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. 1981 Jan;67(1):129–132. doi: 10.1104/pp.67.1.129

Kinetics of the Oxyhydrogen Reaction in the Presence and Absence of Carbon Dioxide in Scenedesmus obliquus1

David L Erbes 1,2, Martin Gibbs 1
PMCID: PMC425635  PMID: 16661612

Abstract

The oxyhydrogen reaction in the presence and absence of CO2 was studied in H2-adapted Scenedesmus obliquus by monitoring the initial rates of H2, O2, and 14CO2 uptake and the effect of inhibitors on these rates with gas-sensing electrodes and isotopic techniques. In the presence of 0.02 atmosphere O2, the pH2 was varied from 0 to 1 atmosphere. Whereas the rate of O2 uptake increased by only 30%, the rate of H2 uptake increased severalfold over the range of pH2 values. At 0.1 atmosphere H2 and 0.02 atmosphere O2, rates for H2 and O2 uptake were between 15 and 25 micromoles per milligram chlorophyll per hour. As the pH2 was changed from 0 to 1 atmosphere, the quotient H2:O2 changed from 0 to roughly 2. This change may reflect the competition between H2 and the endogenous respiratory electron donors. Respiration in the presence of glucose and acetate was also competitive with H2 uptake. KCN inhibited equally respiration (O2 uptake in the absence of H2) and the oxyhydrogen reaction in the presence and absence of CO2. The uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone accelerated the rate of respiration and the oxyhydrogen reaction to a similar extent. It was concluded that the oxyhydrogen reaction both in the presence and absence of CO2 has properties in common with components of respiration and photosynthesis. Participation of these two processes in the oxyhydrogen reaction would require a closely linked shuttle between mitochondrion and chloroplast.

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Selected References

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  1. Abeles F. B. Cell-free Hydrogenase from Chlamydomonas. Plant Physiol. 1964 Mar;39(2):169–176. doi: 10.1104/pp.39.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Erbes D. L., Burris R. H. The kinetics of methyl viologen oxidation and reduction by the hydrogenase from Clostridium pasteurianum. Biochim Biophys Acta. 1978 Jul 7;525(1):45–54. doi: 10.1016/0005-2744(78)90198-5. [DOI] [PubMed] [Google Scholar]
  3. Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grant N. G., Hommersand M. H. The Respiratory Chain of Chlorella protothecoides: I. Inhibitor Responses and Cytochrome Components of Whole Cells. Plant Physiol. 1974 Jul;54(1):50–56. doi: 10.1104/pp.54.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HORWITZ L. Observations on the oxyhydrogen reaction in Scenedesmus and its relation to respiration and photosynthesis. Arch Biochem Biophys. 1957 Jan;66(1):23–44. doi: 10.1016/0003-9861(57)90534-9. [DOI] [PubMed] [Google Scholar]
  6. Wang R., Healey F. P., Myers J. Amperometric measurement of hydrogen evolution in chlamydomonas. Plant Physiol. 1971 Jul;48(1):108–110. doi: 10.1104/pp.48.1.108. [DOI] [PMC free article] [PubMed] [Google Scholar]

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