Figure 2.

(A–D) Remating rate (number of females remating in 1 hr) at 24 or 48 hr following initial matings with either SP-transferring (SP⁺, bars with light shading) or SP-lacking (SP⁰, bars with dark shading) males. Simultaneously, the mating rate of age- and genotype-matched virgin females (bars with intermediate shading) was measured for comparison. (A and B) Remating in females hypomorphic for mir-279 and mir-317 in the w^[Dah] or w^[CS] genetic backgrounds, respectively. (C) Receptivity of mir-278 knockout females in the w^[Dah] genetic background and (D) receptivity of mir-184 knockout females in the w¹¹¹⁸ background. (E–H) Effect size (SP⁰ − SP⁺ treatment) and 95% CI for female remating rate for the different female genotypes mated first to a SP⁺ or SP⁰ male and provided with an opportunity to remate with a Dahomey wild-type male either 24 (diamonds with dark shading) or 48 hr (squares with light shading) after a first mating. Note that in the tests of the mir-184 knockout, (Figure 2H) all the control w¹¹¹⁸ females remated; therefore, Cohen’s d could not be calculated. Hence only the effect size for mir-184 is shown.