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. 1981 Feb;67(2):216–220. doi: 10.1104/pp.67.2.216

Transition of Lipid Synthesis from Chloroplasts to a Cytoplasmic System during Hardening in Chlorella ellipsoidea1,2

Shoji Hatano 1, Kiyotaka Kabata 1, Haruo Sadakane 1
PMCID: PMC425657  PMID: 16661649

Abstract

Chlorella ellipsoidea Gerneck (IAM C-27) was synchronously grown and cells at an intermediate stage in the ripening phase of the cell cycle were hardened at 3 C for 48 hours. At various times of hardening, the cells were pulse-labeled for 4 minutes with [14C]NaHCO3 in the light or with [14C]glucose in the dark, and the incorporation rate of 14C into total lipids was determined. A high incorporation rate of [14C]NaHCO3 at zero time of hardening decreased after 6 hours. In the next 15 hours, a distinct increase was noted. This increase occurred prior to the development of frost hardiness. Cycloheximide completely inhibited both the increase and the development, and 3-(3,4-dichlorophenyl)-1,1-dimethylurea remarkably lowered the high incorporation rate at zero time. The incorporation rate of [14C]glucose increased along with hardiness in the dark. These results suggest that the major site of lipid synthesis shifts from chloroplasts to a cytoplasmic system during hardening of Chlorella.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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