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. 1981 Jan;67(1):47–53. doi: 10.1104/pp.67.1.47

Functional and Structural Organization of Chlorophyll in the Developing Photosynthetic Membranes of Euglena gracilis Z

II. CHARACTERISTICS OF THE LATE FORMATION OF ACTIVE PHOTOSYSTEM II REACTION CENTERS DURING FIRST STAGES OF GREENING 1

Guy Dubertret 1, Françoise Ambard-Bretteville 1
PMCID: PMC425619  PMID: 16661631

Abstract

During light-induced greening of dark-grown, nondividing Euglena gracilis Z, there is a delay of about 10 hours in the formation of active photosystem II (PSII) reaction centers compared to chlorophyll synthesis. Experiments with greening under different light intensities rule out the possibility that this delay results from a late induction of active PSII reaction center formation when a definite amount of chlorophyll is attained in the early greened cells. Experiments on greening after preillumination show that this delay does not originate in a long, light-induced formation of specific synthesizing machinery for reaction center components. Experiments with greening in the presence of streptomycin show that, when this inhibitor of protein synthesis by chloroplastic ribosomes is added to dark-grown, preilluminated cells or to cells already greened for 24 hours, the formation of active PSII reaction centers is inhibited after a time which depends on the light intensity used for greening. Under very low light intensity (150 lux), the addition of streptomycin to 24-hour greened cells does not prevent further development of functional chloroplasts. These observations lead to the conclusion that streptomycin-insensitive chloro-plast development occurs due to syntheses of cytoplasmic origin and of light-induced pools of components synthesized early by chloroplastic ribo-somes. Conformational changes requiring time may allow the insertion of components necessary for the reorganization of PSII reaction centers in the developing thylakoid after synthesis. This hypothesis accounts for the observed delay in PSII reaction center formation compared to chlorophyll synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akoyunoglou G. Development of the photosystem II unit in plastids of bean leaves greened in periodic light. Arch Biochem Biophys. 1977 Oct;183(2):571–580. doi: 10.1016/0003-9861(77)90392-7. [DOI] [PubMed] [Google Scholar]
  2. Avadhani N. G., Buetow D. E. Isolation of active polyribosomes from the cytoplasm, mitochondria and chloroplasts of Euglena gracilis. Biochem J. 1972 Jun;128(2):353–365. doi: 10.1042/bj1280353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bovarnick J. G., Chang S. W., Schiff J. A., Schwartzbach S. D. Events surrounding the early development of Euglena chloroplasts: experiments with streptomycin in non-dividing cells. J Gen Microbiol. 1974 Jul;83(0):51–62. doi: 10.1099/00221287-83-1-51. [DOI] [PubMed] [Google Scholar]
  4. Cahen D., Malkin S. Development and Repair of Photosystem II Activity in Normal and Chloramphenicol-treated Euglena gracilis Cells. Plant Physiol. 1978 Jul;62(1):1–5. doi: 10.1104/pp.62.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cahen D., Malkin S. Development of Photosystem II Complex during Greening of Chlamydomonas reinhardi y-1. Plant Physiol. 1976 Sep;58(3):257–267. doi: 10.1104/pp.58.3.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Diner B., Mauzerall D. Photosynthetic oxygen production and the size of the photosynthetic unit in a cellfree preparation from cyanidium caldarium. Biochim Biophys Acta. 1973 Jan 18;292(1):285–290. doi: 10.1016/0005-2728(73)90273-9. [DOI] [PubMed] [Google Scholar]
  7. Dubertret G., Ambard-Bretteville F. Functional and Structural Organization of Chlorophyll in the Developing Photosynthetic Membranes of Euglena gracilis Z: III. SEQUENCE OF EVENTS LEADING TO THE FORMATION OF FUNCTIONAL PHOTOSYSTEM II PHOTOSYNTHETIC UNITS. Plant Physiol. 1981 Jan;67(1):54–58. doi: 10.1104/pp.67.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dubertret G., Joliot P. Structure and organization of system II photosynthetic units during the greening of a dark-grown Chlorella mutant. Biochim Biophys Acta. 1974 Sep 20;357(3):399–411. doi: 10.1016/0005-2728(74)90030-9. [DOI] [PubMed] [Google Scholar]
  9. Dubertret G., Lefort-Tran M. Functional and structural organization of chlorophyll in the developing photosynthetic membranes of Euglena gracilis Z. II. Formation of system II photosynthetic units during greening under optimal light intensity. Biochim Biophys Acta. 1978 Aug 8;503(2):316–332. doi: 10.1016/0005-2728(78)90191-3. [DOI] [PubMed] [Google Scholar]
  10. Gurevitz M., Kratz H., Ohad I. Polypeptides of chloroplastic and cytoplastic origin required for development of photosystem II activity, and chlorophyll-protein complexes, in Euglena gracilis Z chloroplast membranes. Biochim Biophys Acta. 1977 Sep 14;461(3):475–488. doi: 10.1016/0005-2728(77)90234-1. [DOI] [PubMed] [Google Scholar]
  11. Heizmann P. La synthese des RNA ribosomiques au cours de l'eclairement d'Euglenes etiolees. Biochim Biophys Acta. 1974 Jul 11;353(3):301–312. [PubMed] [Google Scholar]
  12. Henningsen K. W., Boardman N. K. Development of Photochemical Activity and the Appearance of the High Potential Form of Cytochrome b-559 in Greening Barley Seedlings. Plant Physiol. 1973 Jun;51(6):1117–1126. doi: 10.1104/pp.51.6.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Herron H. A., Mauzerall D. The development of photosynthesis in a greening mutant of chlorella and an analysis of the light saturation curve. Plant Physiol. 1972 Jul;50(1):141–148. doi: 10.1104/pp.50.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Holowinsky A. W., Schiff J. A. Events surrounding the early development of Euglena chloroplasts. I. Induction by preillumination. Plant Physiol. 1970 Mar;45(3):339–347. doi: 10.1104/pp.45.3.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. JOLIOT A., JOLIOT P. ETUDE CIN'ETIQUE DE LA R'EACTION PHOTOCHIMIQUE LIB'ERANT L'OXYG'ENE AU COURS DE LA PHOTOSYNTH'ESE. C R Hebd Seances Acad Sci. 1964 May 4;258:4622–4625. [PubMed] [Google Scholar]
  16. Malkin S., Kok B. Fluorescence induction studies in isolated chloroplasts. I. Number of components involved in the reaction and quantum yields. Biochim Biophys Acta. 1966 Nov 8;126(3):413–432. doi: 10.1016/0926-6585(66)90001-x. [DOI] [PubMed] [Google Scholar]
  17. Melis A., Akoyunoglou G. Development of the Two Heterogeneous Photosystem II Units in Etiolated Bean Leaves. Plant Physiol. 1977 Jun;59(6):1156–1160. doi: 10.1104/pp.59.6.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Murata N., Nishimura M., Takamiya A. Fluorescene of chlorophyll in photosynthetic systems. II. Induction of fluorescence in isolated spinach chloroplasts. Biochim Biophys Acta. 1966 May 12;120(1):23–33. doi: 10.1016/0926-6585(66)90273-1. [DOI] [PubMed] [Google Scholar]
  19. Stern A. I., Epstein H. T., Schiff J. A. Studies of Chloroplast Development in Euglena. VI. Light Intensity as a Controlling Factor in Development. Plant Physiol. 1964 Mar;39(2):226–231. doi: 10.1104/pp.39.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]

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