Figure 5.

Four protein kinases were associated preferentially with P-bodies in stationary-phase cells. (A and B) The colocalization of the four GFP-tagged protein kinases, Hrr25, Ypk3, Tpk3, and Cdc15, with a reporter for P-bodies (Edc3-mCh) and stress granules (Pbp1-mCh). (C) Quantitation of the colocalization data. (D and E) The presence of the hyperactive RAS2^val19 allele resulted in diminished numbers of protein kinase foci. Note that this analysis was performed in minimal medium to maintain selection for the RAS2^val19 plasmid. The values shown in each graph represent the average of at least two independent experiments (n = 100).