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. 1981 Jan;67(1):150–155. doi: 10.1104/pp.67.1.150

Effect of Sugars and Amino Acids on Membrane Potential in Two Clones of Sugarcane 1

Sandra L Franz 1, Terry A Tattar 1
PMCID: PMC425640  PMID: 16661617

Abstract

Sugarcane (Saccharum officinarum L.) leaf parenchyma cells bathed in 1X solution maintained an average membrane potential of −135 millivolts in the dark. No difference in membrane potential was found between clones 51 NG 97 and H50 7209. An electrogenic pump appears to contribute to membrane potential in these cells. Sugars (25 millimolar) added externally caused the following membrane potential depolarizations (in millivolts) in clone 51 NG 97: glucose, 18 ± 4; galactose, 24 ± 7; 3-O-methylglucose, 10 ± 4; sucrose, 22 ± 3; fructose, 21 ± 7; raffinose, 9 ± 3; mannitol, 0; lactose, 0; melibiose, 0; and 1-O-methyl-α-galactose, 0. Glycine (25 millimolar) and serine (10 millimolar) caused depolarizations of 47 ± 7 and 23 ± 2 millivolts, respectively. Depolarization shows saturation kinetics with respect to glucose concentration, with a Km of 3 to 6 millimolar. The metabolic inhibitors KCN and salicyl hydroxamic acid together caused depolarization of the membrane potential and greatly inhibited depolarization by 25 millimolar glucose and 25 millimolar raffinose. In a series of substitution experiments, glucose (25 millimolar) caused almost total inhibition of depolarization by raffinose, sucrose, and 3-O-methylglucose (all 25 millimolar), but only partial inhibition of depolarization to 25 millimolar glycine. Glycine (25 millimolar), also, only partially inhibited depolarization by 25 millimolar glucose. Total depolarization to 25 millimolar glycine and 25 millimolar glucose was comparable to the amount of depolarization of membrane potential caused by 1 millimolar KCN plus 1 millimolar salicyl hydroxamic acid. The results are consistent with a co-transport mechanism of membrane transport, with sugars and amino acids being transported by separate carrier systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowen J. E. Sugar Transport in Immature Internodal Tissue of Sugarcane: II. Mechanism of Sucrose Transport. Plant Physiol. 1972 May;49(5):789–793. doi: 10.1104/pp.49.5.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bowen J. E. Sugar transport in immature internodal tissue of sugarcane: I. Mechanism and kinetics of accumulation. Plant Physiol. 1972 Jan;49(1):82–86. doi: 10.1104/pp.49.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Etherton B. Evidence for amino Acid-h co-transport in oat coleoptiles. Plant Physiol. 1978 Jun;61(6):933–937. doi: 10.1104/pp.61.6.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Etherton B. Relationship of Cell Transmembrane Electropotential to Potassium and Sodium Accumulation Ratios in Oat and Pea Seedlings. Plant Physiol. 1963 Sep;38(5):581–585. doi: 10.1104/pp.38.5.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gayler K. R., Glasziou K. T. Sugar Accumulation in Sugarcane: Carrier-mediated Active Transport of Glucose. Plant Physiol. 1972 Apr;49(4):563–568. doi: 10.1104/pp.49.4.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Maretzki A., Thom M. Arginine and lysine transport in sugarcane cell suspension cultures. Biochemistry. 1970 Jun 23;9(13):2731–2736. doi: 10.1021/bi00815a022. [DOI] [PubMed] [Google Scholar]
  7. Maretzki A., Thom M. Membrane transport of sugars in cell suspensions of sugarcane: I. Evidence for sites and specificity. Plant Physiol. 1972 Feb;49(2):177–182. doi: 10.1104/pp.49.2.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mitchell P. Translocations through natural membranes. Adv Enzymol Relat Areas Mol Biol. 1967;29:33–87. doi: 10.1002/9780470122747.ch2. [DOI] [PubMed] [Google Scholar]
  9. Sacher J. A., Hatch M. D., Glasziou K. T. Sugar Accumulation Cycle in Sugar Cane. III. Physical & Metabolic Aspects of Cycle in Immature Storage Tissues. Plant Physiol. 1963 May;38(3):348–354. doi: 10.1104/pp.38.3.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Strobel G. A. The toxin-binding protein of sugarcane, its role in the plant and in disease development. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4232–4236. doi: 10.1073/pnas.71.10.4232. [DOI] [PMC free article] [PubMed] [Google Scholar]

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