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. 1981 Apr;67(4):701–704. doi: 10.1104/pp.67.4.701

Inhibition of 2,4-Dichlorophenoxyacetic Acid Conjugation to Amino Acids by Treatment of Cultured Soybean Cells with Cytokinins

Michael J Montague 1, Russel K Enns 1, Ned R Siegel 1, Ernest G Jaworski 1
PMCID: PMC425757  PMID: 16661739

Abstract

Kinetin, and all other cytokinins tested, inhibited the conjugation of [14C]2,4-dichlorophenoxyacetic acid (2, 4-D) to amino acids when supplied simultaneously with the 2,4-D to cultured soybean cells. Upon transfer to hormone-free medium, the cytokinin-treated cells released more of their [14C]2,4-D than did the control cells. Initial exposure to low 2,4-D and high kinetin levels resulted in the greatest release of 2,4-D upon subsequent transfer. The observed alteration in 2,4-D metabolism did not seem to be correlated with growth rate. Appropriate treatment of soybean cells with kinetin resulted in 2,4-D metabolism that resembled the 2,4-D metabolism of embryogenic carrot cells. However, no new morphological structures were observed in these soybean cultures, indicating that other factors are related to the failure of soybean cells to regenerate in culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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