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. 2014 Sep 3;5(20):9783–9797. doi: 10.18632/oncotarget.2392

Figure 3. PGR promoter methylation represses PR expression in serous endometrial cancer cells.

Figure 3

(A) Bisulfite sequencing. PGR promoter methylation was quantified in Ishikawa H and Hec50co cells before or after hypomethylating agent 5-aza-decitabine (5-aza-dC) treatment. (B) q-PCR analysis. Hec50co cells were treated with DMSO (vehicle control), 100 nM 5-aza-dC for 5 days with fresh 5-aza-dC added every other day, 5-aza-dC for 5 days +100 nM P4 for 4h. mRNA expression of PGR, AREG and PAEP was measured by q-PCR and normalized to 18S, and data are displayed as fold-change relative to DMSO control. Comparisons of normalized expression values (ΔCt) employed the conventional ΔΔCt fold change method. (C) Representative immunofluorescent image showing PR expression in Hec50co cells treated with DMSO control, 5 days of 100 nM 5-aza-dC, 100 nM P4, or both. Scale bar = 50 μm.