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. 1981 Mar;67(3):408–414. doi: 10.1104/pp.67.3.408

Eyespot Disease of Sugarcane 1

INDUCTION OF HOST-SPECIFIC TOXIN AND ITS INTERACTION WITH LEAF CELLS

Philip J Larkin 1, William R Scowcroft 1
PMCID: PMC425695  PMID: 16661684

Abstract

Helminthosporium sacchari produces a toxin which is responsible for the symptoms of eyespot disease in Saccharum officinarum. A rapid and highly repeatable bioassay based on increase in conductivity of tissue leachates showed that the interaction of toxin with sugarcane obeys Michaelis-Menten hyperbolic saturation kinetics. There was no evidence for positive or negative cooperation interaction. Resistant and susceptible cultivars of sugar cane had distinctive conductivity characteristics. Co-cultures of H. sacchari and suspension cultures of sugarcane gave up to a 4,000-fold increase in toxin production.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Byther R. S., Steiner G. W. Heat-induced Resistance of Sugarcane to Helminthosporium sacchari and Helminthosporoside. Plant Physiol. 1975 Sep;56(3):415–419. doi: 10.1104/pp.56.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Pinkerton F., Strobel G. Serinol as an activator of toxin production in attenuated cultures of Helminthosporium sacchari. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4007–4011. doi: 10.1073/pnas.73.11.4007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Steiner G. W., Strobel G. A. Helminthosporoside, a host-specific toxin from Helminthosporium sacchari. J Biol Chem. 1971 Jul 10;246(13):4350–4357. [PubMed] [Google Scholar]
  4. Strobel G. A. Biochemical basis of the resistance of sugarcane to eyespot disease. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1693–1696. doi: 10.1073/pnas.70.6.1693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Strobel G. A., Hapner K. D. Transfer of toxin susceptibility to plant protoplasts via the hemlmintosporoside binding protein of sugarcane. Biochem Biophys Res Commun. 1975 Apr 21;63(4):1151–1156. doi: 10.1016/0006-291x(75)90689-0. [DOI] [PubMed] [Google Scholar]
  6. Strobel G. A., Hess W. M. Evidence for the presence of the toxin-binding protein on the plasma membrane of sugarcane cells. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1413–1417. doi: 10.1073/pnas.71.4.1413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Strobel G. A., Steiner G. W., Byther R. Deficiency of toxin-binding protein activity in mutants of sugarcane clone H54-775 as it relates to disease resistance. Biochem Genet. 1975 Oct;13(9-10):557–565. doi: 10.1007/BF00484915. [DOI] [PubMed] [Google Scholar]
  8. Strobel G. A. The helminthosporoside-binding protein of sugarcane. Its properties and relationship to susceptibility to the eye spot disease. J Biol Chem. 1973 Feb 25;248(4):1321–1328. [PubMed] [Google Scholar]
  9. Strobel G. A. The relationship between membrane ATPase activity in sugarcane and heat-induced resistance to helminthosporoside. Biochim Biophys Acta. 1979 Jul 5;554(2):460–468. doi: 10.1016/0005-2736(79)90384-5. [DOI] [PubMed] [Google Scholar]

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