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. 1981 Sep;68(3):731–734. doi: 10.1104/pp.68.3.731

Localization of DNA in Mature and Young Wheat Chloroplasts Using the Fluorescent Probe 4′-6-Diamidino-2-phenylindole 1

Gun Selldén 1,2, Rachel M Leech 1
PMCID: PMC425971  PMID: 16661989

Abstract

The spatial organization of chloroplast DNA in developing and dividing wheat chloroplasts was studied in the light microscope using the fluorescent probe 4′-6-diamidino-2-phenylindole, which binds specifically to DNA.

The DNA of wheat chloroplasts was localized at the periphery of the plastid, frequently in a discrete band. No relocalization of the DNA was observed during plastid replication. This peripheral location of the DNA was shown to differ from the central random location of DNA in tobacco and spinach chloroplasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett J., Radcliffe C. Plastid DNA replication and plastid division in the garden pea. FEBS Lett. 1975 Aug 15;56(2):222–225. doi: 10.1016/0014-5793(75)81096-9. [DOI] [PubMed] [Google Scholar]
  2. Bisalputra T., Bisalputra A. A. The ultrastructure of chloroplast of a brown alga Sphacelaria sp. I. Plastid DNA configuration--the chloroplast genophore. J Ultrastruct Res. 1969 Oct;29(1):151–170. doi: 10.1016/s0022-5320(69)80061-4. [DOI] [PubMed] [Google Scholar]
  3. Bisalputra T., Burton H. The ultrastructure of chloroplast of a brown alga Sphacelaria sp. II. Association between the chloroplast DNA and the photosynthetic lamellae. J Ultrastruct Res. 1969 Nov;29(3):224–235. doi: 10.1016/s0022-5320(69)90103-8. [DOI] [PubMed] [Google Scholar]
  4. Boffey S. A., Ellis J. R., Selldén G., Leech R. M. Chloroplast Division and DNA Synthesis in Light-grown Wheat Leaves. Plant Physiol. 1979 Sep;64(3):502–505. doi: 10.1104/pp.64.3.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boffey S. A., Selldén G., Leech R. M. Influence of Cell Age on Chlorophyll Formation in Light-grown and Etiolated Wheat Seedlings. Plant Physiol. 1980 Apr;65(4):680–684. doi: 10.1104/pp.65.4.680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gibbs S. P., Cheng D., Slankis T. The chloroplast nucleoid in Ochromonas danica. I. Three-dimensional morphology in light- and dark-grown cells. J Cell Sci. 1974 Dec;16(3):557–577. doi: 10.1242/jcs.16.3.557. [DOI] [PubMed] [Google Scholar]
  7. Hajduk S. L. Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum. Science. 1976 Feb 27;191(4229):858–859. doi: 10.1126/science.1251198. [DOI] [PubMed] [Google Scholar]
  8. Herrmann R. G., Kowallik K. V. Multiple amounts of DNA related to the size of chloroplasts. II. Comparison of electron-microscopic and autoradiographic data. Protoplasma. 1970;69(3):365–372. doi: 10.1007/BF01320301. [DOI] [PubMed] [Google Scholar]
  9. Herrmann R. G., Kowallik K. V. Selective presentation of DNA-regions and membranes in chloroplasts and mitochondria. J Cell Biol. 1970 Apr;45(1):198–202. doi: 10.1083/jcb.45.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Herrmann R. G., Possingham J. V. Plastid DNA-the plastome. Results Probl Cell Differ. 1980;10:45–96. doi: 10.1007/978-3-540-38255-3_3. [DOI] [PubMed] [Google Scholar]
  11. James T. W., Jope C. Visualization by fluorescence of chloroplast DNA in higher plants by means of the DNA-specific probe 4'6-diamidino-2-phenylindole. J Cell Biol. 1978 Dec;79(3):623–630. doi: 10.1083/jcb.79.3.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KISLEV N., SWIFT H., BOGORAD L. NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARD. J Cell Biol. 1965 May;25:327–344. doi: 10.1083/jcb.25.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kowallik K. V., Herrmann R. G. Variable amounts of DNA related to the size of chloroplasts. IV. Three-dimensional arrangement of DNA in fully differentiated chloroplasts of Beta vulgaris L. J Cell Sci. 1972 Sep;11(2):357–377. doi: 10.1242/jcs.11.2.357. [DOI] [PubMed] [Google Scholar]
  14. RIS H., PLAUT W. Ultrastructure of DNA-containing areas in the chloroplast of Chlamydomonas. J Cell Biol. 1962 Jun;13:383–391. doi: 10.1083/jcb.13.3.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rose R. J., Possingham J. V. The localization of (3H) thymidine incorporation in the DNA of replicating spinach chloroplasts by electron-microscope autoradiography. J Cell Sci. 1976 Mar;20(2):341–355. doi: 10.1242/jcs.20.2.341. [DOI] [PubMed] [Google Scholar]
  16. Rose R. J. The association of chloroplast DNA with photosynthetic membrane vesicles from spinach chloroplasts. J Cell Sci. 1979 Apr;36:169–183. doi: 10.1242/jcs.36.1.169. [DOI] [PubMed] [Google Scholar]
  17. Russell W. C., Newman C., Williamson D. H. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975 Feb 6;253(5491):461–462. doi: 10.1038/253461a0. [DOI] [PubMed] [Google Scholar]
  18. Williamson D. H., Fennell D. J. The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. Methods Cell Biol. 1975;12:335–351. doi: 10.1016/s0091-679x(08)60963-2. [DOI] [PubMed] [Google Scholar]
  19. Woodcock C. L., Bogorad L. Evidence for variation in the quantity of DNA among plastids of Acetabularia. J Cell Biol. 1970 Feb;44(2):361–375. doi: 10.1083/jcb.44.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woodcock C. L., Fernández-Morán H. Electron microscopy of DNA conformations in spinach chloroplasts. J Mol Biol. 1968 Feb 14;31(3):627–631. doi: 10.1016/0022-2836(68)90435-x. [DOI] [PubMed] [Google Scholar]

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