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. 1981 Jun;67(6):1214–1219. doi: 10.1104/pp.67.6.1214

Measurement of Protein Degradation in Leaves of Zea mays Using [3H]Acetic Anhydride and Tritiated Water

Ellen Simpson 1,2,1, Robert J Cooke 1,2,2, David D Davies 1,2,2
PMCID: PMC425864  PMID: 16661839

Abstract

The rate of protein degradation in Zea mays leaves has been estimated by using tritiated water and [3H]acetic anhydride as the labeling agents. Both methods circumvent many of the problems usually associated with measuring protein degradation in plants. The half-life of ribulose-1,5-bisphosphate carboxylase protein in second leaves of 13-day-old seedlings under continuous light was found to be 7.8 ± 0.9 days by the tritiated water technique and 6.5 ± 0.8 days by the [3H]acetic anhydride method. The half-lives determined under a 14-hour-light, 10-hour-dark photoperiod are 6.2 ± 0.8 days with tritiated water and 5.4 ± 0.4 days with [3H]acetic anhydride. Whereas the values obtained by the two methods do not differ significantly, the use of either method for the determination of protein half-life can be recommended.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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