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. 1981 Jan;67(1):85–88. doi: 10.1104/pp.67.1.85

Accumulation of Maltose during Photosynthesis in Protoplasts Isolated from Spinach Leaves Treated with Mannose 1

Alice Herold 1, Richard C Leegood 1, Philip H McNeil 1, Simon P Robinson 1,2
PMCID: PMC425626  PMID: 16661639

Abstract

When mannose was included in the enzyme incubation medium during the preparation of protoplasts from leaves of spinach, maltose was an early product of protoplast photosynthesis and, after 12 minutes, accounted for up to 15% of the 14C incorporated from 14CO2. Maltose was not detected in protoplasts prepared in the normal enzyme medium. Rapid separation of cytoplasm and chloroplasts following exposure to 14CO2 showed that maltose was present in both fractions. Direct measurements of [14C]maltose uptake indicated transport across the chloroplast envelope at rates similar to the transport of glucose. The source of maltose and site of its initial formation are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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