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders. J Clin Invest. 1981 Sep;68(3):699–705. doi: 10.1172/JCI110305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Saryan J. A., Leung D. Y., Geha R. S. Induction of human IgE synthesis by a factor derived from T cells of patients with hyper-IgE states. J Immunol. 1983 Jan;130(1):242–247. [PubMed] [Google Scholar]
  15. Turner K. J., Holt P. G., Holt B. J., Cameron K. J. In vitro synthesis of IgE by human peripheral blood leucocytes. III. Release of pre-formed antibody. Clin Exp Immunol. 1983 Feb;51(2):387–394. [PMC free article] [PubMed] [Google Scholar]
  16. Wood B. T., Thompson S. H., Goldstein G. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies. J Immunol. 1965 Aug;95(2):225–229. [PubMed] [Google Scholar]
  17. Yodoi J., Hirashima M., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. V. Effect of tunicamycin on the formation of IgE-potentiating factor and IgE-suppressive factor by con A-activated lymphocytes. J Immunol. 1981 Mar;126(3):877–882. [PubMed] [Google Scholar]
  18. Yodoi J., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. IV. Formation of IgE-binding factor by rat T lymphocytes. J Immunol. 1980 Mar;124(3):1322–1329. [PubMed] [Google Scholar]
  19. Young M. C., Leung D. Y., Geha R. S. Production of IgE-potentiating factor in man by T cell lines bearing Fc receptors for IgE. Eur J Immunol. 1984 Oct;14(10):871–878. doi: 10.1002/eji.1830141003. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES