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. 1981 Jul;68(1):158–164. doi: 10.1104/pp.68.1.158

pH-Dependent Interactions between Pea Cell Wall Polymers Possibly Involved in Wall Deposition and Growth 1

George W Bates 1,2, Peter M Ray 1
PMCID: PMC425908  PMID: 16661862

Abstract

In an effort to detect a pH-dependent release of polymers such as xyloglucans, thought to be involved in auxin-induced cell wall expansion during growth, radioactively labeled cell walls from pea stem tissue were incubated at different pH values, and changes in water-soluble, ethanol- or trichloroacetic acid-insoluble components were determined. This revealed the occurrence, at neutral pH, of a time- and pH-dependent binding of soluble pectin, in the walls, to a heat-labile, presumably protein, wall component, yielding a trichloroacetic acid-insoluble pectin-protein complex. This reaction, which can also be observed between polymers in water extracts of cell walls, is inhibited at low pH and by Ca2+, and appears to be of a physical, possibly lectin-like, nature. Progressive binding of pectin or of the pectin-protein complex to the insoluble wall structure is also observed. These reactions may be involved in wall assembly during its deposition, and may participate in, or be analogous to pH-dependent physical interactions that participate in, wall extension during cell growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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