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. 1981 Feb;67(2):246–249. doi: 10.1104/pp.67.2.246

Properties of Magnesium Chelatase in Greening Etioplasts

METAL ION SPECIFICITY AND EFFECT OF SUBSTRATE CONCENTRATIONS 1

Thomas P Fuesler 1, Lawrence A Wright Jr 1,2, Paul A Castelfranco 1,3
PMCID: PMC425663  PMID: 16661655

Abstract

Evidence was obtained by means of low temperature spectrofluorimetry and high pressure liquid chromatography for the exclusive chelation of Mg2+ into protoporphyrin IX by a plastid preparation extracted from greening cucumber cotyledons. Under the experimental conditions which were optimized for Mg2+ chelation, Zn2+ chelation was not detected. However, Zn2+ chelation was observed when ATP was omitted and ZnCl2 was added to the incubation mixture. The observed Zn2+ chelation was partially nonenzymic. The requirements for the enzyme, Mg chelatase, are discussed in terms of the regulatory function of this enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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