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. 2014 Oct 2;198(4):1447–1456. doi: 10.1534/genetics.114.168732

Figure 7.

Figure 7

OsMSH4 affects the formation of HEI10 prominent foci. (A and C) Staining for HEI10 at zygotene in wild type and Osmsh4-1. (B and D) Staining for HEI10 at late pachytene in wild type and Osmsh4-1. Bars, 5 μm. (E) HEI10 interacted with OsMSH5 in yeast two-hybrid assays. The interactions were verified by the growth of yeast on selection medium SD/QDO/X/A. The blue dots from coexpression of OsMSH4 and OsMSH5 were used as positive control. (F) HEI10 did not interact with OsMSH4 in yeast two-hybrid assays. (G) OsMSH5 bound to HEI10 in GST pull-down assays. Purified GST–OsMSH5 protein was used as positive control (input). The result of MBP–HEI10 incubated with GST tag was used as the negative control. Glutathione Sepharose beads coated with GST-OsMSH5 were used to capture either MBP tag or MBP–HEI10. GST–OsMSH5 specifically bound to HEI10. (H) Statistical contrast of HEI10 prominent foci between wild type and Osmsh4-1.