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. 1981 Nov;68(5):1175–1179. doi: 10.1104/pp.68.5.1175

Transfer of Oligosaccharide to Protein from a Lipid Intermediate in Plants

Roberto J Staneloni 1,1, Marcelo E Tolmasky 1,2, Claudio Petriella 1, Luis F Leloir 1
PMCID: PMC426064  PMID: 16662070

Abstract

A lipid-bound oligosaccharide was isolated from pea (Pisum sativum) cotyledons incubated with [14C]mannose. The oligosaccharide moiety appeared to be identical with the one obtained from rat liver, known to contain three glucoses, nine mannoses, and two N-acetylglucosamines, and to be involved in protein glycosylation.

Enzymes obtained from soya (Glycine max) roots and developing pea cotyledons were found to catalyze the transfer of oligosaccharide from the lipid intermediate to endogenous protein. The enzymes require Mn2+ and detergent for activity. Evidence is presented indicating that the lipid-bound oligosaccharide with three glucoses is transferred faster than that with less. Some of the peripheral mannoses could be removed without affecting the rate of transfer.

The protein-bound oligosaccharide, formed by incubation of whole cotyledons or by transfer with the enzyme preparation, could be released by protease and endo-β-N-acetylglucosaminidase treatment, as expected for an asparagine-bound high mannose oligosaccharide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beevers L., Mense R. M. Glycoprotein Biosynthesis in Cotyledons of Pisum sativum L: Involvement of Lipid-linked Intermediates. Plant Physiol. 1977 Nov;60(5):703–708. doi: 10.1104/pp.60.5.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Behrens N. H., Parodi A. J., Leloir L. F., Krisman C. R. The role of dolichol monophosphate in sugar transfer. Arch Biochem Biophys. 1971 Apr;143(2):375–383. doi: 10.1016/0003-9861(71)90224-4. [DOI] [PubMed] [Google Scholar]
  3. Brett C. T., Leloir L. F. Dolichyl monophosphate and its sugar derivatives in plants. Biochem J. 1977 Jan 1;161(1):93–101. doi: 10.1042/bj1610093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Browder S. K., Beevers L. Incorporation of [C]Glucosamine and [C]Mannose into Glycolipids and Glycoproteins in Cotyledons of Pisum sativum L. Plant Physiol. 1980 May;65(5):924–930. doi: 10.1104/pp.65.5.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chadwick C. M., Northcote D. H. Partial characterization of the polyisoprenoid carrier of N-acetylglucosamine in Glycine max (soya bean). Biochem J. 1980 Aug 15;190(2):255–261. doi: 10.1042/bj1900255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Das R. C., Heath E. C. Dolichyldiphosphoryloligosaccharide--protein oligosaccharyltransferase; solubilization, purification, and properties. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3811–3815. doi: 10.1073/pnas.77.7.3811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Delmer D. P., Kulow C., Ericson M. C. Glycoprotein Synthesis in Plants: II. Structure of the Mannolipid Intermediate. Plant Physiol. 1978 Jan;61(1):25–29. doi: 10.1104/pp.61.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Forsee W. T., Elbein A. D. Glycoprotein biosynthesis in plants. Demonstration of lipid-linked oligosaccharides of mannose and N-acetylglucosamine. J Biol Chem. 1975 Dec 25;250(24):9283–9293. [PubMed] [Google Scholar]
  9. Forsee W. T., Valkovich G., Elbein A. D. Glycoprotein biosynthesis in plants. Formation of lipid-linked oligosaccharides of mannose and N-acetylglucosamine by mung bean seedlings. Arch Biochem Biophys. 1976 Jun;174(2):469–479. doi: 10.1016/0003-9861(76)90375-1. [DOI] [PubMed] [Google Scholar]
  10. García R. C., Recondo E., Dankert M. Polysaccharide biosynthesis in Acetobacter xylinum. Enzymatic synthesis of lipid diphosphate and monophospate sugars. Eur J Biochem. 1974 Mar 15;43(1):93–105. doi: 10.1111/j.1432-1033.1974.tb03389.x. [DOI] [PubMed] [Google Scholar]
  11. Kobata A. Use of endo- and exoglycosidases for structural studies of glycoconjugates. Anal Biochem. 1979 Nov 15;100(1):1–14. doi: 10.1016/0003-2697(79)90102-7. [DOI] [PubMed] [Google Scholar]
  12. Lehle L., Fartaczek F., Tanner W., Kauss H. Formation of polyprenol-linked mono- and oligosaccharides in Phaseolus aureus. Arch Biochem Biophys. 1976 Aug;175(2):419–426. doi: 10.1016/0003-9861(76)90529-4. [DOI] [PubMed] [Google Scholar]
  13. Lezica R. P., Brett C. T., Martinez P. R., Dankert M. A. A glucose acceptor in plants with the properties of an alpha-saturated polyprenyl-monophosphate. Biochem Biophys Res Commun. 1975 Oct 6;66(3):980–987. doi: 10.1016/0006-291x(75)90736-6. [DOI] [PubMed] [Google Scholar]
  14. Lis H., Sharon N. Soybean agglutinin--a plant glycoprotein. Structure of the carboxydrate unit. J Biol Chem. 1978 May 25;253(10):3468–3476. [PubMed] [Google Scholar]
  15. Parodi A. J., Behrens N. H., Leloir L. F., Carminatti H. The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3268–3272. doi: 10.1073/pnas.69.11.3268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Parodi A. J., Leloir L. F. The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell. Biochim Biophys Acta. 1979 Apr 23;559(1):1–37. doi: 10.1016/0304-4157(79)90006-6. [DOI] [PubMed] [Google Scholar]
  17. Parodi A. J., Staneloni R., Cantarella A. I., Leloir L. F., Behrens N. H., Carminatti H., Levy J. A. Further studies on a glycolipid formed from dolichyl-D-glucosyl monophosphate. Carbohydr Res. 1973 Feb;26(2):393–400. doi: 10.1016/s0008-6215(00)84527-9. [DOI] [PubMed] [Google Scholar]
  18. Sharon N., Lis H. Comparative biochemistry of plant glycoproteins. Biochem Soc Trans. 1979 Aug;7(4):783–799. doi: 10.1042/bst0070783. [DOI] [PubMed] [Google Scholar]
  19. Spiro M. J., Spiro R. G., Bhoyroo V. D. Glycosylation of proteins by oligosaccharide-lipids. Studies on a thyroid enzyme involved in oligosaccharide transfer and the role of glucose in this reaction. J Biol Chem. 1979 Aug 25;254(16):7668–7674. [PubMed] [Google Scholar]
  20. Staneloni R. J., Leloir L. F. Oligosaccharides containing glucose and mannose in glycoproteins of the thyroid gland. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1162–1166. doi: 10.1073/pnas.75.3.1162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Staneloni R. J., Tolmasky M. E., Petriella C., Ugalde R. A., Leloir L. F. Presence in a plant of a compound similar to the dolichyl diphosphate oligosaccharide of animal tissue. Biochem J. 1980 Oct 1;191(1):257–260. doi: 10.1042/bj1910257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Staneloni R. J., Ugalde R. A., Leloir L. F. Addition of glucose to dolichyl diphosphate oligosaccharide and transfer to protein. Eur J Biochem. 1980 Apr;105(2):275–278. doi: 10.1111/j.1432-1033.1980.tb04498.x. [DOI] [PubMed] [Google Scholar]
  23. Turco S. J., Stetson B., Robbins P. W. Comparative rates of transfer of lipid-linked oligosaccharides to endogenous glycoprotein acceptors in vitro. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4411–4414. doi: 10.1073/pnas.74.10.4411. [DOI] [PMC free article] [PubMed] [Google Scholar]

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