Abstract
This study demonstrates germination-induced ultrastructural changes in wheat (Triticum aestivum L. cv Arthur) aleurone cells. Seeds imbided for 4 hours in water contained endoplasmic reticulum (ER) or ER-like membranes as vesicles or as short segments of membrane associated with the spherosomes on the periphery of aleurone grains. Aleurone cells incubated between 8 and 10 hours contained abundant ER membranes mainly associated with the nuclear envelope and, to a lesser extent, with the spherosomes surrounding the aleurone grain. The membranes located on the periphery of the nucleus occurred as regions of stacked cisternae. When aleurone cells were analyzed by morphometry, the increase in ER during incubation was found to be greater than 2-fold. During the same incubation period, other organelles did not change significantly. The early increase in ER was not affected by gibberellin incubation. Thus, the rapid proliferation of ER observed during the early stages of germination in aleurone cells of wheat is not likely to be controlled directly by gibberellin.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ben-Tal Y. An early response to gibberellic Acid not requiring protein synthesis. Plant Physiol. 1974 Dec;54(6):813–816. doi: 10.1104/pp.54.6.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evins W. H., Varner J. E. Hormone-controlled synthesis of endoplasmic reticulum in barley aleurone cells. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1631–1633. doi: 10.1073/pnas.68.7.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Firn R. D., Kende H. Some effects of applied gibberellic Acid on the synthesis and degradation of lipids in isolated barley aleurone layers. Plant Physiol. 1974 Dec;54(6):911–915. doi: 10.1104/pp.54.6.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ho D. T., Varner J. E. Hormonal control of messenger ribonucleic acid metabolism in barley aleurone layers. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4783–4786. doi: 10.1073/pnas.71.12.4783. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson K. D., Kende H. Hormonal Control of Lecithin Synthesis in Barley Aleurone Cells: Regulation of the CDP-Choline Pathway by Gibberellin. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2674–2677. doi: 10.1073/pnas.68.11.2674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones R. L., Chen R. F. Immunohistochemical localization of alpha-amylase in barley aleurone cells. J Cell Sci. 1976 Jan;20(1):183–198. doi: 10.1242/jcs.20.1.183. [DOI] [PubMed] [Google Scholar]
- Koehler D. E., Varner J. E. Hormonal control of orthophosphate incorporation into phospholipids of barley aleurone layers. Plant Physiol. 1973 Sep;52(3):208–214. doi: 10.1104/pp.52.3.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paleg L., Hyde B. Physiological Effects of Gibberellic Acid. VII. Electron Microscopy of Barley Aleurone Cells. Plant Physiol. 1964 Jul;39(4):673–680. doi: 10.1104/pp.39.4.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
- Vigil E. L., Ruddat M. Effect of gibberellic Acid and actinomycin d on the formation and distribution of rough endoplasmic reticulum in barley aleurone cells. Plant Physiol. 1973 Mar;51(3):549–558. doi: 10.1104/pp.51.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]