Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1981 Jul;68(1):180–186. doi: 10.1104/pp.68.1.180

Developmental Biochemistry of Cottonseed Embryogenesis and Germination 1

XII. PURIFICATION AND PROPERTIES OF PRINCIPAL STORAGE PROTEINS

Leon Dure III 1, Caryl Chlan 1
PMCID: PMC425912  PMID: 16661867

Abstract

The two principal storage proteins of the cottonseed have been purified and partially characterized. They have apparent molecular weights of 52,000 and 48,000 and are both comprised of sets of isoelectric isomers. Both sets have nearly identical solubility properties, ultraviolet absorption spectra, and contain similar proportions of amino acid residues. Furthermore, both sets share antigenic determinants suggesting their origin from a common ancestral gene. However, only the 52,000 set appears to be glycosylated.

Full text

PDF
180

Images in this article

181Image
on p.181
181Image
on p.181
182Image
on p.182
183Image
on p.183
184Image
on p.184
185Image
on p.185

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Dure L., Galau G. A. Developmental Biochemistry of Cottonseed Embryogenesis and Germination : XIII. REGULATION OF BIOSYNTHESIS OF PRINCIPAL STORAGE PROTEINS. Plant Physiol. 1981 Jul;68(1):187–194. doi: 10.1104/pp.68.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Foster J. A., Rich C. B., Fletcher S., Karr S. R., Przybyla A. Translation of chick aortic elastin messenger ribonucleic acid. Comparison to elastin synthesis in chick aorta organ culture. Biochemistry. 1980 Mar 4;19(5):857–864. doi: 10.1021/bi00546a005. [DOI] [PubMed] [Google Scholar]
  4. GOA J. A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid. Scand J Clin Lab Invest. 1953;5(3):218–222. doi: 10.3109/00365515309094189. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  8. Robinson A. B. Evolution and the distribution of glutaminyl and asparaginyl residues in proteins. Proc Natl Acad Sci U S A. 1974 Mar;71(3):885–888. doi: 10.1073/pnas.71.3.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Walbot V., Dure L. S., 3rd Developmental biochemistry of cotton seed embryogenesis and germination. VII. Characterization of the cotton genome. J Mol Biol. 1976 Mar 15;101(4):503–536. doi: 10.1016/0022-2836(76)90242-4. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES