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. 1981 May;67(5):882–886. doi: 10.1104/pp.67.5.882

Tunicamycin Inhibits Protein Glycosylation in Suspension Cultured Soybean Cells 1

Hidetaka Hori 1, Alan D Elbein 1
PMCID: PMC425794  PMID: 16661786

Abstract

Soybean cells in suspension culture incorporate [3H]mannose into dolichyl-phosphoryl-mannose and into lipid-linked oligosaccharides as well as into extracellular and cell wall macromolecules. Tunicamycin completely inhibited the formation of lipid-linked oligosaccharides at a concentration of 5 to 10 micrograms per milliliter, but it had no effect on the formation of dolichyl-phosphoryl-mannose. Tunicamycin did inhibit the incorporation of [3H]mannose into cell wall components and extracellular macromolecules, but even at 20 micrograms per milliliter of antibiotic there was still about 30% incorporation of mannose. The radioactivity in these macromolecules was localized in mannose (70%), rhamnose (20%), galactose (8%), and fucose (2%) in the absence of antibiotic. But when tunicamycin was added, very little radioactive mannose was found in cell wall or extracellular components. The incorporation of [3H]leucine into membrane components and [14C]proline into cell wall components by these suspension cultures was unaffected by tunicamycin. However, tunicamycin did inhibit the appearance of leucine-labeled extracellular macromolecules, probably because it prevented their secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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