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. 1981 Feb;67(2):246–249. doi: 10.1104/pp.67.2.246

Properties of Magnesium Chelatase in Greening Etioplasts

METAL ION SPECIFICITY AND EFFECT OF SUBSTRATE CONCENTRATIONS 1

Thomas P Fuesler 1, Lawrence A Wright Jr 1,2, Paul A Castelfranco 1,3
PMCID: PMC425663  PMID: 16661655

Abstract

Evidence was obtained by means of low temperature spectrofluorimetry and high pressure liquid chromatography for the exclusive chelation of Mg2+ into protoporphyrin IX by a plastid preparation extracted from greening cucumber cotyledons. Under the experimental conditions which were optimized for Mg2+ chelation, Zn2+ chelation was not detected. However, Zn2+ chelation was observed when ATP was omitted and ZnCl2 was added to the incubation mixture. The observed Zn2+ chelation was partially nonenzymic. The requirements for the enzyme, Mg chelatase, are discussed in terms of the regulatory function of this enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ardao C., Vennesland B. Chlorophyllase Activity of Spinach Chloroplastin. Plant Physiol. 1960 May;35(3):368–371. doi: 10.1104/pp.35.3.368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Castelfranco P. A., Weinstein J. D., Schwarcz S., Pardo A. D., Wezelman B. E. The Mg insertion step in chlorophyll biosynthesis. Arch Biochem Biophys. 1979 Feb;192(2):592–598. doi: 10.1016/0003-9861(79)90130-9. [DOI] [PubMed] [Google Scholar]
  3. Lamola A. A., Yamane T. Zinc protoporphyrin in the erythrocytes of patients with lead intoxication and iron deficiency anemia. Science. 1974 Dec 6;186(4167):936–938. doi: 10.1126/science.186.4167.936. [DOI] [PubMed] [Google Scholar]
  4. Neuberger A., Tait G. H. Studies on the biosynthesis of porphyrin and bacteriochlorophyll by Rhodopseudomonas spheroides. 5. Zinc-protoporphyrin chelatase. Biochem J. 1964 Mar;90(3):607–616. doi: 10.1042/bj0900607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ohaniance L., Chaix P. Effet inhibiteur de Zn2+ sur la biosynthèse induite par l'oxygène des enzymes respiratoires de la levure. Biochim Biophys Acta. 1966 Nov 15;128(2):228–238. [PubMed] [Google Scholar]
  6. Pardo A. D., Chereskin B. M., Castelfranco P. A., Franceschi V. R., Wezelman B. E. ATP requirement for mg chelatase in developing chloroplasts. Plant Physiol. 1980 May;65(5):956–960. doi: 10.1104/pp.65.5.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rebeiz C. Z., Smith B. B., Mattheis J. R., Rebeiz C. C., Dayton D. F. Chloroplast biogenesis. Biosynthesis and accumulation of Mg-protoporphyrin IX monoester and other metalloporphyrins by isolated etioplasts and developing chloroplasts. Arch Biochem Biophys. 1975 Mar;167(1):351–365. doi: 10.1016/0003-9861(75)90471-3. [DOI] [PubMed] [Google Scholar]

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