Abstract
Mitochondria isolated from the leaves of several plant species were investigated for the presence of NAD-linked formate dehydrogenase. The NADH produced was oxidized by the electron transport sequence and was coupled to ATP synthesis. The amounts of formate dehydrogenase, and, thereby, the capacity for formate-dependent O2 uptake, varied greatly among species. While no activity was detectable in mitochondria from soybean leaves, the rate of formate oxidation by spinach mitochondria was about one-half the rate of malate oxidation. In spinach, only mitochondria from green tissues oxidized formate. These last two observations raise questions as to the role of this reaction and the possible sources of the formate metabolized.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Douce R., Moore A. L., Neuburger M. Isolation and oxidative properties of intact mitochondria isolated from spinach leaves. Plant Physiol. 1977 Oct;60(4):625–628. doi: 10.1104/pp.60.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardeström P., Bergman A., Ericson I. Oxidation of Glycine via the Respiratory Chain in Mitochondria Prepared from Different Parts of Spinach. Plant Physiol. 1980 Feb;65(2):389–391. doi: 10.1104/pp.65.2.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halliwell B. Oxidation of formate by peroxisomes and mitochondria from spinach leaves. Biochem J. 1974 Jan;138(1):77–85. doi: 10.1042/bj1380077. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leek A. E., Halliwell B., Butt V. S. Oxidation of formate and oxalate in peroxisomal preparations from leaves of spinach beet (Beta vulgaris L.). Biochim Biophys Acta. 1972 Dec 29;286(2):299–311. doi: 10.1016/0304-4165(72)90266-8. [DOI] [PubMed] [Google Scholar]
- Mazelis M. Formate Oxidation by Particulate Preparations from Higher Plants. Plant Physiol. 1960 May;35(3):386–391. doi: 10.1104/pp.35.3.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oliver D. J. Mechanism of decarboxylation of glycine and glycolate by isolated soybean cells. Plant Physiol. 1979 Dec;64(6):1048–1052. doi: 10.1104/pp.64.6.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Somerville C. R., Ogren W. L. Photorespiration-deficient Mutants of Arabidopsis thaliana Lacking Mitochondrial Serine Transhydroxymethylase Activity. Plant Physiol. 1981 Apr;67(4):666–671. doi: 10.1104/pp.67.4.666. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TOLBERT N. E., CLAGETT C. O., BURRIS R. H. Products of the oxidation of glycolic acid and L-lactic acid by enzymes from tobacco leaves. J Biol Chem. 1949 Dec;181(2):905–914. [PubMed] [Google Scholar]
- TOLBERT N. E. Formic acid metabolism in barley leaves. J Biol Chem. 1955 Jul;215(1):27–34. [PubMed] [Google Scholar]
- Zelitch I. The photooxidation of glyoxylate by envelope-free spinach chloroplasts and its relation to photorespiration. Arch Biochem Biophys. 1972 Jun;150(2):698–707. doi: 10.1016/0003-9861(72)90088-4. [DOI] [PubMed] [Google Scholar]