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. 1981 Sep;68(3):616–618. doi: 10.1104/pp.68.3.616

A Sycamore Cell Wall Polysaccharide and a Chemically Related Tomato Leaf Polysaccharide Possess Similar Proteinase Inhibitor-Inducing Activities 1

Clarence A Ryan 1,2,2, Paul Bishop 1,2, Gregory Pearce 1,2, Alan G Darvill 1,2, Michael McNeil 1,2, Peter Albersheim 1,2
PMCID: PMC425949  PMID: 16661967

Abstract

A large pectic polysaccharide, called rhamnogalacturonan I, that is solubilized by a fungal endo-α-1,4-polygalacturonase from the purified walls of suspension-cultured sycamore cells possesses proteinase inhibitor-inducing activity similar to that of the proteinase inhibitor-inducing factor, a pectic-like oligosaccharide fraction isolated from tomato leaves. This suggests that the proteinase inhibitor-inducing activity resides in particular polysaccharide fragments which can be released when plant cell walls are exposed to appropriate enzyme degradation as a result of either wounding or pest attack.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baig M. M., Cerda J. J. Use of galactose oxidase tritiated-potassium borohydride method for labeling pectic polysaccharides. Anal Biochem. 1979 Oct 1;98(2):429–432. doi: 10.1016/0003-2697(79)90162-3. [DOI] [PubMed] [Google Scholar]
  2. Bauer W. D., Talmadge K. W., Keegstra K., Albersheim P. The Structure of Plant Cell Walls: II. The Hemicellulose of the Walls of Suspension-cultured Sycamore Cells. Plant Physiol. 1973 Jan;51(1):174–187. doi: 10.1104/pp.51.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
  4. Darvill A. G., McNeil M., Albersheim P. Structure of Plant Cell Walls: VIII. A New Pectic Polysaccharide. Plant Physiol. 1978 Sep;62(3):418–422. doi: 10.1104/pp.62.3.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. English P. D., Maglothin A., Keegstra K., Albersheim P. A Cell Wall-degrading Endopolygalacturonase Secreted by Colletotrichum lindemuthianum. Plant Physiol. 1972 Mar;49(3):293–298. doi: 10.1104/pp.49.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green T. R., Ryan C. A. Wound-induced Proteinase Inhibitor in Tomato Leaves: Some Effects of Light and Temperature on the Wound Response. Plant Physiol. 1973 Jan;51(1):19–21. doi: 10.1104/pp.51.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gustafson G., Ryan C. A. Specificity of protein turnover in tomato leaves. Accumulation of proteinase inhibitors, induced with the wound hormone, PIIF. J Biol Chem. 1976 Nov 25;251(22):7004–7010. [PubMed] [Google Scholar]
  8. McFarland D., Ryan C. A. Proteinase Inhibitor-inducing Factor in Plant Leaves: A Phylogenetic Survey. Plant Physiol. 1974 Nov;54(5):706–708. doi: 10.1104/pp.54.5.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McNeil M., Darvill A. G., Albersheim P. Structure of Plant Cell Walls: X. RHAMNOGALACTURONAN I, A STRUCTURALLY COMPLEX PECTIC POLYSACCHARIDE IN THE WALLS OF SUSPENSION-CULTURED SYCAMORE CELLS. Plant Physiol. 1980 Dec;66(6):1128–1134. doi: 10.1104/pp.66.6.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ryan C. A. Assay and Biochemical Properties of the Proteinase Inhibitor-inducing Factor, a Wound Hormone. Plant Physiol. 1974 Sep;54(3):328–332. doi: 10.1104/pp.54.3.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ryan C. A. Quantitative determination of soluble cellular proteins by radial diffusion in agar gels containing antibodies. Anal Biochem. 1967 Jun;19(3):434–440. doi: 10.1016/0003-2697(67)90233-3. [DOI] [PubMed] [Google Scholar]
  12. Ryan C. A. The regulation by carbon dioxide of protein synthesis in tomato leaves. Biochem Biophys Res Commun. 1977 Aug 8;77(3):1004–1008. doi: 10.1016/s0006-291x(77)80077-6. [DOI] [PubMed] [Google Scholar]

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