Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1981 Apr;67(4):711–715. doi: 10.1104/pp.67.4.711

Effect of Temperature and BASF 13 338 on the Lipid Composition and Respiration of Wheat Roots

Edward N Ashworth 1,2,3,4,5,1, Meryl N Christiansen 1,2,3,4,5, Judith B St John 1,2,3,4,5, Glenn W Patterson 1,2,3,4,5
PMCID: PMC425759  PMID: 16661741

Abstract

The fatty acid composition of wheat seedling roots changed in response to temperature. As temperature declined, the level of linolenic acid increased and the level of linoleic acid decreased. The distribution of phospholipid classes was not influenced by temperature. Phosphatidyl choline and phosphatidyl ethanolamine were the predominant phospholipids isolated and comprised 85% of the total lipid phosphorus. Smaller quantities of phosphatidyl glycerol, phosphatidyl inositol, phosphatidic acid, and phosphatidyl serine were isolated. The fatty acid composition of phosphatidyl choline and phosphatidyl ethanolamine were the same and temperature affected the fatty acid composition of both phospholipids in the same manner.

Growth in the presence of the substituted pyridazinone, BASF 13 338 (4-chloro-5-dimethylamino-2-phenyl-3(2H)pyridazinone), reduced the level of linolenic acid and increased the level of linoleic acid in the phosphatidyl choline, phosphatidyl ethanolamine, and total polar lipid fractions. BASF 13 338 did not affect the levels of palmitate, stearate, and oleate or the distribution of phospholipid classes.

Respiration rates of wheat root tips were measured over a range of temperatures. The respiration rate declined as the temperature decreased. Neither the temperature at which the tissue was grown nor BASF 13 338 treatment influenced the ability of root tips to respire at any temperature from 4 to 30 C. The results indicated that the relative proportion of linolenic acid to linoleic acid did not influence the plants ability to grow and respire over the range of temperatures tested.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. Deyoe D. R. Glycerolipid and Fatty Acid Changes in Eastern White Pine Chloroplast Lamellae during the Onset of Winter. Plant Physiol. 1979 Dec;64(6):924–929. doi: 10.1104/pp.64.6.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dogras C. C., Dilley D. R., Herner R. C. Phospholipid Biosynthesis and Fatty Acid Content in Relation to Chilling Injury during Germination of Seeds. Plant Physiol. 1977 Dec;60(6):897–902. doi: 10.1104/pp.60.6.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. Hax W. M., van Kessel W. S. High-performance liquid chromatographic separation and photometric detection of phospholipids. J Chromatogr. 1977 Nov 11;142:735–741. doi: 10.1016/s0021-9673(01)92081-3. [DOI] [PubMed] [Google Scholar]
  6. John J. B. Manipulation of galactolipid Fatty Acid composition with substituted pyridazinones. Plant Physiol. 1976 Jan;57(1):38–40. doi: 10.1104/pp.57.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ladbrooke B. D., Chapman D. Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies. Chem Phys Lipids. 1969 Dec;3(4):304–356. doi: 10.1016/0009-3084(69)90040-1. [DOI] [PubMed] [Google Scholar]
  8. Lyons J. M., Raison J. K. Oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury. Plant Physiol. 1970 Apr;45(4):386–389. doi: 10.1104/pp.45.4.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller R. W., de la Roche I., Pomeroy M. K. Structural and functional responses of wheat mitochondrial membranes to growth at low temperatures. Plant Physiol. 1974 Mar;53(3):426–433. doi: 10.1104/pp.53.3.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pomeroy M. K., Andrews C. J. Effect of Temperature on Respiration of Mitochondria and Shoot Segments from Cold hardened and Nonhardened Wheat and Rye Seedlings. Plant Physiol. 1975 Nov;56(5):703–706. doi: 10.1104/pp.56.5.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Raison J. K., Chapman E. A., White P. Y. Wheat mitochondria: oxidative activity and membrane lipid structure as a function of temperature. Plant Physiol. 1977 Apr;59(4):623–627. doi: 10.1104/pp.59.4.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thomson L. W., Zalik S. Lipids in rye seedlings in relation to vernalization. Plant Physiol. 1973 Sep;52(3):268–273. doi: 10.1104/pp.52.3.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Willemot C., Hope H. J., Williams R. J., Michaud R. Changes in fatty acid composition of winter wheat during frost hardening. Cryobiology. 1977 Feb;14(1):87–93. doi: 10.1016/0011-2240(77)90126-2. [DOI] [PubMed] [Google Scholar]
  14. Willemot C. Simultaneous Inhibition of Linolenic Acid Synthesis in Winter Wheat Roots and Frost Hardening by BASF 13-338, a Derivative of Pyridazinone. Plant Physiol. 1977 Jul;60(1):1–4. doi: 10.1104/pp.60.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Willemot C. Stimulation of Phospholipid Biosynthesis during Frost Hardening of Winter Wheat. Plant Physiol. 1975 Feb;55(2):356–359. doi: 10.1104/pp.55.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. de la Roche A. I. Increase in linolenic Acid is not a prerequisite for development of freezing tolerance in wheat. Plant Physiol. 1979 Jan;63(1):5–8. doi: 10.1104/pp.63.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. de la Roche I. A., Andrews C. J. Changes in Phospholipid Composition of a Winter Wheat Cultivar during Germination at 2 C and 24 C. Plant Physiol. 1973 Mar;51(3):468–473. doi: 10.1104/pp.51.3.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. de la Roche I. A., Pomeroy M. K., Andrews C. J. Changes in fatty acid composition in wheat cultivars of contrasting hardiness. Cryobiology. 1975 Oct;12(5):506–512. doi: 10.1016/0011-2240(75)90032-2. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES