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. 1981 Mar;67(3):470–473. doi: 10.1104/pp.67.3.470

Slow Passive Diffusion of Orthophosphate between Intact Isolated Chloroplasts and Suspending Medium 1

Gilles Mourioux 1, Roland Douce 1
PMCID: PMC425707  PMID: 16661696

Abstract

Isolated spinach chloroplasts purified by isopycnic centrifugation in density gradients of Percoll were found to be highly intact, to be devoid of extrachloroplastic contaminations, and to retain a high rate of CO2-dependent O2 evolution.

When suspended in a medium which avoided rupture of the envelope, intact purified chloroplasts progressively lost their phosphate content by passive diffusion. This led to a slow decrease in the uptake of labeled 3-phosphoglyceric acid or orthophosphate (Pi) and in the rate of CO2-dependent O2 evolution by isolated chloroplasts. Under these conditions, there was a good correlation between the rate of CO2-dependent O2 evolution and the concentration of Pi in the stroma space. Addition of Pi to the suspending medium at a final concentration of 10 millimolar, which counterpoised the slow efflux of Pi from the chloroplasts, slowed considerably the decrease in the rate of CO2-dependent O2 evolution.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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