Figure 3.

The presence of a Cdc28 or Hrr25 protein kinase focus was correlated with cell viability. Cells containing the indicated GFP fusion proteins were grown for 1 or 6 days in SC minimal medium and then stained with PI as described in Materials and Methods. PI preferentially stains dying and dead cells in the culture. Pbp1 is a reporter for stress granules and Dcp2 for P-bodies. The proteins examined were as follows: (A) Pbp1, (B) Dcp2, (C) Cdc28, and (D) Hrr25.