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. 1981 Oct;68(4):823–826. doi: 10.1104/pp.68.4.823

Cycloheximide Stimulation of Cyanide-Resistant Respiration in Suspension Cultures of Senescent Pear Fruit Cells

Roger J Romani 1,1, Timothy J Bos 1,2, Jean-Claude Pech 1,3
PMCID: PMC425992  PMID: 16662005

Abstract

Pear fruit cells undergoing a period of senescence in auxin-deprived media develop a substantial cyanide resistant respiration in response to the addition of 0.7 to 3.5 micromolar cycloheximide. The inhibitor does not affect overall cellular repiratory activity and titrations with salicylhydroxamic acid reveal that only a minor portion, about 10%, of the alternate pathway is utilized by the cycloheximide-treated senescent cells. The alternate respiratory pathway appears to be of mitochondrial origin but is not induced by chloramphenicol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. I. The steady states of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem. 1973 May 25;248(10):3441–3445. [PubMed] [Google Scholar]
  2. Davies M. E., Exworth C. P. Transient inhibition by cycloheximide of protein synthesis in cultured plant cell suspensions: a dose response paradox. Biochem Biophys Res Commun. 1973 Feb 20;50(4):1075–1080. doi: 10.1016/0006-291x(73)91516-7. [DOI] [PubMed] [Google Scholar]
  3. Dizengremel P., Lance C. Control of Changes in Mitochondrial Activities during Aging of Potato Slices. Plant Physiol. 1976 Aug;58(2):147–151. doi: 10.1104/pp.58.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frenkel C., Klein I., Dilley D. R. Protein synthesis in relation to ripening of pome fruits. Plant Physiol. 1968 Jul;43(7):1146–1153. doi: 10.1104/pp.43.7.1146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Janes H. W., Wiest S. C. Comparison between Aging of Slices and Ethylene Treatment of Whole White Potato Tubers. Plant Physiol. 1980 Jul;66(1):171–174. doi: 10.1104/pp.66.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McMahon D. Cycloheximide is not a specific inhibitor of protein synthesis in vivo. Plant Physiol. 1975 May;55(5):815–821. doi: 10.1104/pp.55.5.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pech J. C., Romani R. J. Senescence of Pear Fruit Cells Cultured in a Continuously Renewed, Auxin-deprived Medium. Plant Physiol. 1979 Nov;64(5):814–817. doi: 10.1104/pp.64.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Romani R. J., Ozelkok S. "Survival" of mitochondria in vitro: physical and energy parameters. Plant Physiol. 1973 Apr;51(4):702–707. doi: 10.1104/pp.51.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Solomos T., Laties G. G. Similarities between the Actions of Ethylene and Cyanide in Initiating the Climacteric and Ripening of Avocados. Plant Physiol. 1974 Oct;54(4):506–511. doi: 10.1104/pp.54.4.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Theologis A., Laties G. G. Respiratory Contribution of the Alternate Path during Various Stages of Ripening in Avocado and Banana Fruits. Plant Physiol. 1978 Aug;62(2):249–255. doi: 10.1104/pp.62.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ziogas B. N., Georgopoulos S. G. Chloramphenicol-induction of a second cyanide-and azide-insensitive mitochondrial pathway in Ustilago maydis. Biochim Biophys Acta. 1980 Sep 5;592(2):223–234. doi: 10.1016/0005-2728(80)90183-8. [DOI] [PubMed] [Google Scholar]

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