Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1981 Jun;67(6):1181–1184. doi: 10.1104/pp.67.6.1181

Cytokinin-Active Ribonucleosides in Phaseolus RNA

I. IDENTIFICATION IN tRNA FROM ETIOLATED PHASEOLUS VULGARIS L. SEEDLINGS 1

Cynthia A Edwards 1,2,2, Donald J Armstrong 1,2,3, Richard W Kaiss-Chapman 1,2,4, Roy O Morris 1,2
PMCID: PMC425857  PMID: 16661832

Abstract

The cytokinin-active ribonucleosides present in tRNA from etiolated Phaseolus vulgaris L. seedlings have been isolated and identified as cis-ribosylzeatin, 2-methylthio-ribosylzeatin, and N6-(Δ2-isopentenyl)-adenosine. The structures of the compounds were established on the basis of their chromatographic properties and the mass spectra of their permethylated and perdeuteromethylated derivatives. Cis-ribosylzeatin was the major cytokinin-active constituent of tRNA from this source.

Full text

PDF
1181

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. J., Burrows W. J., Evans P. K., Skoog F. Isolation of cytokinins from tRNA. Biochem Biophys Res Commun. 1969 Oct 22;37(3):451–456. doi: 10.1016/0006-291x(69)90936-x. [DOI] [PubMed] [Google Scholar]
  2. Armstrong D. J., Skoog F. Chromatography of Cytokinins on a Neutral Polystyrene Resin: A Simple Procedure for the Separation of the cis and trans Isomers of Zeatin or Ribosylzeatin. Plant Physiol. 1975 Feb;55(2):237–239. doi: 10.1104/pp.55.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Armstrong D. J., Skoog F., Kirkegaard L. H., Hampel A. E., Bock R. M., Gillam I., Tener G. M. Cytokinins: distribution in species of yeast transfer RNA. Proc Natl Acad Sci U S A. 1969 Jun;63(2):504–511. doi: 10.1073/pnas.63.2.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burrows W. J., Armstrong D. J., Kaminek M., Skoog F., Bock R. M., Hecht S. M., Dammann L. G., Leonard N. J., Occolowitz J. Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid. Biochemistry. 1970 Apr 28;9(9):1867–1872. doi: 10.1021/bi00811a001. [DOI] [PubMed] [Google Scholar]
  5. Burrows W. J., Skoog F., Leonard N. J. Isolation and identification of cytokinins located in the transfer ribonucleic acid of tobacco callus grown in the presence of 6-benzylaminopurine. Biochemistry. 1971 Jun 8;10(12):2189–2194. doi: 10.1021/bi00788a002. [DOI] [PubMed] [Google Scholar]
  6. Chang S. H., Brum C. K., Siberklang M., RajBhandary U. L., Hecker L. I., Barnett W. E. The first nucleotide sequence of an organelle transfer RNA: chloroplastic tRNAphe. Cell. 1976 Dec;9(4 Pt 2):717–723. doi: 10.1016/0092-8674(76)90135-5. [DOI] [PubMed] [Google Scholar]
  7. Chapman R. W., Morris R. O., Zaerr J. B. Occurrence of trans-ribosylzeatin in Agrobacterium tumefaciens tRNA. Nature. 1976 Jul 8;262(5564):153–154. doi: 10.1038/262153a0. [DOI] [PubMed] [Google Scholar]
  8. Guillemaut P., Keith G. Primary structure of bean chloroplastic tRNAPhe. Comparison with Euglena chloroplastic tRNAPhe. FEBS Lett. 1977 Dec 15;84(2):351–356. doi: 10.1016/0014-5793(77)80723-0. [DOI] [PubMed] [Google Scholar]
  9. Guillemaut P., Martin R., Weil J. H. Purification and base composition of a chloroplastic tRANphe from Phaseolus vulgaris. FEBS Lett. 1976 Apr 1;63(2):273–277. doi: 10.1016/0014-5793(76)80110-x. [DOI] [PubMed] [Google Scholar]
  10. HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
  11. Hall R. H., Csonka L., David H., McLennan B. Cytokinins in the soluble RNA of plant tissues. Science. 1967 Apr 7;156(3771):69–71. doi: 10.1126/science.156.3771.69. [DOI] [PubMed] [Google Scholar]
  12. Hall R. H. N6-(delta 2-isopentenyl)adenosine: chemical reactions, biosynthesis, metabolism, and significance to the structure and function of tRNA. Prog Nucleic Acid Res Mol Biol. 1970;10:57–86. doi: 10.1016/s0079-6603(08)60561-9. [DOI] [PubMed] [Google Scholar]
  13. Hecker L. I., Uziel M., Barnett W. E. Comparative base compositions of chloroplast and cytoplasmic tRNAPhe's from Euglena gracilis. Nucleic Acids Res. 1976 Feb;3(2):371–380. doi: 10.1093/nar/3.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaiss-Chapman R. W., Morris R. O. Trans-zeatin in culture filtrates of Agrobacterium tumefaciens. Biochem Biophys Res Commun. 1976 May 23;76(2):453–459. doi: 10.1016/0006-291x(77)90746-x. [DOI] [PubMed] [Google Scholar]
  15. Murai N., Armstrong D. J., Skoog F. Incorporation of mevalonic Acid into ribosylzeatin in tobacco callus ribonucleic Acid preparations. Plant Physiol. 1975 May;55(5):853–858. doi: 10.1104/pp.55.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  17. RALPH R. K., BELLAMY A. R. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964 May 18;87:9–16. doi: 10.1016/0926-6550(64)90041-6. [DOI] [PubMed] [Google Scholar]
  18. Sondheimer E., Tzou D. S. The Metabolism of Hormones during Seed Germination and Dormancy: II. The Metabolism of 8-C-Zeatin in Bean Axes. Plant Physiol. 1971 Apr;47(4):516–520. doi: 10.1104/pp.47.4.516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Swaminathan S., Bock R. M. Subcellular localization of cytokinins in transfer ribonucleic Acid. Plant Physiol. 1977 Apr;59(4):558–563. doi: 10.1104/pp.59.4.558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vreman H. J., Skoog F. Cytokinins in pisum transfer ribonucleic Acid. Plant Physiol. 1972 May;49(5):848–851. doi: 10.1104/pp.49.5.848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vreman H. J., Thomas R., Corse J. Cytokinins in tRNA Obtained from Spinacia oleracea L. Leaves and Isolated Chloroplasts. Plant Physiol. 1978 Feb;61(2):296–306. doi: 10.1104/pp.61.2.296. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES